Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
759 |
65.2 |
3386260 |
90.7% |
3071337 |
101.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,567,251 |
C→T |
L261L (TTG→TTA) |
glgA ← |
glycogen synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,567,251 | 0 | C | T | 100.0%
| 137.6
/ NA
| 43 | L261L (TTG→TTA) | glgA | glycogen synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (25/18); total (25/18) |
ACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCG > NC_000913/3567144‑3567385
|
acTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAg < 2:1692488/117‑1 (MQ=255)
acTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAg > 1:1692488/1‑117 (MQ=255)
cAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTtctc > 1:292675/1‑139 (MQ=255)
cAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTtctc > 2:387149/1‑139 (MQ=255)
aGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGAc < 2:292675/139‑1 (MQ=255)
cATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGt < 1:549971/107‑1 (MQ=255)
cATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGt > 2:549971/1‑107 (MQ=255)
ttGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTc > 2:236792/1‑139 (MQ=255)
aTTTGTAACTGGCTCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGt < 1:236792/139‑1 (MQ=255)
gcgcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGt > 1:1190345/1‑128 (MQ=255)
gcgcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGt < 2:1190345/128‑1 (MQ=255)
gcgcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGc > 2:110480/1‑139 (MQ=255)
gcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCaa > 1:835629/1‑139 (MQ=255)
tATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg < 2:1106570/139‑1 (MQ=255)
ttttCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGAc < 2:835629/139‑1 (MQ=255)
cAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAg > 2:853397/1‑90 (MQ=255)
cAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAg < 1:853397/90‑1 (MQ=255)
cGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGt < 2:1116256/62‑1 (MQ=255)
cGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGt > 1:1116256/1‑62 (MQ=255)
cGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAAc > 2:645549/1‑139 (MQ=255)
cGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACCCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGt < 2:245833/101‑1 (MQ=255)
cGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACCCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGt > 1:245833/1‑101 (MQ=255)
tATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGtt > 1:1619746/1‑70 (MQ=255)
tATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGtt < 2:1619746/70‑1 (MQ=255)
tATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTg > 2:99922/1‑139 (MQ=255)
tCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGAc > 2:371731/1‑43 (MQ=255)
tCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGAc < 1:371731/43‑1 (MQ=255)
tCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGt < 2:1127164/97‑1 (MQ=255)
tCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGt > 1:1127164/1‑97 (MQ=255)
tCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACg < 1:457982/101‑1 (MQ=255)
tCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACg > 2:457982/1‑101 (MQ=255)
tCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCg > 1:1530112/1‑139 (MQ=255)
tCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCg > 1:1385819/1‑139 (MQ=255)
ggTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCg > 2:712933/1‑139 (MQ=255)
tAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCaaa > 1:1292494/1‑125 (MQ=255)
tAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCaaa < 2:1292494/125‑1 (MQ=255)
aCGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCtt > 2:1098078/1‑107 (MQ=255)
aCGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCtt < 1:1098078/107‑1 (MQ=255)
aGGCTAACAGTAAGTCCGTCTCTGGACTCCAGAtttt > 2:1378988/1‑37 (MQ=255)
aGGCTAACAGTAAGTCCGTCTCTGGACTCCAGAtttt < 1:1378988/37‑1 (MQ=255)
aGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcgcg > 2:602231/1‑139 (MQ=255)
aGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcgcg > 2:912971/1‑139 (MQ=255)
ggCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGc < 2:969340/85‑1 (MQ=255)
|
ACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCG > NC_000913/3567144‑3567385
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
