Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,950,420	G→A	33 (TAG→TAA)	ilvL →	ilvG operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,950,420	0	G	A	100.0%	141.5 / NA	44	33 (TAG→TAA)	ilvL	ilvG operon leader peptide
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/21); total (23/21)

TTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAAC  >  NC_000913/3950291‑3950554

ttCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGc                                                                                                                               >  2:300948/1‑139 (MQ=255)

                  aaGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGAccc                                                                                                             <  1:782347/139‑1 (MQ=255)

                    gAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCACACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGAccccc                                                                                                           <  2:617261/139‑1 (MQ=255)

                            aaaaTGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaa                                                                                                   <  1:778282/139‑1 (MQ=255)

                                               gAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtttttttt                                                                                <  2:469883/139‑1 (MQ=255)

                                                 gTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGa                                                                              >  1:351137/1‑139 (MQ=255)

                                                    aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGc                                                                                                         >  2:61837/1‑109 (MQ=255)

                                                    aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGc                                                                                                         <  1:61837/109‑1 (MQ=255)

                                                    aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCt                                                                           >  2:328094/1‑139 (MQ=255)

                                                    aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCt                                                                           >  1:980835/1‑139 (MQ=255)

                                                     ttAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCtt                                                                          >  2:522715/1‑139 (MQ=255)

                                                         ccTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGAAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTaaaa                                                                      >  2:689753/1‑139 (MQ=255)

                                                              tCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATa                                                                 >  2:462820/1‑139 (MQ=255)

                                                                       gCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCgaggag                                                        >  2:49158/1‑139 (MQ=255)

                                                                         gtggtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                      <  2:100061/139‑1 (MQ=255)

                                                                           ggtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                    <  1:328094/139‑1 (MQ=255)

                                                                               gtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGc                                                       >  1:754990/1‑132 (MQ=255)

                                                                               gtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGc                                                       <  2:754990/132‑1 (MQ=255)

                                                                                  attattATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGa                                                                              >  1:641238/1‑106 (MQ=255)

                                                                                  attattATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGa                                                                              <  2:641238/106‑1 (MQ=255)

                                                                                       tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa                                        >  1:7136/1‑139 (MQ=255)

                                                                                       tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa                                        >  2:852920/1‑139 (MQ=255)

                                                                                            cACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcaca                                   >  1:1004799/1‑139 (MQ=255)

                                                                                              ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtttttttt                                                                                >  1:832749/1‑92 (MQ=255)

                                                                                              ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtttttttt                                                                                <  2:832749/92‑1 (MQ=255)

                                                                                              ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACaa                                                 >  2:872959/1‑123 (MQ=255)

                                                                                              ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACaa                                                 <  1:872959/123‑1 (MQ=255)

                                                                                              ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaa                                 >  1:205461/1‑139 (MQ=255)

                                                                                                   cGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTaa                                                                                                                  <  1:771334/53‑1 (MQ=255)

                                                                                                   cGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTaa                                                                                                                  >  2:771334/1‑53 (MQ=255)

                                                                                                      ggCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTa                                                                                                                           <  1:92023/41‑1 (MQ=255)

                                                                                                      ggCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTa                                                                                                                           >  2:92023/1‑41 (MQ=255)

                                                                                                         tGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct                      >  2:976301/1‑139 (MQ=255)

                                                                                                           cACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa                    <  1:852920/139‑1 (MQ=255)

                                                                                                           cACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa                    <  1:300948/139‑1 (MQ=255)

                                                                                                                ggACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAtt               >  2:837503/1‑139 (MQ=255)

                                                                                                                     aGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGtt                         >  2:257768/1‑124 (MQ=255)

                                                                                                                     aGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGtt                         <  1:257768/124‑1 (MQ=255)

                                                                                                                      ggAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGAc         <  1:602423/139‑1 (MQ=255)

                                                                                                                        aaaGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACgg       <  2:351137/139‑1 (MQ=255)

                                                                                                                        aaaGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACgg       <  2:7136/139‑1 (MQ=255)

                                                                                                                            gCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                    <  2:194855/90‑1 (MQ=255)

                                                                                                                            gCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                    >  1:194855/1‑90 (MQ=255)

                                                                                                                             cTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac  <  1:522715/139‑1 (MQ=255)

TTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAAC  >  NC_000913/3950291‑3950554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A