Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I1 R1
|
778 |
68.7 |
3228504 |
93.4% |
3015422 |
109.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,082,796 |
G→A |
intergenic (+125/+81) |
loiP → / ← speB |
Phe‑Phe periplasmic metalloprotease, OM lipoprotein, low salt‑inducible, Era‑binding heat shock protein/agmatinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,082,796 | 0 | G | A | 100.0%
| 189.8
/ NA
| 59 | intergenic (+125/+81) | loiP/speB | Phe‑Phe periplasmic metalloprotease, OM lipoprotein, low salt‑inducible, Era‑binding heat shock protein/agmatinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (35/24); total (35/24) |
GGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCC > NC_000913/3082660‑3082931
|
gggATTAAGAAAAGCCTTGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCAAGCCGCCTCTGACATGAATGGCACATTTTTTTCCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTTACCGGCCTACGGGTAcc < 1:989279/139‑1 (MQ=38)
aGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTAT‑CGGCCTACGGGTACCGAACGTAg < 2:948982/139‑1 (MQ=255)
cccggtggtGTCGAACGTAGGTCAGATACGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATCCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGa > 1:882728/4‑139 (MQ=255)
gtggtgTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAg > 1:1006058/1‑139 (MQ=255)
aaCGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCAcg > 1:461592/1‑139 (MQ=255)
aCGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCAcgc < 2:1006058/139‑1 (MQ=255)
ggTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCAt > 2:1578275/1‑139 (MQ=255)
gTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGt < 2:681890/115‑1 (MQ=255)
gTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGt > 1:681890/1‑115 (MQ=255)
gCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCAcgccg > 1:1060758/1‑125 (MQ=255)
gCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCAcgccg < 2:1060758/125‑1 (MQ=255)
gTTTACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATgg < 2:1037497/139‑1 (MQ=255)
aTGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCAcg < 2:919975/102‑1 (MQ=255)
aTGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCAcg > 1:919975/1‑102 (MQ=255)
cacaTTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCgcatcgcatc < 1:1163575/139‑1 (MQ=255)
cacaTTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCgcatcgcatc < 2:461592/139‑1 (MQ=255)
ttGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTg > 2:313744/1‑139 (MQ=255)
ccTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACt < 2:882728/139‑1 (MQ=255)
ttGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGcgca < 2:1540862/119‑1 (MQ=255)
ttGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGcgca > 1:1540862/1‑119 (MQ=255)
ttGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCg > 2:217798/1‑139 (MQ=255)
gtgCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATgg > 1:1306534/1‑98 (MQ=255)
gtgCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATgg < 2:1306534/98‑1 (MQ=255)
gtgCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGcgcatcgcat > 1:1132215/1‑123 (MQ=255)
gtgCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGcgcatcgcat < 2:451191/123‑1 (MQ=255)
gtgCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGcgcatcgcat > 1:451191/1‑123 (MQ=255)
gtgCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGcgcatcgcat < 2:1132215/123‑1 (MQ=255)
gtgCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGcc > 1:704522/1‑139 (MQ=255)
gtgCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGcc > 2:1335535/1‑139 (MQ=255)
tgcacATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGAc < 2:487121/85‑1 (MQ=255)
tgCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGccc > 1:1470085/1‑139 (MQ=255)
tgCACATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGAc > 1:487121/1‑89 (MQ=255)
aTTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCttttt > 2:1477722/1‑139 (MQ=255)
tGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACAGGTACTGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTcg > 2:1076790/1‑139 (MQ=255)
cGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTcgccg > 2:322552/1‑139 (MQ=255)
acagatgcTGGCCCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGGCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCCTTTTCGCCGCCTg < 2:383333/136‑1 (MQ=39)
gcgatgcTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGa < 1:1477722/139‑1 (MQ=255)
gatgcTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCTTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGa < 1:1294086/137‑1 (MQ=255)
gatgcTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGa > 2:1294086/1‑137 (MQ=255)
tgcTGGCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAatat > 2:553788/1‑139 (MQ=255)
ggCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATgg < 2:752968/74‑1 (MQ=255)
ggCGCATCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATgg > 1:752968/1‑74 (MQ=255)
aTCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCAttt > 2:978243/1‑139 (MQ=255)
tCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATgg < 2:1415580/68‑1 (MQ=255)
tCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATgg > 1:1415580/1‑68 (MQ=255)
tCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTc > 1:91393/1‑139 (MQ=255)
tCTTATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCCCCCCTTTTCCCCCCCTAATTATCCacaattgt > 1:383333/1‑132 (MQ=37)
ttATCCGGCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTcca > 2:273979/1‑139 (MQ=255)
gCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTg < 2:1557727/74‑1 (MQ=255)
gCCTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTg > 1:1557727/1‑74 (MQ=255)
ccTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTccagcgccagc > 2:477922/1‑139 (MQ=255)
ccTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTccagcgccagc > 1:886796/1‑139 (MQ=255)
cTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTccagcgccagcg > 1:333765/1‑139 (MQ=255)
cTACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTccagcgccagcg < 1:273979/139‑1 (MQ=255)
tACGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACACGTGCGCATCGCCTCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGt > 1:257954/1‑139 (MQ=255)
cGGGTACCGAACGTAGGTCGGATAAGGCGTTCAcgc < 2:164295/36‑1 (MQ=17)
cGGGTACCGAACGTAGGTCGGATAAGGCGTTCAcgc > 1:164295/1‑36 (MQ=17)
cGGGTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTg > 1:463568/1‑139 (MQ=255)
ggTACCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGcc < 1:1335535/139‑1 (MQ=255)
|
GGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCATCGCATCTGGTGCTTACTCGCCCTTTTTCGCCGCCTGAATATACAGCATTTCCAGCGCCAGCGTTGCC > NC_000913/3082660‑3082931
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
