Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 757 76.1 3598070 93.4% 3360597 108.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,934,439 C→T A221V (GCT→GTT)  rbsA → D‑ribose ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,934,4390CT100.0% 174.4 / NA 55A221V (GCT→GTT) rbsAD‑ribose ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/29);  total (26/29)

CGAAACCGAATCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGCTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAATATCCGCACCTGGACAAAGCGCCGGGAGATATCCGCCTGAAAGTCGATAATCTCTG  >  NC_000913/3934302‑3934568
                                                                                                                                         |                                                                                                                                 
cgaaaccgaaTCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGAT‑GGCAATTTAttgttg                                                                                                                                 <  2:704035/139‑1 (MQ=255)
        aaTCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGa                                                                                                                          <  1:91674/139‑1 (MQ=255)
        aaTCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGa                                                                                                                          <  2:1475667/139‑1 (MQ=255)
               gTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCa                                                                                                                   <  1:1642970/139‑1 (MQ=255)
                   cgcgTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCAc                                                                                                               >  2:373421/1‑139 (MQ=255)
                     cgTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCa                                                                                                                <  2:92225/136‑1 (MQ=255)
                     cgTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCa                                                                                                                <  2:358896/136‑1 (MQ=255)
                     cgTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCa                                                                                                                >  1:92225/1‑136 (MQ=255)
                     cgTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCa                                                                                                                >  1:358896/1‑136 (MQ=255)
                     cgTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTg                                                                                                             >  1:844996/1‑139 (MQ=255)
                     cgTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTg                                                                                                             >  1:1031772/1‑139 (MQ=255)
                     cgTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTg                                                                                                             >  1:347528/1‑139 (MQ=255)
                       tCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGAc                                                                                                           <  1:1217838/139‑1 (MQ=255)
                                 gCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCg                                                                                                 <  2:655904/139‑1 (MQ=255)
                                 gCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCg                                                                                                 <  2:1147609/139‑1 (MQ=255)
                                  cTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGc                                                                                                <  1:1544975/139‑1 (MQ=255)
                                  cTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGc                                                                                                >  1:1672825/1‑139 (MQ=255)
                                       aTCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGAtt                                                                                           <  2:1017274/139‑1 (MQ=255)
                                             aGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAgatg                                                                                     <  2:1774999/139‑1 (MQ=255)
                                               gCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGAt                                                                                            >  1:67288/1‑130 (MQ=255)
                                               gCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGAt                                                                                            <  2:67288/130‑1 (MQ=255)
                                                   cgGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGAtggtg                                                                               >  2:1601797/1‑139 (MQ=255)
                                                   aggTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGAtggtg                                                                               >  1:1123536/2‑139 (MQ=255)
                                                          gTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCa                                                                        <  1:702434/139‑1 (MQ=255)
                                                                atCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGAtt                                                                                           <  1:941649/114‑1 (MQ=255)
                                                                atCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGAtt                                                                                           >  2:941649/1‑114 (MQ=255)
                                                                atCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTgaga                                                                                       >  1:1281083/1‑118 (MQ=255)
                                                                atCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTgaga                                                                                       <  2:1281083/118‑1 (MQ=255)
                                                                atCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTgg                                                                  >  1:935113/1‑139 (MQ=255)
                                                                atCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTgg                                                                  <  2:347528/139‑1 (MQ=255)
                                                                  ctcCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGaa                                                                <  2:844996/139‑1 (MQ=255)
                                                                  ctcCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGaa                                                                >  1:210540/1‑139 (MQ=255)
                                                                     ccACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCg                                                                          <  2:1435910/126‑1 (MQ=255)
                                                                     ccACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCg                                                                          >  1:1435910/1‑126 (MQ=255)
                                                                     ccACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGAt                                                             >  1:188458/1‑139 (MQ=255)
                                                                           cATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAatat                                                       >  1:503651/1‑139 (MQ=255)
                                                                           cATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAatat                                                       >  2:535182/1‑139 (MQ=255)
                                                                            aTGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGGTCAATATc                                                      <  2:210540/139‑1 (MQ=255)
                                                                            aTGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAATATc                                                      <  2:935113/139‑1 (MQ=255)
                                                                                          cGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAATATCCGCACCTGGACaaa                                        >  1:514870/1‑139 (MQ=255)
                                                                                              aTTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAATATCCGCACCTGGACAAAgcgc                                    <  1:1601797/139‑1 (MQ=255)
                                                                                                       gACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTgaga                                                                                       <  2:247120/79‑1 (MQ=255)
                                                                                                       gACGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTgaga                                                                                       >  1:247120/1‑79 (MQ=255)
                                                                                                        aCGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGAtt                                                                                           >  1:434782/1‑74 (MQ=255)
                                                                                                        aCGTTACCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGAtt                                                                                           <  2:434782/74‑1 (MQ=255)
                                                                                                             aCCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAATATCCGCACCTGGACAAAGCGcc                                   <  1:160155/125‑1 (MQ=255)
                                                                                                             aCCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAATATCCGCACCTGGACAAAGCGcc                                   >  2:160155/1‑125 (MQ=255)
                                                                                                             aCCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAATATCCGCACCTGGACAAAGCGCCGGGAGATATCCGcc                     >  2:1584674/1‑139 (MQ=255)
                                                                                                             aCCGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAATATCCGCACCTGGACAAAGCGCCGGGAGATATCCGcc                     <  1:535182/139‑1 (MQ=255)
                                                                                                               cGTTTTTCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAATATCCGCACCTGGACAAAGCGCCGGGAGATATCCGCCTg                   <  2:1672825/139‑1 (MQ=255)
                                                                                                                  ttttCGTGATGGGCAATTTATTGTTGAGCGCGAAGTg                                                                                                                      <  2:1653543/37‑1 (MQ=38)
                                                                                                                  ttttCGTGATGGGCAATTTATTGTTGAGCGCGAAGTg                                                                                                                      >  1:1653543/1‑37 (MQ=255)
                                                                                                                   tttCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGAtggt                                                                                >  1:1631441/1‑74 (MQ=255)
                                                                                                                   tttCGTGATGGGCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGAtggt                                                                                <  2:1631441/74‑1 (MQ=255)
                                                                                                                              gCAATTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAATATCCGCACCTGGACAAAGCGCCGGGAGATATCCGCCTGAAAGTCGATAAtctc    >  1:1480424/1‑139 (MQ=255)
                                                                                                                                aaTTTATTGTTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAATATCCGCACCTGGACAAAGCGCCGGGAGATATCCGCCTGAAAGTCGATAATCTCTg  <  2:503651/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                 
CGAAACCGAATCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATGACGTTACCGTTTTTCGTGATGGGCAATTTATTGCTGAGCGCGAAGTGGCATCACTGACCGAAGATTCGCTGATTGAGATGATGGTGGGTCGCAAGCTGGAAGATCAATATCCGCACCTGGACAAAGCGCCGGGAGATATCCGCCTGAAAGTCGATAATCTCTG  >  NC_000913/3934302‑3934568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A