Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I2 R1 760 52.7 2744488 90.2% 2475528 105.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,544,074 C→T *257* (TAG→TAA)  bioH ← pimeloyl‑ACP methyl ester carboxylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,544,0740CT100.0% 201.0 / NA 63*257* (TAG→TAA) bioHpimeloyl‑ACP methyl ester carboxylesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/32);  total (31/32)

AGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGC  >  NC_000913/3543952‑3544194
                                                                                                                          |                                                                                                                        
aGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCa                                                                                                          >  1:483208/1‑139 (MQ=255)
aGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCa                                                                                                          <  2:249048/139‑1 (MQ=255)
 gCCAAAATAATGCTTGAATGCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTCGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCaa                                                                                                         >  2:913048/1‑139 (MQ=255)
  ccAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAAc                                                                                                        <  2:411368/139‑1 (MQ=255)
     aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGGGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGCCGAGTCAGAAAAGTTCTGTCGAGCAATTTCAAAAGACACTTACACCCTCAGCTTCAACGcc                                                                                                     >  1:411368/1‑139 (MQ=255)
     aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCaa                                                                                                         <  2:403934/135‑1 (MQ=255)
     aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCaa                                                                                                         >  1:403934/1‑135 (MQ=255)
     aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGcc                                                                                                     >  1:1205703/1‑139 (MQ=255)
     aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGcc                                                                                                     >  1:430591/1‑139 (MQ=255)
            gCTTGAATCCGAAGTTCCCCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCCGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACcagca                                                                                              >  1:794558/1‑139 (MQ=255)
                gAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTg                                                                                          >  1:822925/1‑139 (MQ=255)
                  aTCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGAc                                                                                        <  1:323319/139‑1 (MQ=255)
                     cGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACaaa                                                                                     >  2:504689/1‑139 (MQ=255)
                       gAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAAc                                                                                   >  1:706632/1‑139 (MQ=255)
                            ccTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGccacc                                                                                                  <  1:631737/119‑1 (MQ=255)
                            ccTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGccacc                                                                                                  >  2:631737/1‑119 (MQ=255)
                                 tGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGAt                                                                         <  1:1349011/139‑1 (MQ=255)
                                        atcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaat                                                                  >  1:1315431/1‑139 (MQ=255)
                                        atcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCGCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaat                                                                  >  2:481759/1‑139 (MQ=255)
                                          catGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaataa                                                                >  1:213656/1‑139 (MQ=255)
                                          catGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaataa                                                                <  2:794558/139‑1 (MQ=255)
                                            tGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaataaat                                                              >  1:1046376/1‑139 (MQ=255)
                                             gcgcTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATg                                                             <  1:188173/139‑1 (MQ=255)
                                                    aCCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATg                                                      <  2:430591/139‑1 (MQ=255)
                                                        ggCTGTCAGAAGAAGAGTTGCCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGcc                                                  >  1:805956/1‑139 (MQ=255)
                                                             tCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTaca                                                                                                                      <  2:748397/66‑1 (MQ=255)
                                                             tCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTaca                                                                                                                      >  1:748397/1‑66 (MQ=255)
                                                               agaagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTgg                                           <  2:1205703/139‑1 (MQ=255)
                                                               agaagaagaGTTGGCTGCGGCCAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTgg                                           <  2:805956/139‑1 (MQ=255)
                                                                 aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                         <  2:968722/139‑1 (MQ=255)
                                                                 aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                         <  2:483208/139‑1 (MQ=255)
                                                                 aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                         <  2:822925/139‑1 (MQ=255)
                                                                  agaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGa                                        <  2:220109/139‑1 (MQ=255)
                                                                       agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACAccc                                                                                                                   >  2:408611/1‑59 (MQ=255)
                                                                       agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACAccc                                                                                                                   <  1:408611/59‑1 (MQ=255)
                                                                       agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCct                                                                                                                  >  2:859793/1‑60 (MQ=255)
                                                                       agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCct                                                                                                                  <  1:859793/60‑1 (MQ=255)
                                                                       agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGata                                   >  1:848802/1‑139 (MQ=255)
                                                                        gTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTGACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCTCTTTGGCGAAGatat                                  <  1:913048/139‑1 (MQ=255)
                                                                         ttGGCTGCGGCGAGTCAGTAAAGTTCTGTATCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGatata                                 >  2:912204/1‑139 (MQ=255)
                                                                           ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCaa                                                                                                         >  2:591747/1‑65 (MQ=255)
                                                                           ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCaa                                                                                                         <  1:591747/65‑1 (MQ=255)
                                                                           ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATg                               >  2:18425/1‑139 (MQ=255)
                                                                           ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATg                               >  1:782930/1‑139 (MQ=255)
                                                                           ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATg                               >  2:225253/1‑139 (MQ=255)
                                                                                 ggcgAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGc                         >  2:266936/1‑139 (MQ=255)
                                                                                   cgAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCtg                       <  1:504689/139‑1 (MQ=255)
                                                                                    gAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCtgt                      <  2:1315431/139‑1 (MQ=255)
                                                                                    gAGTCAGTAAAATTCTGTCGCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGTCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGGATGGGCCGCTTTGGCGAAGATATATGATTCGCtgt                      <  1:1319377/139‑1 (MQ=255)
                                                                                      gTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGa                                        >  1:1225346/1‑119 (MQ=255)
                                                                                      gTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGa                                        <  2:1225346/119‑1 (MQ=255)
                                                                                          gTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGcc                <  2:706632/139‑1 (MQ=255)
                                                                                          gTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGcc                <  2:848802/139‑1 (MQ=255)
                                                                                           tAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCg                                                 >  1:110033/1‑105 (MQ=255)
                                                                                           tAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCg                                                 <  2:110033/105‑1 (MQ=255)
                                                                                                 tCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGttt         >  1:684368/1‑139 (MQ=255)
                                                                                                 tCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGATGTGAGGCCAAAGttt         >  1:618733/1‑139 (MQ=255)
                                                                                                    gTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATc      <  1:225253/139‑1 (MQ=255)
                                                                                                    gTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATc      <  1:18425/139‑1 (MQ=255)
                                                                                                     tctcGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCGTGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATcc     <  2:782930/139‑1 (MQ=255)
                                                                                                          ccATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGc                                                                              <  1:99656/61‑1 (MQ=255)
                                                                                                          ccATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGc                                                                              >  2:99656/1‑61 (MQ=255)
                                                                                                                       cACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGc  <  1:1116698/124‑1 (MQ=255)
                                                                                                                          |                                                                                                                        
AGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGC  >  NC_000913/3543952‑3544194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A