Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,145,871 |
T→C |
M131V (ATG→GTG) |
hybO ← |
hydrogenase 2, small subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,145,871 | 0 | T | C | 100.0%
| 152.1
/ NA
| 42 | M131V (ATG→GTG) | hybO | hydrogenase 2, small subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/22); total (20/22) |
ACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATA > NC_000913/3145741‑3146006
|
aCTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCaataaa < 2:877626/139‑1 (MQ=255)
aCTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCaataaa > 1:877626/1‑139 (MQ=255)
cAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGtt < 2:513660/139‑1 (MQ=255)
cAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGtt < 1:506516/139‑1 (MQ=255)
ttGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATc > 2:612964/1‑139 (MQ=255)
aGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGc < 2:887398/110‑1 (MQ=255)
aGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGc > 1:887398/1‑110 (MQ=255)
gCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCAt > 2:751776/1‑139 (MQ=255)
cAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATc < 1:612964/139‑1 (MQ=255)
ccGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATccacca < 1:516825/139‑1 (MQ=255)
ccGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATccacca < 1:818653/139‑1 (MQ=255)
ccACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAac < 2:886705/139‑1 (MQ=255)
cGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGtt > 1:690959/1‑102 (MQ=255)
cGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGtt < 2:690959/102‑1 (MQ=255)
gAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCtt > 1:847842/1‑139 (MQ=255)
aCCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTg > 1:500422/1‑139 (MQ=255)
cGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTa < 1:751776/139‑1 (MQ=255)
gATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTAc > 2:503875/1‑139 (MQ=255)
aTAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATg < 1:601231/96‑1 (MQ=255)
aTAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATg > 2:601231/1‑96 (MQ=255)
tCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTg < 2:676002/111‑1 (MQ=255)
tCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTg > 1:676002/1‑111 (MQ=255)
ccgccgCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATcc < 1:803019/86‑1 (MQ=255)
ccgccgCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATcc > 2:803019/1‑86 (MQ=255)
cgccgcTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCg < 1:240429/58‑1 (MQ=255)
cgccgcTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCg > 2:240429/1‑58 (MQ=255)
ccgcTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTc < 1:503875/139‑1 (MQ=255)
cGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATcc > 1:853822/1‑75 (MQ=255)
cGGATGTGATCCACAATCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATcc < 2:853822/75‑1 (MQ=255)
aTCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATccac > 2:594828/1‑62 (MQ=255)
aTCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATccac < 1:594828/62‑1 (MQ=255)
tCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTaa > 1:235989/1‑43 (MQ=255)
tCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTaa < 2:235989/43‑1 (MQ=255)
tCGGCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGa > 1:727331/1‑139 (MQ=255)
gCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAg < 2:177565/139‑1 (MQ=255)
gCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAg > 1:498957/1‑139 (MQ=255)
gCTCACCGGCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAg > 2:826564/1‑139 (MQ=255)
ggCAACCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCg < 2:498957/139‑1 (MQ=255)
aCCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACtt > 1:437962/1‑133 (MQ=255)
aCCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACtt < 2:437962/133‑1 (MQ=255)
aCCACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGAt > 2:889777/1‑139 (MQ=255)
ccACGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATa < 1:826564/139‑1 (MQ=255)
|
ACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATA > NC_000913/3145741‑3146006
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
