Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,709,561	(C)_6→8	coding (915/1692 nt)	eptB ←	KDO phosphoethanolamine transferase, Ca(2+)‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,709,555	1	.	C	100.0%	287.9 / NA	80	G307G (GGC→GGG)	eptB	KDO phosphoethanolamine transferase, Ca(2+)‑inducible
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (35/45); total (35/45)
*	NC_000913	3,709,555	2	.	C	98.8%	283.6 / ‑3.7	80	coding (921/1692 nt)	eptB	KDO phosphoethanolamine transferase, Ca(2+)‑inducible
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (35/44); total (35/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

Only 100 of 114 total aligned reads displayed.

AGCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCCATA  >  NC_000913/3709417‑3709690

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aGCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgc‑‑g                                                                                                                                            <  1:1707916/139‑1 (MQ=255)

aGCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgc‑‑g                                                                                                                                            <  1:1439663/139‑1 (MQ=255)

 gCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgc‑‑g‑‑‑c                                                                                                                                        <  2:917419/139‑1 (MQ=255)

  cAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cc                                                                                                                                       <  2:524206/139‑1 (MQ=255)

    aTGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccc                                                                                                                                     >  1:222594/1‑139 (MQ=255)

     tgttgtCCTCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑ccccc                                                                                                                                    <  1:1091159/139‑1 (MQ=255)

      gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccc                                                                                                                                   >  2:1518209/1‑139 (MQ=255)

        tttCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGTTTATCTTCCGC‑‑G‑‑‑cccccccc                                                                                                                                 <  1:1055294/137‑3 (MQ=255)

        tgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccccc                                                                                                                                 <  1:84781/139‑3 (MQ=255)

        tgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccccc                                                                                                                                 <  1:1122164/139‑3 (MQ=255)

          tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccccctg                                                                                                                               >  2:537423/1‑135 (MQ=255)

          tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccccctg                                                                                                                               >  2:1033557/1‑135 (MQ=255)

             gCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccccctgacg                                                                                                                            <  1:645306/139‑8 (MQ=255)

              ccATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑ccccccc                                                                                                                                  >  1:587531/1‑131 (MQ=255)

              ccATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑ccccccc                                                                                                                                  <  2:587531/132‑2 (MQ=255)

               cATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑ccccccc                                                                                                                                  >  2:885301/1‑130 (MQ=255)

               cATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTCAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccccctgacgta                                                                                                                          <  1:918691/139‑10 (MQ=255)

                 tCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACa                                                                                                                          >  1:1189993/1‑139 (MQ=255)

                         cTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCa                                                                                                                  >  1:717959/1‑139 (MQ=255)

                          tGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCaa                                                                                                                 <  1:1681575/139‑1 (MQ=255)

                            tAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACg                                                                                                               <  2:127692/139‑1 (MQ=255)

                              gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAAcaca                                                                                                             >  1:1553176/1‑136 (MQ=255)

                              gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCa                                                                                                             >  1:618508/1‑139 (MQ=255)

                              gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCa                                                                                                             <  2:257594/139‑1 (MQ=255)

                                 ccACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTg                                                                                                          >  2:174054/1‑139 (MQ=255)

                                  cacaTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTga                                                                                                         <  1:1033557/139‑1 (MQ=255)

                                    caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTgaga                                                                                                       >  1:270912/1‑139 (MQ=255)

                                      tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGt                                                                                                     >  2:84590/1‑139 (MQ=255)

                                         cGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTg                                                                                                  >  2:529360/1‑139 (MQ=255)

                                          gCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GC‑‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                                <  2:305827/139‑1 (MQ=255)

                                           ctctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                                <  1:529360/139‑1 (MQ=255)

                                            tctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGtt                                                                                               <  1:84590/139‑1 (MQ=255)

                                                cATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           <  1:1348869/139‑1 (MQ=255)

                                                cATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           <  2:1390350/139‑1 (MQ=255)

                                                cATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           <  1:537423/139‑1 (MQ=255)

                                                   aGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           <  1:1317137/136‑1 (MQ=255)

                                                   aGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           >  2:1317137/1‑136 (MQ=255)

                                                     cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                                                                      >  1:471408/1‑139 (MQ=255)

                                                     cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                                                                      >  1:956892/1‑139 (MQ=255)

                                                     cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                                                                      <  1:871348/139‑1 (MQ=255)

                                                      gTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac                                                                                     <  1:734426/139‑1 (MQ=255)

                                                       tAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcaca                                                                                    <  1:293104/139‑1 (MQ=255)

                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                                >  1:610941/1‑124 (MQ=255)

                                                          agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGa                                                                                 >  2:443108/1‑139 (MQ=255)

                                                               aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTgg                                                                                                 <  2:1036671/118‑1 (MQ=255)

                                                               aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGACTAAc                                                                            >  2:41869/1‑139 (MQ=255)

                                                               aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccccctgacg                                                                                                                            <  1:608482/89‑8 (MQ=255)

                                                               aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccccctgacg                                                                                                                            >  2:608482/1‑82 (MQ=255)

                                                               aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCCTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTgg                                                                                                 >  1:1036671/1‑118 (MQ=255)

