Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F1 I1 R1 778 68.7 3228504 93.4% 3015422 109.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,007,887 T→C G126G (GGT→GGC)  ygeX → 2,3‑diaminopropionate ammonia lyase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,007,8870TC100.0% 224.6 / NA 64G126G (GGT→GGC) ygeX2,3‑diaminopropionate ammonia lyase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/28);  total (36/28)

GCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAAC  >  NC_000913/3007759‑3008010
                                                                                                                                |                                                                                                                           
gCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTggg                                                                                                                   >  1:1273118/1‑139 (MQ=255)
     cGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGgcagca                                                                                                              >  1:1329899/1‑139 (MQ=255)
       cTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGcagcaca                                                                                                            >  2:694354/1‑139 (MQ=255)
       cTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGcagcaca                                                                                                            <  2:1306152/139‑1 (MQ=255)
                  tgtgAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGAc                                                                                                 >  1:737778/1‑139 (MQ=255)
                  tgtgAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGAc                                                                                                 >  2:911554/1‑139 (MQ=255)
                    tgAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAg                                                                                               >  2:275261/1‑139 (MQ=255)
                    tgAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAg                                                                                               >  2:902258/1‑139 (MQ=255)
                    tgAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGCCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAg                                                                                               >  2:1111922/1‑139 (MQ=255)
                      aaaaaTATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACa                                                                                                >  2:212582/1‑136 (MQ=255)
                      aaaaaTATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACa                                                                                                <  1:212582/136‑1 (MQ=255)
                      aaaaaTATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGaa                                                                                             >  1:1510784/1‑139 (MQ=255)
                        aaaTATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATg                                                                                           <  1:799954/139‑1 (MQ=255)
                        aaaTATCATCTTGATATCGAAACGCTGTCATTTGAGCAACTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATg                                                                                           <  1:275261/139‑1 (MQ=255)
                              catcTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGa                                                                                     >  1:1396427/1‑139 (MQ=255)
                                   tGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTggg                                                                                                                   >  1:568633/1‑104 (MQ=255)
                                   tGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTggg                                                                                                                   <  2:568633/104‑1 (MQ=255)
                                   tGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTAc                                                                                <  2:1329899/139‑1 (MQ=255)
                                     atatCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACAt                                                                              >  1:314992/1‑139 (MQ=255)
                                          gAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGa                                                                         <  1:694354/139‑1 (MQ=255)
                                               gCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGt                                                                    >  2:1409187/1‑139 (MQ=255)
                                                  gTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGc                                                                                          <  1:1286640/114‑1 (MQ=255)
                                                  gTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGc                                                                                          >  2:1286640/1‑114 (MQ=255)
                                                  gTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                                                                   <  2:1235936/137‑1 (MQ=255)
                                                  gTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                                                                   >  1:1235936/1‑137 (MQ=255)
                                                      tttGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTc                                                             >  2:1211847/1‑139 (MQ=255)
                                                        tGAGCACCTGAAAAATGCCATCGGCGAAAACATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAg                                                           >  2:1364564/1‑139 (MQ=255)
                                                        tGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAg                                                           >  1:495724/1‑139 (MQ=255)
                                                         gAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAgg                                                          >  2:47931/1‑139 (MQ=255)
                                                          aGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                                                                   <  2:1304824/129‑1 (MQ=255)
                                                          aGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                                                                   >  1:1304824/1‑129 (MQ=255)
                                                             aCCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAAcg                                                      >  2:1087277/1‑139 (MQ=255)
                                                               cTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAAcgcg                                                    >  1:902080/1‑139 (MQ=255)
                                                               cTGAAAAATGCCATCGGCG‑AAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGt                                                   >  1:521885/1‑139 (MQ=255)
                                                                tGAAAAATGCCATCTGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCTGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCTGAAAGGTTCTGCTCAGCAACGCGt                                                   <  2:984920/139‑1 (MQ=255)
                                                                    aaaTGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATG‑CGAAAGGTTCTGCTCAGGAACGCGTTGACg                                              <  1:902258/139‑1 (MQ=255)
                                                                      aTGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGc                                             <  2:737778/139‑1 (MQ=255)
                                                                       tGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGcc                                            >  2:545148/1‑139 (MQ=255)
                                                                        gCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCa                                           <  1:1364564/139‑1 (MQ=255)
                                                                            tCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                                                                   >  1:295763/1‑111 (MQ=255)
                                                                            tCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                                                                   <  2:295763/111‑1 (MQ=255)
                                                                             cGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTg                                      <  1:1409187/139‑1 (MQ=255)
                                                                                  aaaaaaTGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCt                                 >  1:274745/1‑139 (MQ=255)
                                                                                    aaaaTGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCg                               <  1:911554/139‑1 (MQ=255)
                                                                                       aTGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTg                            <  2:314992/139‑1 (MQ=255)
                                                                                         gACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACa                                                                                                >  1:1184215/1‑69 (MQ=255)
                                                                                         gACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACa                                                                                                <  2:1184215/69‑1 (MQ=255)
                                                                                          aCTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCg                                                                                         >  2:1030554/1‑75 (MQ=255)
                                                                                          aCTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCg                                                                                         <  1:1030554/75‑1 (MQ=255)
                                                                                              tCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTg                     >  1:1556945/1‑139 (MQ=255)
                                                                                              tCGCGACCACCACCGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTg                     >  2:962070/1‑139 (MQ=255)
                                                                                                      accaccGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCa             <  2:1510784/139‑1 (MQ=255)
                                                                                                      accaccGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCa             <  2:1596467/139‑1 (MQ=255)
                                                                                                        caccGACGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACg           >  1:1517018/1‑139 (MQ=255)
                                                                                                             aCGGCAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGatat      >  1:85034/1‑139 (MQ=255)
                                                                                                              cGGCAACCACGGACGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTAGGTGCCGAGTGCATCGTCACGGATATg     <  1:962070/139‑1 (MQ=255)
                                                                                                                 cAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAAc  <  1:67373/139‑1 (MQ=255)
                                                                                                                 cAACCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAAc  <  2:988677/139‑1 (MQ=255)
                                                                                                                  aaCCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTg                                      >  2:562015/1‑102 (MQ=255)
                                                                                                                  aaCCACGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTg                                      <  1:562015/102‑1 (MQ=255)
                                                                                                                       cGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATg                                                                                           >  2:134545/1‑44 (MQ=255)
                                                                                                                       cGGGCGCGGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATg                                                                                           <  1:134545/44‑1 (MQ=255)
                                                                                                                           cgcgGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACa                                                                               <  2:1190191/52‑1 (MQ=255)
                                                                                                                           cgcgGCGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACa                                                                               >  1:1190191/1‑52 (MQ=255)
                                                                                                                                |                                                                                                                           
GCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAAC  >  NC_000913/3007759‑3008010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A