Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I1 R1 765 39.6 1867486 94.4% 1762906 108.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,911,417 C→T *745* (TAG→TAA)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,911,4170CT98.8% 275.1 / NA 85*745* (TAG→TAA) relA(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (52/32);  minor base A (1/0);  total (53/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGT  >  NC_000913/2911315‑2911545
                                                                                                      |                                                                                                                                
aaCGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATcc                                                                                              >  1:688788/1‑139 (MQ=255)
                   gATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGAcgcgcg                                                                                                          >  1:19085/1‑108 (MQ=255)
                   gATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGAcgcgcg                                                                                                          <  2:19085/108‑1 (MQ=255)
                        tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGc                                                                      >  2:882531/1‑139 (MQ=255)
                        tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGc                                                                      >  1:538029/1‑139 (MQ=255)
                         gagacagtCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTAGATTGTAGATACGAGCAAATTTCGTCTTAACTCCCGTGCATCCGACGCGCGGCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                     <  1:592088/132‑1 (MQ=255)
                                tCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGccc                                                              >  2:853595/1‑139 (MQ=255)
                                tCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGccc                                                              >  1:29473/1‑139 (MQ=255)
                                  cTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCa                                                            <  1:132251/139‑1 (MQ=255)
                                   tCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAg                                                           <  2:538029/139‑1 (MQ=255)
                                           cTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGTTAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcc                                                               <  2:212624/127‑1 (MQ=255)
                                           cTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcc                                                               >  1:212624/1‑127 (MQ=255)
                                           cTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACATGGTTGAGTTCACCCAGCACGCGCCCCAGcctgtctc                                                   >  2:592088/1‑132 (MQ=255)
                                            tATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAg                                                  >  2:726726/1‑139 (MQ=255)
                                                  atGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTa                                            <  1:726726/139‑1 (MQ=255)
                                                  atGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTa                                            <  2:29473/139‑1 (MQ=255)
                                                   tGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTgg                                                                                     <  1:714639/97‑1 (MQ=255)
                                                   tGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTgg                                                                                     >  2:714639/1‑97 (MQ=255)
                                                     tAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGa                                                                                                                >  1:149319/1‑68 (MQ=255)
                                                     tAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGa                                                                                                                <  2:149319/68‑1 (MQ=255)
                                                     tAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCa                                                   >  2:184434/1‑129 (MQ=255)
                                                     tAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCa                                                   <  1:184434/129‑1 (MQ=255)
                                                      aGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTTGGCTTAACTCCCGTGCAACCGACGCGCGt                                                                                                         >  2:658349/1‑74 (MQ=255)
                                                      aGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGt                                                                                                         <  1:658349/74‑1 (MQ=255)
                                                        aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGAcg                                                                                                              >  2:66304/1‑67 (MQ=255)
                                                        aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGAcg                                                                                                              <  1:66304/67‑1 (MQ=255)
                                                        aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAAtctc                                      >  1:464300/1‑139 (MQ=255)
                                                        aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAAtctc                                      >  1:599328/1‑139 (MQ=255)
                                                            aGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATa                                                                                                    >  1:442385/1‑73 (MQ=255)
                                                            aGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATa                                                                                                    <  2:442385/73‑1 (MQ=255)
                                                            aGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCa                                                   >  1:362909/1‑122 (MQ=255)
                                                            aGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCa                                                   <  2:362909/122‑1 (MQ=255)
                                                            aGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATg                                  >  2:600632/1‑139 (MQ=255)
                                                             gTATATATCAATCTACATTGTATATACGAGCAAATTTCGGCATAACTCCCGCGCAACCGACGCGCGTCGATAACATCCGGAACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAAGCTCAATgg                                 >  2:574115/1‑139 (MQ=255)
                                                               atatatCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTc                               >  1:456227/1‑139 (MQ=255)
                                                                tatatCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCa                              >  2:917807/1‑139 (MQ=255)
                                                                  tatCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGAcgcgcg                                                                                                          >  2:762792/1‑61 (MQ=255)
                                                                  tatCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGAcgcgcg                                                                                                          <  1:762792/61‑1 (MQ=255)
                                                                  tatCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGt                                                                                    >  2:385415/1‑83 (MQ=255)
                                                                  tatCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGt                                                                                    <  1:385415/83‑1 (MQ=255)
                                                                   atCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCAcg                                                                   >  2:387774/1‑99 (MQ=255)
                                                                   atCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCAcg                                                                   <  1:387774/99‑1 (MQ=255)
