Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,018,760	G→A	476 (TAG→TAA)	rmuC →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,018,760	0	G	A	100.0%	256.9 / NA	79	476 (TAG→TAA)	rmuC	DNA recombination protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (43/36); total (43/36)

AAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAGCCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTGG  >  NC_000913/4018626‑4018889

aaaCGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCa                                                                                                                               >  1:463414/1‑139 (MQ=255)

 aaCGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCAt                                                                                                                              <  1:534990/139‑1 (MQ=255)

  aCGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCAtt                                                                                                                             >  2:35518/1‑139 (MQ=255)

               ccGGATTTGGCTGAACAGGCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAg                                                                                                                <  2:873004/139‑1 (MQ=255)

                  gATTTGGCTGAACAGGCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCg                                                                                                             >  2:1231671/1‑139 (MQ=255)

                      tGGCTGAACAGGCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTa                                                                                                         <  2:415367/139‑1 (MQ=255)

                        gCTGAACAGGCGGTGCGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGa                                                                                                       >  1:1249335/1‑139 (MQ=255)

                                 gCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAggg                                                                                              >  2:1555973/1‑139 (MQ=255)

                                 gCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAggg                                                                                              >  2:314381/1‑139 (MQ=255)

                                  cGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCAtt                                                                                                                             <  1:1610016/107‑1 (MQ=255)

                                  cGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCAtt                                                                                                                             >  2:1610016/1‑107 (MQ=255)

                                       aGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGa                                                                                        >  1:310912/1‑139 (MQ=255)

                                       aGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGa                                                                                        <  1:1519929/139‑1 (MQ=255)

                                             gATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                  <  1:1231671/139‑1 (MQ=255)

                                                  agagTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTg                                                                             >  2:261250/1‑139 (MQ=255)

                                                       aTCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                  <  2:802389/129‑1 (MQ=255)

                                                       aTCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                  >  1:802389/1‑129 (MQ=255)

                                                         cGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACt                                                                      >  1:507329/1‑139 (MQ=255)

                                                              tcggtcggTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGag                                                                                                                        >  2:777216/1‑84 (MQ=255)

                                                              tcggtcggTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGag                                                                                                                        <  1:777216/84‑1 (MQ=255)

                                                              tcggtcggTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTgg                                                                 >  1:30194/1‑139 (MQ=255)

                                                                ggtcggtTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                  >  1:1005116/1‑120 (MQ=255)

                                                                ggtcggtTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                  <  1:93211/120‑1 (MQ=255)

                                                                ggtcggtTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                  <  2:1005116/120‑1 (MQ=255)

                                                                ggtcggtTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                  >  2:93211/1‑120 (MQ=255)

                                                                            agcagcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTa                                                                          >  2:401278/1‑116 (MQ=255)

                                                                            agcagcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTa                                                                          <  1:401278/116‑1 (MQ=255)

                                                                               agcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAg                                                <  1:261250/139‑1 (MQ=255)

                                                                               agcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAg                                                <  1:859599/139‑1 (MQ=255)

                                                                               agcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAg                                                <  1:35518/139‑1 (MQ=255)

                                                                               agcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAg                                                <  1:634124/139‑1 (MQ=255)

                                                                                gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTggg                                                                                                                          <  1:94242/64‑1 (MQ=255)

                                                                                gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTggg                                                                                                                          >  2:94242/1‑64 (MQ=255)

                                                                                gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAg                                                                                                        <  2:941632/82‑1 (MQ=255)

                                                                                gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAg                                                                                                        >  1:941632/1‑82 (MQ=255)

                                                                                gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGcc                                                                                    >  1:1132146/1‑102 (MQ=255)

                                                                                gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGcc                                                                                    <  2:1132146/102‑1 (MQ=255)

                                                                                gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                  <  2:484670/104‑1 (MQ=255)

                                                                                gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                  >  1:484670/1‑104 (MQ=255)

                                                                                gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGc                                               >  2:323325/1‑139 (MQ=255)

                                                                                gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGc                                               >  1:851358/1‑139 (MQ=255)

                                                                                gcCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGc                                               <  2:1249335/139‑1 (MQ=255)

                                                                                   gAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAgg                                            <  2:463414/139‑1 (MQ=255)

