Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,506,024	T→C	intergenic (‑4/+8)	yhfS ← / ← yhfT	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein/inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,506,024	0	T	C	100.0%	110.5 / NA	32	intergenic (‑4/+8)	yhfS/yhfT	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein/inner membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/19); total (13/19)

AATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGATTCGTTGAGGAAGTAAA  >  NC_000913/3505903‑3506156

aaTCACCGCCAGTAAGAAGCTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAg                                                                                                                          >  1:559867/1‑134 (MQ=255)

aaTCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAg                                                                                                                          <  2:559867/134‑1 (MQ=255)

aaTCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGga                                                                                                                     <  2:887107/139‑1 (MQ=255)

 aTCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGgaa                                                                                                                    >  2:853810/1‑139 (MQ=255)

          aGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGc                                                                                                           <  1:25686/139‑1 (MQ=255)

              aGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCACGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAg                                                                                                       >  1:612258/1‑139 (MQ=255)

                   cTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGt                                                                                                  <  1:246763/139‑1 (MQ=255)

                       cTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAg                                                                                                       <  1:1007215/130‑1 (MQ=255)

                       cTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAg                                                                                                       >  2:1007215/1‑130 (MQ=255)

                        tGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGa                                                                                             <  1:146517/139‑1 (MQ=255)

                                         cAAATGCTATCCACCAGCTCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATAcc                                                                            <  2:632724/139‑1 (MQ=255)

                                          aaaTGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCg                                                                           <  2:612258/139‑1 (MQ=255)

                                                tATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATc                                                                     >  2:767514/1‑139 (MQ=255)

                                                      ccaGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACt                                                               >  1:528132/1‑139 (MQ=255)

                                                       caGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGgaacgaa                                                                                                                <  1:4852/89‑1 (MQ=255)

                                                       caGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGgaacgaa                                                                                                                >  2:4852/1‑89 (MQ=255)

                                                          cgcAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGcc                                                                                                                                   <  1:540604/67‑1 (MQ=255)

                                                          cgcAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGcc                                                                                                                                   >  2:540604/1‑67 (MQ=255)

                                                            cAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACg                                                         >  2:870423/1‑139 (MQ=255)

                                                                    ttttGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTgc                                                 <  2:528132/139‑1 (MQ=255)

                                                                      ttGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACAATCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTgccg                                               <  2:319209/139‑1 (MQ=255)

                                                                                      atCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCgg                               >  2:914489/1‑139 (MQ=255)

                                                                                       tCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                              <  1:767514/139‑1 (MQ=255)

                                                                                       tCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGCGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                              <  1:853810/139‑1 (MQ=255)

                                                                                            aGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGcc                         <  2:558973/139‑1 (MQ=255)

                                                                                            aGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGcc                         <  1:565531/139‑1 (MQ=255)

                                                                                                        ggAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTcag                                                                                     >  2:112548/1‑67 (MQ=255)

                                                                                                        ggAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTcag                                                                                     <  1:112548/67‑1 (MQ=255)

                                                                                                         gAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGATTCGTTg            <  1:528883/139‑1 (MQ=255)

                                                                                                                 ttCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                              <  2:391229/113‑1 (MQ=255)

                                                                                                                 ttCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                              >  1:391229/1‑113 (MQ=255)

                                                                                                                   cATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGATTCGTTGAGGAAGTaaa  >  1:956700/1‑139 (MQ=255)

AATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGATTCGTTGAGGAAGTAAA  >  NC_000913/3505903‑3506156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A