Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,999,821 |
G→A |
A24V (GCG→GTG) |
ygeR ← |
LysM domain‑containing M23 family putative peptidase, septation lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,999,821 | 0 | G | A | 93.9%
| 184.7
/ 3.3
| 66 | A24V (GCG→GTG) | ygeR | LysM domain‑containing M23 family putative peptidase, septation lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (28/34); total (30/36) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01 |
TCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTG > NC_000913/2999690‑2999944
|
tCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAg < 2:906037/139‑1 (MQ=255)
cgcgCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTc < 2:253439/139‑1 (MQ=255)
cTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaa < 1:1257794/139‑1 (MQ=255)
ttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaacaa < 2:444589/139‑1 (MQ=255)
gTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAg > 1:817067/1‑113 (MQ=255)
gTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAg < 2:817067/113‑1 (MQ=255)
gTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaac > 1:917912/1‑129 (MQ=255)
gTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGCAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaac < 2:917912/129‑1 (MQ=255)
ggtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCaga < 1:1080255/139‑1 (MQ=255)
gtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCagag < 2:561164/139‑1 (MQ=255)
gtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCagag > 2:202743/1‑139 (MQ=255)
tgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAgaga > 1:654698/1‑139 (MQ=255)
cgcgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATaa < 2:453710/116‑1 (MQ=255)
cgcgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATaa > 1:453710/1‑116 (MQ=255)
aGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaa < 2:351482/101‑1 (MQ=255)
aGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaa > 1:351482/1‑101 (MQ=255)
tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGcccccc > 1:899534/1‑77 (MQ=255)
tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCAcc < 2:899534/77‑1 (MQ=255)
tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCagag > 2:1310203/1‑117 (MQ=255)
tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCagag < 1:1310203/117‑1 (MQ=255)
tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGc > 2:848863/1‑139 (MQ=255)
ccccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACt > 1:527704/1‑139 (MQ=255)
ccccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCa < 1:1010085/86‑1 (MQ=255)
ccccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCa > 2:1010085/1‑86 (MQ=255)
ccccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaacaa < 2:1311850/97‑1 (MQ=255)
ccccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaacaa > 1:1311850/1‑97 (MQ=255)
ccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCa < 1:202743/139‑1 (MQ=255)
ccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTc > 1:992713/1‑80 (MQ=255)
ccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTc < 2:992713/80‑1 (MQ=255)
tCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCtt > 2:821889/1‑139 (MQ=255)
gtgtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTc < 2:654698/139‑1 (MQ=255)
gtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACGCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGc > 2:950481/1‑139 (MQ=255)
gtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGc > 1:1112432/1‑139 (MQ=255)
gCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaacaa > 1:13778/1‑72 (MQ=255)
gCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaacaa < 2:13778/72‑1 (MQ=255)
gCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCagag < 1:1129630/89‑1 (MQ=255)
gCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCagag > 2:1129630/1‑89 (MQ=255)
gCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAAc > 2:1104598/1‑139 (MQ=255)
cGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACaa > 2:353672/1‑139 (MQ=255)
cGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACaa > 2:348698/1‑139 (MQ=255)
gAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGa < 1:950481/139‑1 (MQ=255)
gAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGa < 1:1104598/139‑1 (MQ=255)
gAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGa < 2:1112432/139‑1 (MQ=255)
aaTACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCaga > 1:1244754/1‑83 (MQ=255)
aaTACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCaga < 2:1244754/83‑1 (MQ=255)
aTACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACg < 1:1164626/139‑1 (MQ=255)
tACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGc < 2:527704/139‑1 (MQ=255)
tACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGc < 1:353672/139‑1 (MQ=255)
tACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCATTCCTCGCTTAAAAAAGAAGACGc < 1:821889/139‑1 (MQ=255)
tCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagca > 1:236722/1‑139 (MQ=255)
gatgatTTGCTACCCGAACAGCCCACCAAAAGCAGTc < 1:33191/37‑1 (MQ=255)
gatgatTTGCTACCCGAACAGCCCACCAAAAGCAGTc > 2:33191/1‑37 (MQ=255)
gatgatTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGc < 2:221246/82‑1 (MQ=255)
gatgatTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGc > 1:221246/1‑82 (MQ=255)
gatgatTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGcagcc > 2:918978/1‑139 (MQ=255)
tgatTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATATTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGcagccag > 2:663956/1‑139 (MQ=255)
gatTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGcagccagc < 1:918978/139‑1 (MQ=255)
atTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCagccagcc < 2:236722/139‑1 (MQ=255)
tttGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGcagccagc > 2:1130747/1‑137 (MQ=255)
tttGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGcagccagc < 1:1130747/137‑1 (MQ=255)
cTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTg < 1:663956/139‑1 (MQ=255)
aCCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCagccagcc < 2:762763/132‑1 (MQ=255)
aCCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCagccagcc > 1:762763/1‑132 (MQ=255)
gAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCg > 2:526235/1‑73 (MQ=255)
gAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCg < 1:526235/73‑1 (MQ=255)
gCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCa < 1:191007/44‑1 (MQ=255)
gCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCa > 2:191007/1‑44 (MQ=255)
|
TCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTG > NC_000913/2999690‑2999944
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