                                                                                         tgaAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCCTTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTga                                      <  1:314381/139‑1 (MQ=255)

                                                                                           aaGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCAtt                                                                                                                             <  1:1050916/50‑1 (MQ=255)

                                                                                           aaGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCAtt                                                                                                                             >  2:1050916/1‑50 (MQ=255)

                                                                                                tATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGcc                                                                                    >  1:310734/1‑86 (MQ=255)

                                                                                                tATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGcc                                                                                    <  2:310734/86‑1 (MQ=255)

                                                                                                 aTCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGAc                                                                                       >  2:957425/1‑82 (MQ=255)

                                                                                                 aTCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGAc                                                                                       <  1:957425/82‑1 (MQ=255)

                                                                                                  tCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                  <  2:73543/86‑1 (MQ=255)

                                                                                                  tCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCa                                                                                  >  1:73543/1‑86 (MQ=255)

                                                                                                  tCAACGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGa                             <  1:323325/139‑1 (MQ=255)

                                                                                                    aaCGCGATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATa                           <  1:1250593/139‑1 (MQ=255)

                                                                                                       gcgATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAg                                                                                                                >  2:856485/1‑51 (MQ=255)

                                                                                                       gcgATGATGAATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAg                                                                                                                <  1:856485/51‑1 (MQ=255)

                                                                                                           tgatgaATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTg                                       <  2:1493416/120‑1 (MQ=255)

                                                                                                           tgatgaATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTg                                       >  1:1493416/1‑120 (MQ=255)

                                                                                                           tgatgaATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTcaca                    >  2:1554062/1‑139 (MQ=255)

                                                                                                           tgatgaATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTcaca                    >  2:475273/1‑139 (MQ=255)

                                                                                                           tgatgaATATAATCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACATCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTcaca                    >  1:475780/1‑139 (MQ=255)

                                                                                                                     aaTCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTa                                                                          >  1:749801/1‑75 (MQ=255)

                                                                                                                     aaTCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTa                                                                          <  2:749801/75‑1 (MQ=255)

                                                                                                                       tCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAg                                                >  1:1392081/1‑99 (MQ=255)

                                                                                                                       tCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAg                                                <  2:1392081/99‑1 (MQ=255)

                                                                                                                       tCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGAt                                   >  2:1592877/1‑112 (MQ=255)

                                                                                                                       tCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGAt                                   <  1:1592877/112‑1 (MQ=255)

                                                                                                                       tCAGCAGTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCa        >  2:799146/1‑139 (MQ=255)

                                                                                                                       tCAGCAGTCGCGCTAAACCATTGGGAGTAGTTAAGCCGGGTAGAAGTCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCa        >  1:901742/1‑139 (MQ=255)

                                                                                                                          gcagTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACtt     >  1:719455/1‑139 (MQ=255)

                                                                                                                           cagTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGAt                            <  1:341481/115‑1 (MQ=255)

                                                                                                                           cagTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGAt                            >  2:341481/1‑115 (MQ=255)

                                                                                                                           cagTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACttt    >  2:126796/1‑139 (MQ=255)

                                                                                                                            agTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTg   >  2:222168/1‑139 (MQ=255)

                                                                                                                             gTCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTgg  >  1:995733/1‑139 (MQ=255)

                                                                                                                              tCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTtgatg                                                  >  1:1563047/1‑90 (MQ=255)

                                                                                                                              tCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTtgatg                                                  <  2:1563047/90‑1 (MQ=255)

                                                                                                                              tCGCGCTAACCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCa                                              >  2:1151638/1‑94 (MQ=255)

                                                                                                                              tCGCGCTAACCCATTGGCAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCa                                              <  1:1151638/94‑1 (MQ=255)

AAACGCGAGATTAATCCGGATTTGGCTGAACAGGCGGTGAGCCAGGATGAAGAGTATCGACTTCGGTCGGTTCCGGAGCAGCCGAATGATGAAGCTTATCAACGCGATGATGAATATAATCAGCAGTCGCGCTAGCCCATTGGGAGTAGTTAAGCCGGGTAGAAATCTAGGGCATCGACGCCCAATCTGTTACACTTCTGGAACAATTTTTTGATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTGG  >  NC_000913/4018626‑4018889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A