Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
781 |
46.9 |
2231650 |
93.5% |
2086592 |
109.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,079,798 |
G→A |
A613V (GCG→GTG) |
tktA ← |
transketolase 1, thiamine triphosphate‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,079,798 | 0 | G | A | 100.0%
| 144.4
/ NA
| 45 | A613V (GCG→GTG) | tktA | transketolase 1, thiamine triphosphate‑binding |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/20); total (25/20) |
CGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTC > NC_000913/3079665‑3079931
|
cGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCt > 1:471987/1‑139 (MQ=255)
tATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAAcgcg > 2:913000/1‑139 (MQ=255)
aGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTa > 1:1009063/1‑139 (MQ=255)
aaGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATAGTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTGACCg > 1:734296/1‑139 (MQ=255)
aGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGc > 2:353627/1‑139 (MQ=255)
aCTCTTCAAACTGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCgg < 1:722982/139‑1 (MQ=255)
ctctGCCGGAGCAGATTCACCGGAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTg < 1:157314/107‑1 (MQ=255)
ctctGCCGGAGCAGATTCACCGGAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTg > 2:157314/1‑107 (MQ=255)
tctGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAAcgcg < 1:1063716/104‑1 (MQ=255)
tctGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAAcgcg > 2:1063716/1‑104 (MQ=255)
tctGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGt < 1:913000/139‑1 (MQ=255)
ggAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagcag < 1:1054596/139‑1 (MQ=255)
gtggtCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATg < 1:286264/139‑1 (MQ=255)
ccGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAg < 1:7061/139‑1 (MQ=255)
cgaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGTAAcgcg < 2:54125/67‑1 (MQ=37)
cgaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGTAAcgcg > 1:54125/1‑67 (MQ=255)
gaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGc > 2:75540/1‑139 (MQ=255)
cACCGTTCAGGCCTACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGTAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGa < 2:734296/139‑1 (MQ=255)
ttCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcacca > 1:1037240/1‑139 (MQ=255)
cATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTg > 1:418613/1‑48 (MQ=255)
cATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTg < 2:418613/48‑1 (MQ=255)
cATACTTGTACCAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCttt > 1:852827/1‑139 (MQ=255)
ccAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAc > 2:407367/1‑106 (MQ=255)
ccAGTAGTCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAc < 1:407367/106‑1 (MQ=255)
gtagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcgcgc > 2:292418/1‑121 (MQ=255)
gtagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcgcgc < 1:292418/121‑1 (MQ=255)
gtagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGca > 2:495966/1‑139 (MQ=255)
gtagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGca > 1:1036933/1‑139 (MQ=255)
tagtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcag < 2:724001/139‑1 (MQ=255)
agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACg > 1:844694/1‑56 (MQ=255)
agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACg < 2:844694/56‑1 (MQ=255)
agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAc < 2:99306/101‑1 (MQ=255)
agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAc > 1:99306/1‑101 (MQ=255)
agtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagt < 2:1036933/139‑1 (MQ=255)
gtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGa < 2:258526/99‑1 (MQ=255)
gtCAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGa > 1:258526/1‑99 (MQ=255)
cAGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcag > 2:398251/1‑139 (MQ=255)
aGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcagt > 2:600427/1‑139 (MQ=255)
aGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcagt > 2:698706/1‑139 (MQ=255)
aGCAATACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcagt < 2:1009063/139‑1 (MQ=255)
tACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagt > 1:793480/1‑130 (MQ=255)
tACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagt < 2:793480/130‑1 (MQ=255)
tACCCACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTc > 1:240595/1‑139 (MQ=255)
cccACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAg > 2:434443/1‑41 (MQ=255)
cccACTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAg < 1:434443/41‑1 (MQ=255)
|
CGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTC > NC_000913/3079665‑3079931
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