                                                                      aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGaa                                                                     >  2:1520226/1‑139 (MQ=255)

                                                                      aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGaa                                                                     <  2:270912/139‑1 (MQ=255)

                                                                           tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCaa                                                                                                                 <  2:299211/90‑1 (MQ=255)

                                                                           tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           >  2:446491/1‑112 (MQ=255)

                                                                           tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           <  1:446491/112‑1 (MQ=255)

                                                                           tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAcgccg                                                                >  2:1577825/1‑139 (MQ=255)

                                                                           tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccccctgacg                                                                                                                            <  1:1150368/77‑8 (MQ=255)

                                                                           tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccccctgacg                                                                                                                            >  2:1150368/1‑70 (MQ=255)

                                                                                  ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                                <  2:1430666/100‑1 (MQ=255)

                                                                                  ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                                >  1:1430666/1‑100 (MQ=255)

                                                                                  ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt                                                                               >  1:1494625/1‑117 (MQ=255)

                                                                                  ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt                                                                               <  2:1494625/117‑1 (MQ=255)

                                                                                         ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTcc                                                  <  2:717959/139‑1 (MQ=255)

                                                                                         ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTcc                                                  <  2:1189993/139‑1 (MQ=255)

                                                                                         ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTcc                                                  <  1:443108/139‑1 (MQ=255)

                                                                                           gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑ccccccc                                                                                                                                  <  2:1065954/55‑2 (MQ=255)

                                                                                           gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGAGGATTATCTTCCGC‑‑G‑‑‑ccccccc                                                                                                                                  >  1:1065954/1‑54 (MQ=255)

                                                                                            cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccccctga                                                                                                                              >  1:229140/1‑53 (MQ=255)

                                                                                            cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑cccccccctga                                                                                                                              <  2:229140/58‑6 (MQ=255)

                                                                                              aaaaTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGc                                             <  1:174054/139‑1 (MQ=255)

                                                                                                    ttctgttttTTTAATGTGCGCTGCGGATTATCTTCCGCCAC‑‑‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGttt                                       <  1:41869/139‑1 (MQ=255)

                                                                                                         ttctTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                                  <  2:471408/139‑1 (MQ=255)

                                                                                                          tctTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCCCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                                  <  2:222594/139‑1 (MQ=255)

                                                                                                          tctTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGt                                 <  2:494133/139‑1 (MQ=255)

                                                                                                           ctTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGaa                                                                     <  2:1601209/102‑1 (MQ=255)

                                                                                                           ctTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGaa                                                                     >  1:1601209/1‑102 (MQ=255)

                                                                                                           ctTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg                                <  2:618508/139‑1 (MQ=255)

                                                                                                           ctTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg                                <  1:1729472/139‑1 (MQ=255)

                                                                                                            ttttAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTgg                               >  2:364918/1‑139 (MQ=255)

                                                                                                              ttAATGTGCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGa                                                                                 <  1:121498/87‑1 (MQ=255)

                                                                                                                   gtgCGCTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACg                        >  1:1398846/1‑139 (MQ=255)

                                                                                                                      cgcTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           <  1:1289079/69‑1 (MQ=255)

                                                                                                                      cgcTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           <  2:535711/69‑1 (MQ=255)

                                                                                                                      cgcTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           >  1:535711/1‑69 (MQ=255)

                                                                                                                      cgcTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           >  2:1289079/1‑69 (MQ=255)

                                                                                                                      cgcTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                                  >  2:402985/1‑126 (MQ=255)

                                                                                                                      cgcTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                                  <  1:1674386/126‑1 (MQ=255)

                                                                                                                      cgcTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                                  >  2:1674386/1‑126 (MQ=255)

                                                                                                                      cgcTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                                  <  1:402985/126‑1 (MQ=255)

                                                                                                                      cgcTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg                                <  2:93275/128‑1 (MQ=255)

                                                                                                                      cgcTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg                                >  1:93275/1‑128 (MQ=255)

                                                                                                                      cgcTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg                                >  1:1385451/1‑128 (MQ=255)

                                                                                                                      cgcTGCGGATTATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg                                <  2:1385451/128‑1 (MQ=255)

                                                                                                                                tATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCaa                                                                                                                 <  1:363738/37‑1 (MQ=255)

                                                                                                                                tATCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCaa                                                                                                                 >  2:363738/1‑37 (MQ=255)

                                                                                                                                 aTCTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaaaa          <  1:1520226/139‑1 (MQ=255)

                                                                                                                                   cTTCCGC‑‑GCC‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           <  2:1419183/56‑1 (MQ=255)

                                                                                                                                      gcgc‑‑c‑‑‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGc                                             <  2:627794/96‑1 (MQ=255)

                                                                                                                                      gcgc‑‑c‑‑‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTGTCTCCTggg                                              <  1:838007/95‑1 (MQ=255)

                                                                                                                                       cgc‑‑c‑‑‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt                                                                               <  2:1296338/62‑1 (MQ=255)

                                                                                                                                       cgc‑‑c‑‑‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCCata  <  1:363676/139‑1 (MQ=255)

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AGCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGC‑‑G‑‑‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCCATA  >  NC_000913/3709417‑3709690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A