Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,084,031	T→C	Q627Q (CAA→CAG)	speA ←	biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,084,031	0	T	C	100.0%	150.9 / NA	42	Q627Q (CAA→CAG)	speA	biosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/20); total (22/20)

GCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGC  >  NC_000913/3083903‑3084162

gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGa                                                                                                                           <  2:529221/139‑1 (MQ=255)

  tcaaTTAATCGGAATCAAGTAACGTAGCAGCTTCTCATCTTCAATATAGGAATACTTGTACAAACCTGCCTCGAACTCTTGAAGGAACTGTTGTGGCAGTCCAGCATCAATTTCGGTTTTCTTCACCTGATCGCGGAAc                                                                                                                         <  2:486409/137‑1 (MQ=255)

         aGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTa                                                                                                                  >  1:905306/1‑139 (MQ=255)

              aTTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTACAACCGTACAAACCTGCCTCGAACTCTTCAAGGGACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGa                                                                                                                           >  1:118778/1‑125 (MQ=255)

              aTTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTACAACCGTACAAACCTGCCTCGAACTCTTCAAGGGACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGa                                                                                                                           <  2:118778/125‑1 (MQ=255)

                         acaGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATc                                                                                                  <  2:788926/139‑1 (MQ=255)

                            ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               <  2:331693/139‑1 (MQ=255)

                            ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               >  2:450098/1‑139 (MQ=255)

                            ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               >  2:615531/1‑139 (MQ=255)

                                     cATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                                                                                             <  2:137738/132‑1 (MQ=255)

                                     cATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                                                                                             >  1:137738/1‑132 (MQ=255)

                                                        aCCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTAc                                                                                         <  2:969143/117‑1 (MQ=255)

                                                        aCCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTAc                                                                                         >  1:969143/1‑117 (MQ=255)

                                                         ccGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACgg                                                                  >  2:652477/1‑139 (MQ=255)

                                                            tACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               <  2:500744/107‑1 (MQ=255)

                                                            tACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               >  1:500744/1‑107 (MQ=255)

                                                            tACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGcc                                                                      >  1:300176/1‑132 (MQ=255)

                                                            tACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGcc                                                                      <  2:300176/132‑1 (MQ=255)

                                                            tACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTAt                                                               >  2:1011715/1‑139 (MQ=255)

                                                             aCAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               <  2:357488/106‑1 (MQ=255)

                                                             aCAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAg                                                                                               >  1:357488/1‑106 (MQ=255)

                                                              cAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAGGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                                                                                             >  2:538689/1‑107 (MQ=255)

                                                              cAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAGGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                                                                                             <  1:538689/107‑1 (MQ=255)

                                                              cAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCg                                                             >  2:1084279/1‑139 (MQ=255)

                                                              cAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCg                                                             >  1:625068/1‑139 (MQ=255)

                                                                   cTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                                                                                             >  1:893455/1‑102 (MQ=255)

                                                                   cTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCt                                                                                             <  2:893455/102‑1 (MQ=255)

                                                                         cGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGa                                                  <  2:905306/139‑1 (MQ=255)

                                                                         cGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGa                                                  <  1:652477/139‑1 (MQ=255)

                                                                                ttCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGttct                                           >  2:974431/1‑139 (MQ=255)

                                                                                ttCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGttct                                           <  1:149138/139‑1 (MQ=255)

                                                                                ttCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGttct                                           >  1:864316/1‑139 (MQ=255)

                                                                                      gAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGttctacttct                                     <  1:450098/139‑1 (MQ=255)

                                                                                        aCTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGAc                                                 <  2:862865/125‑1 (MQ=255)

                                                                                        aCTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGAc                                                 >  1:862865/1‑125 (MQ=255)

                                                                                          tgttgtTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGc                                 >  2:582045/1‑139 (MQ=255)

                                                                                          tgttgtTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGc                                 >  1:730581/1‑139 (MQ=255)

                                                                                          tgttgtTGCAGTTCAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGc                                 >  1:1029472/1‑139 (MQ=255)

                                                                                           gttgttGCAGTACAGCATCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCt                                <  1:720609/139‑1 (MQ=255)

                                                                                                           aTCAAGATCGGTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACg                <  1:582045/139‑1 (MQ=255)

                                                                                                                    ggTTTTCTTCACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCa       >  2:863004/1‑139 (MQ=255)

                                                                                                                         tcttcACCTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGc  <  2:1029472/139‑1 (MQ=255)

GCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCAGCATCAAGATCGGTTTTCTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGC  >  NC_000913/3083903‑3084162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A