Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A10 F1 I1 R1 | 759 | 65.2 | 3386260 | 90.7% | 3071337 | 101.6 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,472,207 | G→A | G436G (GGC→GGT) | fusA ← | protein chain elongation factor EF‑G, GTP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,472,207 | 0 | G | A | 100.0% | 198.6 / NA | 61 | G436G (GGC→GGT) | fusA | protein chain elongation factor EF‑G, GTP‑binding |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/33); total (28/33) | |||||||||||
TCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGGCCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCGGCGCATCCGGGTCACACAGGGTGTCACCA > NC_000913/3472071‑3472336 | tCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGAcc < 2:1028812/139‑1 (MQ=255) tttCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGACGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCATTAGCCAGACGACCCag > 1:1040579/2‑139 (MQ=255) cAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGc < 1:596066/139‑1 (MQ=255) aaTTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGAc < 2:343783/136‑1 (MQ=255) aaTTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGAc > 1:343783/1‑136 (MQ=255) aTACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGt > 1:173238/1‑139 (MQ=255) gTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGGCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCt > 1:910650/1‑139 (MQ=255) tgTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCgg > 2:788196/1‑139 (MQ=255) gTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGt < 2:599699/139‑1 (MQ=255) aGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCaa < 2:1040579/139‑1 (MQ=255) ggTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAAc < 1:788196/139‑1 (MQ=255) gTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGAcc < 1:29196/97‑1 (MQ=255) gTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGAcc > 2:29196/1‑97 (MQ=255) cGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGa < 1:241482/135‑1 (MQ=255) cGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGa > 2:241482/1‑135 (MQ=255) aTACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTa < 2:173238/139‑1 (MQ=255) tACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTAc < 1:411568/139‑1 (MQ=255) cgcgATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGtt > 2:1510642/1‑109 (MQ=255) cgcgATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGtt < 1:1510642/109‑1 (MQ=255) ggTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAAt > 2:254961/1‑139 (MQ=255) tCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTc > 2:1475051/1‑139 (MQ=255) ggTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCAt < 1:333696/139‑1 (MQ=255) gTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTgg > 1:644477/1‑82 (MQ=255) gTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTgg < 2:327527/82‑1 (MQ=255) gTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTgg > 1:327527/1‑82 (MQ=255) gTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTgg < 2:644477/82‑1 (MQ=255) gTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATa < 2:910650/139‑1 (MQ=255) ttcttcGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGAccc > 2:277437/1‑52 (MQ=255) ttcttcGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGAccc < 1:277437/52‑1 (MQ=255) tcGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGaa > 1:447569/1‑139 (MQ=255) gTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCTGCTTTGGtttt < 1:621238/84‑1 (MQ=255) gTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCTGCTTTGGtttt > 2:621238/1‑84 (MQ=255) tCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTAc < 1:1563711/103‑1 (MQ=255) tCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTAc > 2:1563711/1‑103 (MQ=255) tCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAgg < 2:242675/112‑1 (MQ=255) tCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAgg > 1:242675/1‑112 (MQ=255) tCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATg < 1:545218/134‑1 (MQ=255) tCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATg > 2:545218/1‑134 (MQ=255) acacGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCgg > 2:926804/1‑134 (MQ=255) acacGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCgg < 1:926804/134‑1 (MQ=255) cacGGAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCGGCGCATc < 2:447569/139‑1 (MQ=255) ggAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCGGCGCATCCgg > 1:227855/1‑139 (MQ=255) gAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCt > 1:397298/1‑61 (MQ=255) gAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCt < 2:397298/61‑1 (MQ=255) gAAAGACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCGGCGCATCCggg > 1:503759/1‑139 (MQ=255) gACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGa < 2:949544/79‑1 (MQ=255) gACGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGa > 1:949544/1‑79 (MQ=255) aCGGGTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCGGCGCATCCGGGTcaca > 1:871930/1‑139 (MQ=255) gTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTc > 2:917787/1‑66 (MQ=255) gTCTTCTTTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTc < 1:917787/66‑1 (MQ=255) cttcttTAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCGGCGCATCCGGGTCACACAGGgt < 1:254961/139‑1 (MQ=255) tAGCCGGACGACCCAGACTCAGACCCATTGTTTCCTGGTCAGCTTTGTTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCGGCGCAACCGGGTCACGCAGGGTGTCAcc < 2:1371597/139‑1 (MQ=255) tAGCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCGGCGCATCCGGGTCACACAGGGTGTCAcc < 2:871930/139‑1 (MQ=255) gCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATgg < 1:1377160/67‑1 (MQ=255) gCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATgg > 2:1377160/1‑67 (MQ=255) gCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAggg > 1:1120258/1‑84 (MQ=255) gCCAGACGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAggg < 2:1120258/84‑1 (MQ=255) aCGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGtt < 2:238594/43‑1 (MQ=255) aCGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCGGCGCAt < 2:148970/110‑1 (MQ=255) aCGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCGGCGCAt > 1:148970/1‑110 (MQ=255) aCGACCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCGGCGCATCCGGGTCACACAGGGTGTCACCa < 2:1028488/133‑1 (MQ=255) | TCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTCAGTCCATACACGGAAAGACGGGTCTTCTTTAGCCAGACGGCCCAGAGCCAGACCCATTTTTTCCTGGTCAGCTTTGGTTTTCGGTTCAACTGCGATGGAGATTACCGGCTCAGGGAATTCCATACGTTCCAGAATGATCGGCGCATCCGGGTCACACAGGGTGTCACCA > NC_000913/3472071‑3472336 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |