Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,161,578	A→G	I364I (ATT→ATC)	ftsP ←	septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,161,578	0	A	G	100.0%	183.9 / NA	51	I364I (ATT→ATC)	ftsP	septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/25); total (26/25)

ACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCA  >  NC_000913/3161452‑3161711

aCGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGtca                                                                                                                           >  2:405538/1‑139 (MQ=255)

aCGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGtca                                                                                                                           <  2:1772731/139‑1 (MQ=255)

       tGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTTGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATAcc                                                                                                                                  >  1:1612363/1‑125 (MQ=255)

       tGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTTGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATAcc                                                                                                                                  <  2:1612363/125‑1 (MQ=255)

       tGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATAccc                                                                                                                                 <  1:619657/126‑1 (MQ=255)

       tGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATAccc                                                                                                                                 >  2:619657/1‑126 (MQ=255)

           aCATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACt                                                                                                                <  2:1582082/139‑1 (MQ=255)

                 cGCCTTCAATATGGAACGCATGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATc                                                                                                          >  1:253483/1‑139 (MQ=255)

                              gAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAAtt                                                                                             >  2:180672/1‑139 (MQ=255)

                                 cGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGc                                                                                          >  1:1507544/1‑139 (MQ=255)

                                  gCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGtc                                                                                                                            >  1:241901/1‑104 (MQ=255)

                                  gCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGtc                                                                                                                            <  2:241901/104‑1 (MQ=255)

                                    ttGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGaa                                                                                       >  2:1786334/1‑139 (MQ=255)

                                    ttGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGaa                                                                                       >  1:497944/1‑139 (MQ=255)

                                      gCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCAc                                                                                                                       >  2:1736455/1‑105 (MQ=255)

                                      gCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCAc                                                                                                                       <  1:1736455/105‑1 (MQ=255)

                                      gCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAAcc                                                                                     >  2:1251382/1‑139 (MQ=255)

                                         gCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGc                                                                                  <  2:1113289/139‑1 (MQ=255)

                                                               gCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAg                                                            >  2:1377031/1‑139 (MQ=255)

                                                                 gTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          <  1:279651/139‑1 (MQ=255)

                                                                 gTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          <  1:405538/139‑1 (MQ=255)

                                                                 gTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          <  1:1251382/139‑1 (MQ=255)

                                                                 gTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          <  1:152656/139‑1 (MQ=255)

                                                                   tCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCa                                                        <  2:194766/139‑1 (MQ=255)

                                                                     ccACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATc                                                      >  2:597856/1‑139 (MQ=255)

                                                                           tCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGa                                                <  2:1229373/139‑1 (MQ=255)

                                                                            ccTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGcc                                                                                 >  2:1597671/1‑105 (MQ=255)

                                                                            ccTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGcc                                                                                 <  1:1597671/105‑1 (MQ=255)

                                                                            ccTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGAc                                               <  1:180672/139‑1 (MQ=255)

                                                                            ccTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGAc                                               <  2:754297/139‑1 (MQ=255)

                                                                             cTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCAcc                                                                                                                      >  2:692424/1‑67 (MQ=255)

                                                                             cTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCAcc                                                                                                                      <  1:692424/67‑1 (MQ=255)

                                                                                           aCATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCg                                >  2:1008984/1‑139 (MQ=255)

                                                                                               cAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAg                            <  2:253483/139‑1 (MQ=255)

                                                                                                         gACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGc                       <  1:578964/134‑1 (MQ=255)

                                                                                                         gACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGc                       >  2:578964/1‑134 (MQ=255)

                                                                                                            gTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGAc                                               <  1:244219/107‑1 (MQ=255)

                                                                                                            gTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGAc                                               >  2:244219/1‑107 (MQ=255)

                                                                                                              cccACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGgcg             <  1:1786334/139‑1 (MQ=255)

                                                                                                               ccACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGgcgc            <  2:938024/139‑1 (MQ=255)

                                                                                                               ccACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGgcgc            >  1:392364/1‑139 (MQ=255)

                                                                                                                 acaGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAg          >  1:674363/1‑139 (MQ=255)

                                                                                                                     cTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGtt      >  1:882860/1‑139 (MQ=255)

                                                                                                                        tCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          >  1:904386/1‑84 (MQ=255)

                                                                                                                        tCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          <  2:904386/84‑1 (MQ=255)

                                                                                                                        tCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGc   >  2:1373247/1‑139 (MQ=255)

                                                                                                                        tCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGc   >  1:56784/1‑139 (MQ=255)

                                                                                                                         ccATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCa  >  1:352456/1‑139 (MQ=255)

                                                                                                                         ccATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCa  <  2:56784/139‑1 (MQ=255)

                                                                                                                          cATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          <  2:63123/82‑1 (MQ=255)

                                                                                                                          cATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          >  1:63123/1‑82 (MQ=255)

ACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCA  >  NC_000913/3161452‑3161711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A