Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I1 R1 781 46.9 2231650 93.5% 2086592 109.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,905,219 (C)6→7 coding (200/672 nt) queE ← 7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,905,2131.C100.0% 171.6 / NA 48A69G (GCT→GGT) queE7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (24/24);  total (24/24)

AGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAA  >  NC_000913/2905076‑2905345
                                                                                                                                          |                                                                                                                                    
aGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑c                                                                                                                                     <  1:161340/139‑1 (MQ=255)
aGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑c                                                                                                                                     <  2:1040510/139‑1 (MQ=255)
    aaaaCCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑ccccc                                                                                                                                 <  1:173303/139‑1 (MQ=255)
             cTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATc                                                                                                                         >  2:22943/1‑139 (MQ=255)
                ttCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAct                                                                                                                      <  1:377846/139‑1 (MQ=255)
                ttCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAct                                                                                                                      <  1:59424/139‑1 (MQ=255)
                          tcagtcagTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTc                                                                                                            <  1:432419/139‑1 (MQ=255)
                                   ggCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCTAATCTTCACTGCTCGCAGCCCCCCCACTTATCGCTCGCTTTGGTCTTCGCCAGa                                                                                                   <  2:746121/139‑1 (MQ=255)
                                   ggCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAc                                                                                                                       >  1:256903/1‑119 (MQ=255)
                                   ggCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAc                                                                                                                       <  2:256903/119‑1 (MQ=255)
                                                  aTGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGgag                                                                                    <  1:988553/139‑1 (MQ=255)
                                                   tGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCtt                                                                                                          <  1:693198/116‑1 (MQ=255)
                                                   ggCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCtt                                                                                                          >  2:693198/2‑116 (MQ=255)
                                                         gCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑ccc                                                                                                                                   >  1:945256/1‑84 (MQ=255)
                                                         gCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑ccc                                                                                                                                   <  2:945256/84‑1 (MQ=255)
                                                            caccacCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAg                                                                                                                                       <  2:935267/78‑1 (MQ=255)
                                                            caccacCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAg                                                                                                                                       >  1:935267/1‑78 (MQ=255)
                                                                accCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTc                                                                      <  2:930521/139‑1 (MQ=255)
                                                                  ccGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCaa                                                                    >  1:437321/1‑139 (MQ=255)
                                                                     tAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGgaga                                                                                   >  2:93276/1‑121 (MQ=255)
                                                                     tAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGgaga                                                                                   <  1:93276/121‑1 (MQ=255)
                                                                     tAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCt                                                                 <  2:437321/139‑1 (MQ=255)
                                                                               aTGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgt                                                       >  2:761049/1‑139 (MQ=255)
                                                                                 gCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgtgt                                                     >  2:208842/1‑139 (MQ=255)
                                                                                   cgcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTg                                                                                                               >  2:955181/1‑79 (MQ=255)
                                                                                   cgcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTg                                                                                                               <  1:955181/79‑1 (MQ=255)
                                                                                   cgcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgtgtgt                                                   >  1:351236/1‑139 (MQ=255)
                                                                                    gcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGtt                                                  <  1:727054/139‑1 (MQ=255)
                                                                                     cgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGttt                                                 >  2:317940/1‑139 (MQ=255)
                                                                                               cccTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCAc                                       <  1:317940/139‑1 (MQ=255)
                                                                                                    gCGACCAATGACAGTCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTg                                                                                             <  2:758909/80‑1 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAc                                                                                                                       <  2:757652/54‑1 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAc                                                                                                                       >  1:757652/1‑54 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAAt                                                                                                 <  2:59490/76‑1 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAAt                                                                                                 >  1:59490/1‑76 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTg                                                                                             >  1:758909/1‑80 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTcc                                                            <  2:1072989/113‑1 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTcc                                                            >  1:1072989/1‑113 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgt                                                       >  2:151951/1‑118 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgt                                                       <  1:151951/118‑1 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGc                                  >  1:746248/1‑139 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGc                                  >  2:142327/1‑139 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGc                                  >  2:519015/1‑139 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGc                                  >  2:631304/1‑139 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGc                                  >  2:632863/1‑139 (MQ=255)
                                                                                                    gCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGc                                  >  2:694106/1‑139 (MQ=255)
                                                                                                            tGACAGCCAGCAAAGCTTCACTGGTCGCAGCCCCCCCACTTATCTCTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCGAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCaa                          <  2:619233/139‑1 (MQ=255)
                                                                                                                 gccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCggg                     <  1:631304/139‑1 (MQ=255)
                                                                                                                  ccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGgag                                                                                    <  1:541197/75‑1 (MQ=255)
                                                                                                                  ccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGgag                                                                                    >  2:541197/1‑75 (MQ=255)
                                                                                                                   cagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCa                   <  1:761049/139‑1 (MQ=255)
                                                                                                                    agcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCAt                  <  1:22943/139‑1 (MQ=255)
                                                                                                                                   cTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAg                                                                   >  2:88251/1‑75 (MQ=255)
                                                                                                                                   cTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAg                                                                   <  1:88251/75‑1 (MQ=255)
                                                                                                                                    tCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATaa  >  2:25990/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                    
AGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAA  >  NC_000913/2905076‑2905345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A