                                                                   atCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTa                                            <  2:567604/122‑1 (MQ=255)
                                                                   atCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTa                                            >  1:567604/1‑122 (MQ=255)
                                                                   atCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGt                           >  1:335643/1‑139 (MQ=255)
                                                                        tCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGAcg                                                                                                              <  1:169109/51‑1 (MQ=255)
                                                                        tCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGAcg                                                                                                              >  2:169109/1‑51 (MQ=255)
                                                                        tCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGt                                                                                    <  1:329029/77‑1 (MQ=255)
                                                                        tCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGt                                                                                    >  2:329029/1‑77 (MQ=255)
                                                                        tCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATg                      >  1:713908/1‑139 (MQ=255)
                                                                        tCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATg                      >  2:22264/1‑139 (MQ=255)
                                                                        tCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATg                      >  1:516051/1‑139 (MQ=255)
                                                                        tCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATg                      >  1:405307/1‑139 (MQ=255)
                                                                        tCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATg                      >  1:529922/1‑139 (MQ=255)
                                                                          tACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGAcg                                                                                                              <  1:374658/49‑1 (MQ=255)
                                                                          tACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGAcg                                                                                                              >  2:374658/1‑49 (MQ=255)
                                                                          tACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGt                    >  2:144112/1‑139 (MQ=255)
                                                                            cATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGAcgcg                                                                                                            >  2:308077/1‑49 (MQ=255)
                                                                            cATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGAcgcg                                                                                                            <  1:308077/49‑1 (MQ=255)
                                                                            cATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGa                        <  2:33652/133‑1 (MQ=255)
                                                                            cATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGa                        >  1:33652/1‑133 (MQ=255)
                                                                            cATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCg                  >  1:316918/1‑139 (MQ=255)
                                                                              ttGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGcc                <  2:464300/139‑1 (MQ=255)
                                                                                 tAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATc                                                                                               >  1:81434/1‑57 (MQ=255)
                                                                                 tAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATc                                                                                               <  2:81434/57‑1 (MQ=255)
                                                                                 tAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGc                 <  1:507544/135‑1 (MQ=255)
                                                                                 tAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGc                 >  2:507544/1‑135 (MQ=255)
                                                                                 tAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGt             >  2:255575/1‑139 (MQ=255)
                                                                                      aCGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGc                                                                                           >  2:857782/1‑56 (MQ=255)
                                                                                      aCGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGc                                                                                           <  1:857782/56‑1 (MQ=255)
                                                                                      aCGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGt                                                                                    <  2:167838/63‑1 (MQ=255)
                                                                                      aCGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGt                                                                                    >  1:167838/1‑63 (MQ=255)
                                                                                      aCGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTg        >  2:16633/1‑139 (MQ=255)
                                                                                      aCGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTg        >  1:629769/1‑139 (MQ=255)
                                                                                         aGCAAATTTCGGCTTAACTCCCGTGCAACCGAcgc                                                                                                             <  2:384539/35‑1 (MQ=255)
                                                                                         aGCAAATTTCGGCTTAACTCCCGTGCAACCGAcgc                                                                                                             >  1:384539/1‑35 (MQ=255)
                                                                                         aGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcccc                                                             >  1:807803/1‑83 (MQ=255)
                                                                                         aGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcccc                                                             <  2:807803/83‑1 (MQ=255)
                                                                                         aGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGttt     >  2:449532/1‑139 (MQ=255)
                                                                                         aGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGttt     >  2:180097/1‑139 (MQ=255)
                                                                                           cAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTgg   >  2:506099/1‑139 (MQ=255)
                                                                                           cAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTgg   >  1:165784/1‑139 (MQ=255)
                                                                                           cAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTgg   >  2:915632/1‑139 (MQ=255)
                                                                                            aaaTTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGgt  <  1:144112/139‑1 (MQ=255)
                                                                                                   ggCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAg                                                           <  1:605292/75‑1 (MQ=255)
                                                                                                      |                                                                                                                                
AACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGT  >  NC_000913/2911315‑2911545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A