Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,482,967	T→C	L560P (CTG→CCG)	yheS →	ABC‑F family protein predicted regulatory ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,482,967	0	T	C	100.0%	160.4 / NA	45	L560P (CTG→CCG)	yheS	ABC‑F family protein predicted regulatory ATPase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/25); total (20/25)

TATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCA  >  NC_000913/3482831‑3483099

tATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGc                                                                                                                                    >  1:1593754/1‑139 (MQ=255)

  tCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGt                                                                                                                                  >  1:332308/1‑139 (MQ=255)

         tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAgaga                                                                                                                           >  1:516534/1‑139 (MQ=255)

         tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGT‑AAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGAt                                                                                                                          >  2:1394968/1‑139 (MQ=255)

           gTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGAtt                                                                                                                         >  2:604491/1‑139 (MQ=255)

                gCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCg                                                                                                                    >  1:440171/1‑139 (MQ=255)

                      tACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGa                                                                                                              >  1:570109/1‑139 (MQ=255)

                      tACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGa                                                                                                              >  2:1146215/1‑139 (MQ=255)

                        cAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGaaa                                                                                                            <  2:516534/139‑1 (MQ=255)

                              cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAgaga                                                                                                      >  2:78217/1‑139 (MQ=255)

                                ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAaga                                                                                                        >  1:248776/1‑135 (MQ=255)

                                ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAaga                                                                                                        <  2:248776/135‑1 (MQ=255)

                                        aGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGT‑AAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTg                                                                                           <  1:1394968/139‑1 (MQ=255)

                                             gacgaAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcg                                                                                       <  2:369977/139‑1 (MQ=255)

                                              acgaAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcgc                                                                                      <  1:492922/139‑1 (MQ=255)

                                                       cAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGgc                                                                             <  1:1146215/139‑1 (MQ=255)

                                                             agaATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGc                                                                       <  2:440171/139‑1 (MQ=255)

                                                                      aCAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGaa                                                              >  1:1502104/1‑139 (MQ=255)

                                                                         gcgcCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCg                                                                                                                    >  2:803634/1‑82 (MQ=255)

                                                                         gcgcCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCg                                                                                                                    <  1:803634/82‑1 (MQ=255)

                                                                                 gCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACa                                                   <  1:170298/139‑1 (MQ=255)

                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                <  2:1593754/139‑1 (MQ=255)

                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                <  1:1559668/139‑1 (MQ=255)

                                                                                     gTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                               <  2:570109/139‑1 (MQ=255)

                                                                                     gTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                               <  1:604491/139‑1 (MQ=255)

                                                                                     gTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                               <  1:526415/139‑1 (MQ=255)

                                                                                     gTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                               <  2:1196569/139‑1 (MQ=255)

                                                                                     gTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                               <  2:332308/139‑1 (MQ=255)

                                                                                       aaaGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAAc                                             <  2:842086/139‑1 (MQ=255)

                                                                                               gAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAc                                     >  2:895958/1‑139 (MQ=255)

                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCa                                   <  1:748724/135‑1 (MQ=255)

                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCa                                   >  2:748724/1‑135 (MQ=255)

                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGc                               >  1:1409499/1‑139 (MQ=255)

                                                                                                          aaGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGAGTGCCCGTCTGGAAAAAGAGGTGGAGGAGCTGAACGAGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCAAACTGTATGACCAGAGCCGTaa                          >  1:70295/1‑139 (MQ=255)

                                                                                                            gctgagctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAg                        <  1:444306/139‑1 (MQ=255)

                                                                                                             ctgagctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCagag                                >  1:838903/1‑130 (MQ=255)

                                                                                                             ctgagctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCagag                                <  2:838903/130‑1 (MQ=255)

                                                                                                                agctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAc                                     >  2:304588/1‑122 (MQ=255)

                                                                                                                agctgCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAc                                     <  1:304588/122‑1 (MQ=255)

                                                                                                                     cgtgcgCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                >  2:1566267/1‑106 (MQ=255)

                                                                                                                     cgtgcgCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                <  1:1566267/106‑1 (MQ=255)

                                                                                                                         cgcAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgc           <  1:859398/139‑1 (MQ=255)

                                                                                                                          gcAAA‑CCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgcct         <  2:77569/139‑1 (MQ=255)

                                                                                                                                 cAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCctgcctgc   <  2:1409499/139‑1 (MQ=255)

                                                                                                                                  aGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCa  >  1:97843/1‑139 (MQ=255)

TATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCA  >  NC_000913/3482831‑3483099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A