Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,830,265	T→C	S441P (TCC→CCC)	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,830,265	0	T	C	100.0%	127.1 / NA	36	S441P (TCC→CCC)	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/20); total (16/20)

CTCGTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCC  >  NC_000913/3830143‑3830392

ctcGTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATcgccgc                                                                                                                 <  2:990221/139‑1 (MQ=255)

   gTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCgcggg                                                                                                              <  1:1032832/139‑1 (MQ=255)

   gTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCgcggg                                                                                                              >  1:191479/1‑139 (MQ=255)

   gTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCgcggg                                                                                                              >  2:139950/1‑139 (MQ=255)

       gCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGt                                                                                                          <  1:266026/139‑1 (MQ=255)

        ccGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTa                                                                                                         >  2:894862/1‑139 (MQ=255)

         cGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTAt                                                                                                        <  1:402994/139‑1 (MQ=255)

           cTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTa                                                                                                      >  1:1044776/1‑139 (MQ=255)

                 cGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCa                                                                                                <  2:141289/139‑1 (MQ=255)

                      ggCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTg                                                                                           <  1:930204/139‑1 (MQ=255)

                      ggCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTg                                                                                           <  1:894862/139‑1 (MQ=255)

                          aTTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCa                                                                                                <  2:738670/130‑1 (MQ=255)

                          aTTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCa                                                                                                >  1:738670/1‑130 (MQ=255)

                            tCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTATGCCCCTGAATGGCTGAAAAAACTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAAt                                                                                     <  1:647270/139‑1 (MQ=255)

                                aTTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACCGCCATCGTGCTGAATCTGa                                                                                 <  1:139950/139‑1 (MQ=255)

                                aTTATCGCGCTGTCGCTGGCGCTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTACCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGa                                                                                 >  2:635189/1‑139 (MQ=255)

                                       cgcTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTccc                                                                          >  1:133848/1‑139 (MQ=255)

                                         cTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCccac                                                                        <  2:15984/139‑1 (MQ=255)

                                                                    tctcAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGgcggcg                                             >  2:469718/1‑139 (MQ=255)

                                                                        agcagcCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTaa                                         >  2:684229/1‑139 (MQ=255)

                                                                         gcagcCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAg                                        <  2:1044776/139‑1 (MQ=255)

                                                                             ccGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATaa                                    >  1:967608/1‑139 (MQ=255)

                                                                             ccGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATaa                                    >  1:344951/1‑139 (MQ=255)

                                                                             ccGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATaa                                    >  2:402254/1‑139 (MQ=255)

                                                                                     tttGCAGTTTGACCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTAccgcc                            <  1:684229/139‑1 (MQ=255)

                                                                                             ttGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCCGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGtctc                    >  2:553660/1‑139 (MQ=255)

                                                                                              tGCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCa                   <  2:750033/139‑1 (MQ=255)

                                                                                               gCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCAc                  <  1:613985/139‑1 (MQ=255)

                                                                                               gCCCCTGAATGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCAc                  <  1:553660/139‑1 (MQ=255)

                                                                                                      aaTGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCAc                  <  1:777090/132‑1 (MQ=255)

                                                                                                      aaTGGCTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCAc                  >  2:777090/1‑132 (MQ=255)

                                                                                                           cTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCAc                  >  2:484151/1‑127 (MQ=255)

                                                                                                           cTGAAAAACCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCAc                  <  1:484151/127‑1 (MQ=255)

                                                                                                               aaaaCCTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATCACCGCCGCAGTCTCACCTCTTTAACCATTccc  <  1:469718/139‑1 (MQ=255)

                                                                                                                    cTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTccc                                                                          >  2:364046/1‑62 (MQ=255)

                                                                                                                    cTGCTCCCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTccc                                                                          <  1:364046/62‑1 (MQ=255)

CTCGTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCAGCAGCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCC  >  NC_000913/3830143‑3830392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A