Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,899,264	G→A	A52V (GCA→GTA)	ygcU ←	putative FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,899,264	0	G	A	98.7%	242.3 / ‑4.4	76	A52V (GCA→GTA)	ygcU	putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (39/36); total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

ACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTGG  >  NC_000913/2899138‑2899389

acaacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCa                                                                                                                   <  1:667046/139‑1 (MQ=255)

acaacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCa                                                                                                                   <  1:147930/139‑1 (MQ=255)

  aacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCaaa                                                                                                                 >  1:74077/1‑139 (MQ=255)

       tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAAt                                                                                                                        >  2:812108/1‑127 (MQ=255)

       tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAAt                                                                                                                        <  1:812108/127‑1 (MQ=255)

       tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCaa                                                                                                                  <  1:730501/133‑1 (MQ=255)

       tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCaa                                                                                                                  >  2:730501/1‑133 (MQ=255)

       tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGtata                                                                                                            >  2:443678/1‑139 (MQ=255)

       tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGtata                                                                                                            >  1:902371/1‑139 (MQ=255)

              cccACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCa                                                                                                     >  1:110556/1‑139 (MQ=255)

               ccACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCAt                                                                                                    >  2:519195/1‑139 (MQ=255)

                 aCCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGa                                                                                                  >  2:262599/1‑139 (MQ=255)

                 aCCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGa                                                                                                  >  1:572391/1‑139 (MQ=255)

                   cTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAat                                                                                                >  1:178979/1‑139 (MQ=255)

                        gTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATTCTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCg                                                                                           >  2:1273993/1‑139 (MQ=255)

                        gTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCg                                                                                           >  1:375711/1‑139 (MQ=255)

                              gAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAAtt                                                                                     <  2:629002/139‑1 (MQ=255)

                                     cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATTCTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa                                                                              <  1:1273993/139‑1 (MQ=255)

                                     cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa                                                                              <  1:519195/139‑1 (MQ=255)

                                     cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa                                                                              <  1:262599/139‑1 (MQ=255)

                                     cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa                                                                              <  2:613273/139‑1 (MQ=255)

                                          cgcgGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGt                                                                         <  1:1028960/139‑1 (MQ=255)

                                               cacaCCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCgg                                                                                                                     <  1:669505/90‑1 (MQ=255)

                                               cacaCCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCgg                                                                                                                     >  2:669505/1‑90 (MQ=255)

                                                acacCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATAc                                                                   <  2:902371/139‑1 (MQ=255)

                                                  acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGtata                                                                                                            <  1:579358/96‑1 (MQ=255)

                                                  acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGtata                                                                                                            >  2:579358/1‑96 (MQ=255)

                                                    cGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGtt                                                               >  1:835703/1‑139 (MQ=255)

                                                     gTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                                              <  2:651352/139‑1 (MQ=255)

                                                     gTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                                              <  2:572391/139‑1 (MQ=255)

                                                        aaTTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCa                                                                                                                   <  2:179702/83‑1 (MQ=255)

                                                        aaTTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCa                                                                                                                   >  1:179702/1‑83 (MQ=255)

                                                              gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCa                                                                                                                   >  1:1304933/1‑77 (MQ=255)

                                                              gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCa                                                                                                                   <  2:1304933/77‑1 (MQ=255)

                                                              gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGtata                                                                                                            >  1:1503154/1‑84 (MQ=255)

                                                              gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGtata                                                                                                            <  2:1503154/84‑1 (MQ=255)

                                                              gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATaa                                                                                                           <  1:1614144/85‑1 (MQ=255)

                                                              gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATaa                                                                                                           >  2:1614144/1‑85 (MQ=255)

                                                                gcgcATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATAc                                                   >  2:671693/1‑139 (MQ=255)

                                                                    aTTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGt                                                                         >  1:1272410/1‑113 (MQ=255)

                                                                    aTTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGt                                                                         <  2:1272410/113‑1 (MQ=255)

                                                                    aTTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGtt                                               <  1:1310523/139‑1 (MQ=255)

                                                                    aTTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGtt                                               >  2:1310523/1‑139 (MQ=255)

                                                                        aTAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCAt                                           <  1:443678/139‑1 (MQ=255)

                                                                         tAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATc                                          >  2:278137/1‑139 (MQ=255)

                                                                              ttCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                                              <  1:1023392/114‑1 (MQ=255)

                                                                              ttCAGCACACGGGATACTTGCTCTGTGGAACCGAGTGTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                                              >  2:1023392/1‑114 (MQ=255)

                                                                                 aGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                                              >  2:778775/1‑111 (MQ=255)

                                                                                 aGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                                              <  1:778775/111‑1 (MQ=255)

                                                                                        gggATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATc                           >  2:1289961/1‑139 (MQ=255)

                                                                                                tGCTCTGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaa                   >  2:76090/1‑139 (MQ=255)

                                                                                                  ctctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCaa                                                                                                                  <  1:13006/42‑1 (MQ=255)

                                                                                                  ctctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCaa                                                                                                                  >  2:13006/1‑42 (MQ=255)

                                                                                                  ctctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTAc                                 <  1:485616/123‑1 (MQ=255)

                                                                                                  ctctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTAc                                 >  2:485616/1‑123 (MQ=255)

                                                                                                   tctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaacaa                <  2:835703/139‑1 (MQ=255)

                                                                                                   tctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaacaa                <  1:1289961/139‑1 (MQ=255)

                                                                                                   tctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaacaa                <  1:190366/139‑1 (MQ=255)

                                                                                                    ctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGt                                                                >  1:1302100/1‑90 (MQ=255)

                                                                                                    ctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGt                                                                <  2:1302100/90‑1 (MQ=255)

                                                                                                    ctGTGGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAAt               >  2:192454/1‑139 (MQ=255)

                                                                                                      gtgGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaacaa                >  1:1409088/1‑136 (MQ=255)

                                                                                                      gtgGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaacaa                <  2:1409088/136‑1 (MQ=255)

                                                                                                       tgGAACCGAGTTTTACGACCGCTACCGGAATCGGCa                                                                                                                   <  1:1264959/36‑1 (MQ=38)

                                                                                                       tgGAACCGAGTTTTACGACCGCTACCGGAATCGGCa                                                                                                                   >  2:1264959/1‑36 (MQ=255)

                                                                                                       tgGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                                              >  2:234925/1‑89 (MQ=255)

                                                                                                       tgGAACCGAGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                                              <  1:234925/89‑1 (MQ=255)

                                                                                                               aGTTTTACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCt    >  1:604601/1‑139 (MQ=255)

                                                                                                                 ttttACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATAc                                                                   <  1:603471/74‑1 (MQ=255)

                                                                                                                 ttttACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATAc                                                                   >  2:603471/1‑74 (MQ=255)

                                                                                                                 ttttACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTgg  >  2:1499574/1‑139 (MQ=255)

                                                                                                                 ttttACGACCGCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTgg  >  2:824786/1‑139 (MQ=255)

                                                                                                                      cGACCGCTACCGGAATCGGCAAAGTATAAATGCCAt                                                                                                    <  2:1117905/36‑1 (MQ=255)

                                                                                                                      cGACCGCTACCGGAATCGGCAAAGTATAAATGCCAt                                                                                                    >  1:1117905/1‑36 (MQ=255)

                                                                                                                           gCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCa                                            >  2:1504072/1‑87 (MQ=255)

                                                                                                                           gCTACCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCa                                            <  1:1504072/87‑1 (MQ=255)

ACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTGG  >  NC_000913/2899138‑2899389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A