Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,920,950	C→T	80 (TAG→TAA)	atpE ←	F0 sector of membrane‑bound ATP synthase, subunit c

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,920,950	0	C	T	100.0%	154.8 / NA	48	80 (TAG→TAA)	atpE	F0 sector of membrane‑bound ATP synthase, subunit c
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/24); total (24/24)

CGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCGCCTTCCA  >  NC_000913/3920816‑3921083

cGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTA‑TTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcgc                                                                                                                                  >  1:773906/1‑139 (MQ=255)

        aTGCAGAACAGAACGAACAGGACAAACGCGATGACCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCg                                                                                                                           <  2:383003/139‑1 (MQ=255)

         tGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGa                                                                                                                          >  2:538695/1‑139 (MQ=255)

         tGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGa                                                                                                                          >  1:292179/1‑139 (MQ=255)

         tGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGa                                                                                                                          <  2:785765/139‑1 (MQ=255)

         tGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGa                                                                                                                          >  2:817215/1‑139 (MQ=255)

                  gaacgaacAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTA‑TTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTa                                                                                                                <  2:773906/139‑1 (MQ=255)

                   aacgaacAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTa                                                                                                                <  2:738481/139‑1 (MQ=255)

                     cgaacAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAca                                                                                                              <  1:538695/139‑1 (MQ=255)

                           ggACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagaccca                                                                                                        >  2:662382/1‑139 (MQ=255)

                                 aCGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAg                                                                                                                             <  2:911150/112‑1 (MQ=255)

                                 aCGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAg                                                                                                                             >  1:911150/1‑112 (MQ=255)

                                    cgATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAg                                                                                               >  1:186637/1‑139 (MQ=255)

                                               ccGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCggg                                                                                    >  2:397013/1‑139 (MQ=255)

                                                    gATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcaga                                                                                                            <  2:948685/110‑1 (MQ=255)

                                                    gATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcaga                                                                                                            >  1:948685/1‑110 (MQ=255)

                                                     aTTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCa                                                                              <  2:890381/139‑1 (MQ=255)

                                                         ttgCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATcca                                                                          >  2:896880/1‑139 (MQ=255)

                                                          tgCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccac                                                                         <  1:397013/139‑1 (MQ=255)

                                                              ttAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATGCACCAGa                                                                     <  1:662382/139‑1 (MQ=255)

                                                                   aTTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcg                                                                                                                                   <  1:576431/72‑1 (MQ=255)

                                                                   aTTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcg                                                                                                                                   >  2:576431/1‑72 (MQ=255)

                                                                   aTTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCAt                                                                <  2:66659/139‑1 (MQ=255)

                                                                     tCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGAATCCACCAGACCCATaa                                                              <  1:161786/139‑1 (MQ=255)

                                                                              aaTGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCg                                                                                             <  1:339462/99‑1 (MQ=255)

                                                                              aaTGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCg                                                                                             >  2:339462/1‑99 (MQ=255)

                                                                                tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTAc                                                                                                 <  2:768567/93‑1 (MQ=255)

                                                                                tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTAc                                                                                                 >  1:768567/1‑93 (MQ=255)

                                                                                tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGa                                                                                   <  1:497517/107‑1 (MQ=255)

                                                                                tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGa                                                                                   >  2:497517/1‑107 (MQ=255)

                                                                                tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAAc                                                   >  2:186411/1‑139 (MQ=255)

                                                                                 gCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACt                                                  <  2:186637/139‑1 (MQ=255)

                                                                                 gCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACt                                                  <  2:292179/139‑1 (MQ=255)

                                                                                   ctctATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGa                                                >  2:570739/1‑139 (MQ=255)

                                                                                        tttAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAAc                                                                                                                        >  2:196129/1‑62 (MQ=255)

                                                                                        tttAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAAc                                                                                                                        <  1:196129/62‑1 (MQ=255)

                                                                                             ttAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACgcagca                                      <  1:896880/139‑1 (MQ=255)

                                                                                                aCGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCaga                                    >  2:372165/1‑138 (MQ=255)

                                                                                                aCGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCaga                                    <  1:372165/138‑1 (MQ=255)

                                                                                                  gTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCa                                                                                                                    >  1:95903/1‑56 (MQ=255)

                                                                                                  gTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCa                                                                                                                    <  2:95903/56‑1 (MQ=255)

                                                                                                              ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGt                     >  2:161296/1‑139 (MQ=255)

                                                                                                              ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGt                     >  1:340711/1‑139 (MQ=255)

                                                                                                                  ttAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAca                                                                                                              >  2:405906/1‑46 (MQ=255)

                                                                                                                  ttAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAca                                                                                                              <  1:405906/46‑1 (MQ=255)

                                                                                                                   taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGAcg                >  1:766896/1‑139 (MQ=255)

                                                                                                                          aaGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTgcg         <  1:798580/139‑1 (MQ=255)

                                                                                                                                 gCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCGCCTTCCa  >  1:722072/1‑139 (MQ=255)

CGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCGCCTTCCA  >  NC_000913/3920816‑3921083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A