Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,012,261 |
T→C |
G267G (GGT→GGC) |
yqeA → |
putative amino acid kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,012,261 | 0 | T | C | 100.0%
| 147.4
/ NA
| 42 | G267G (GGT→GGC) | yqeA | putative amino acid kinase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/22); total (20/22) |
TGCCGATATGCTGATCATTCTGACTGCGGTAGAAAAAGTGGCCATTAACTTCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGT > NC_000913/3012126‑3012387
|
tGCCGATATGCTGATCATTCTGACTGCGGTAGAAAAAGTGGCCATTAACTTCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGctct > 2:16215/1‑139 (MQ=255)
cGATATGCTTGTCATTCTGATTGCGGTAGTAAAAGTGGCCATTAACTTCGGTAAAGAGATTGACCAGTGGCTCGCTCGCCTGTCATTAAGTGATGCTGAACGCTTTATCTAAGAAGGTCATTTTGCGAAAGGCTCTagg < 2:451565/139‑3 (MQ=255)
atatGCTGATCATTCTGACTGCGGTAGAAAAAGTGGCCATTAACTTCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTAtgct > 1:705191/1‑139 (MQ=255)
aTCATTCTGACTGCGGTAGAAAAAGTGGCCATTAACTTCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAg > 1:636749/1‑139 (MQ=255)
aCTGCGGTAGAAAAAGTGGCCATTAACTTCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTg > 1:84431/1‑139 (MQ=255)
cTGCGGTAGAAAAAGTGGCCATTAACTTCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTgc > 2:295185/1‑139 (MQ=255)
ggTAGAAAAAGTGGCCATTAACTTCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTgccgcc > 1:764723/1‑139 (MQ=255)
gCCATTAACTTCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTgctcgct < 1:16215/139‑1 (MQ=255)
gCCATTAACTTCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTgctcgct < 2:764723/139‑1 (MQ=255)
tCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAgg < 2:705191/139‑1 (MQ=255)
gaATGACCAGTGGCTCGATCGCCTGTCATTAAGTGCTGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGACGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCa < 2:244974/139‑1 (MQ=255)
aTGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACt < 2:84431/139‑1 (MQ=255)
aTGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACt < 2:636749/139‑1 (MQ=255)
gAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGa < 2:931244/139‑1 (MQ=255)
tGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCg > 1:62611/1‑139 (MQ=255)
ggCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAgg > 1:40751/1‑118 (MQ=255)
ggCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAgg < 2:40751/118‑1 (MQ=255)
cGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCa < 2:62611/139‑1 (MQ=255)
tAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTAGGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAaaga < 2:44748/139‑1 (MQ=255)
gTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGcc > 1:408862/1‑55 (MQ=255)
gTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGcc < 2:408862/55‑1 (MQ=255)
aTGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTgctcg > 1:69789/1‑82 (MQ=255)
aTGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTgctcg < 2:69789/82‑1 (MQ=255)
aTGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAg > 2:720095/1‑102 (MQ=255)
aTGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAg < 1:720095/102‑1 (MQ=255)
tGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGACTAAAGTGGAAGCTGCCTCCTCGTTTGCGCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGAtt > 1:32892/1‑139 (MQ=255)
gCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTAcc > 2:493922/1‑111 (MQ=255)
gCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTg > 1:283586/1‑139 (MQ=255)
gCTGAACGCTTTATCGAAGAAGGTCATCTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTAcc < 1:493922/111‑1 (MQ=255)
aaCGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAgg > 1:512921/1‑88 (MQ=255)
aaCGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAgg < 2:512921/88‑1 (MQ=255)
aaCGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGAtt < 1:545517/134‑1 (MQ=255)
aaCGCTTTATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGAtt > 2:545517/1‑134 (MQ=255)
tttATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTcgcg < 2:817887/79‑1 (MQ=255)
tttATCGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTcgcg > 1:817887/1‑79 (MQ=255)
cGAAGAAGGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTAGCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAg > 2:603728/1‑137 (MQ=255)
cGAAGAAGGCCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGTCAAAACAg < 1:603728/137‑1 (MQ=255)
aagaagGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAAc > 1:407946/1‑133 (MQ=255)
aagaagGTCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAAc < 2:407946/133‑1 (MQ=255)
tCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAgg > 1:66266/1‑69 (MQ=255)
tCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAgg < 2:66266/69‑1 (MQ=255)
tCATTTTGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGAtt > 1:171771/1‑139 (MQ=255)
ttttGCGAAAGGCTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGt < 2:92130/139‑1 (MQ=255)
|
TGCCGATATGCTGATCATTCTGACTGCGGTAGAAAAAGTGGCCATTAACTTCGGTAAAGAGAATGAACAGTGGCTCGATCGCCTGTCATTAAGTGATGCTGAACGCTTTATCGAAGAAGGTCATTTTGCGAAAGGTTCTATGCTGCCTAAAGTGGAAGCTGCCGCCTCGTTTGCTCGCTCTCGCGCAGGTCGTGAAGCACTGATTACCGTATTGAGCAAAGCGAAAGAAGGGATTGAAGGCAAAACAGGAACGGTGATTTGT > NC_000913/3012126‑3012387
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
