Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
764 |
42.0 |
2018750 |
95.5% |
1927906 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,503,952 |
G→A |
*262* (TAG→TAA) |
frlD → |
fructoselysine 6‑kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,503,952 | 0 | G | A | 100.0%
| 90.9
/ NA
| 29 | *262* (TAG→TAA) | frlD | fructoselysine 6‑kinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/15); total (14/15) |
CGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACT > NC_000913/3503817‑3504073
|
cGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑ggtaagta < 1:257131/139‑1 (MQ=255)
cATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGt > 1:790844/1‑139 (MQ=255)
tGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTg > 1:538456/1‑139 (MQ=255)
gTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCg < 2:538456/139‑1 (MQ=255)
cGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAg < 2:790844/139‑1 (MQ=255)
aTTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCa < 1:246491/139‑1 (MQ=255)
tGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAAc < 1:922614/139‑1 (MQ=255)
cTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTAc > 1:581863/1‑139 (MQ=255)
gCGGGGATGACATTACCGCAGGTGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCt > 1:985127/1‑104 (MQ=255)
gCGGGGATGACATTACCGCAGGTGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCt < 2:985127/104‑1 (MQ=255)
cGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa < 1:912259/134‑1 (MQ=255)
cGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa > 2:912259/1‑134 (MQ=255)
aTGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCtata > 2:517103/1‑139 (MQ=255)
tGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATaa > 2:172540/1‑139 (MQ=255)
gACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAAt < 1:172540/139‑1 (MQ=255)
aCATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCGACGTGCTGTTACTTGCCGGAAAACg < 2:906713/128‑1 (MQ=255)
aCATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCGACGTGCTGTTACTTGCCGGAAAACg > 1:906713/1‑128 (MQ=255)
tACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATaa < 1:950096/133‑1 (MQ=255)
tACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATaa > 2:950096/1‑133 (MQ=255)
cGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa > 2:230852/1‑135 (MQ=255)
cGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa < 1:230852/135‑1 (MQ=255)
aGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTa > 1:919041/1‑129 (MQ=255)
aGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTa < 2:919041/129‑1 (MQ=255)
aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTc > 2:696472/1‑139 (MQ=255)
tGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTa < 2:401984/139‑1 (MQ=255)
tACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca < 2:744268/96‑1 (MQ=255)
tACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca > 1:744268/1‑96 (MQ=255)
tACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTAct < 1:337197/139‑1 (MQ=255)
tGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg < 2:14931/121‑1 (MQ=255)
tGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg > 1:14931/1‑121 (MQ=255)
|
CGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACT > NC_000913/3503817‑3504073
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
