Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,914,462	A→G	L180P (CTG→CCG)	rlmD ←	23S rRNA m(5)U1939 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,914,462	0	A	G	100.0%	70.8 / NA	21	L180P (CTG→CCG)	rlmD	23S rRNA m(5)U1939 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/10); total (11/10)

CCAGTTTTTCGCGATCTGCCGAACTTAGCGGTGCGGTATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCAGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCA  >  NC_000913/2914343‑2914597

ccAGTTTTTCGCGATCTGCCGAACTTAGCGGTGCGGTATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTggg                                                                                                                      <  1:36342/139‑1 (MQ=255)

         cgcgATCTGCCGAACTTAGCGGTGCGGTATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGc                                                                                                             >  1:704389/1‑139 (MQ=255)

                  ccGAACTTAGCGGTGCGGTATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGAc                                                                                                                                      >  1:447168/1‑105 (MQ=255)

                      aCTTAGCGGTGCGGTATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGgc                                                                                                <  2:538639/139‑1 (MQ=255)

                                 cggtATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATggg                                                                                     >  1:5307/1‑139 (MQ=255)

                                  ggtATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATgggg                                                                                    <  2:48275/139‑1 (MQ=255)

                                           cAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTg                                                                           >  2:731968/1‑139 (MQ=255)

                                             aaaTCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGAc                                                                         <  1:281498/139‑1 (MQ=255)

                                                  atcaGCGTGCCGCTGGTTGTCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGa                                                                    <  1:625192/139‑1 (MQ=255)

                                                            cGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCa                                                                                  <  2:543062/115‑1 (MQ=255)

                                                            cGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCa                                                                                  >  1:543062/1‑115 (MQ=255)

                                                                         tACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGgcagc                                                                                                                  <  1:618382/70‑1 (MQ=255)

                                                                         tACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGgcagc                                                                                                                  >  2:618382/1‑70 (MQ=255)

                                                                               ttCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCGCTTTGCGAAAc                                       <  2:563068/139‑1 (MQ=255)

                                                                               ttCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCGCTTTGCGAAAc                                       >  1:206980/1‑139 (MQ=255)

                                                                                                       aGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTc               >  2:129467/1‑139 (MQ=255)

                                                                                                       aGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCACTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTc               >  1:466334/1‑139 (MQ=255)

                                                                                                                   gCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACaa                                                                 <  1:247695/77‑1 (MQ=255)

                                                                                                                   gCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACaa                                                                 >  2:247695/1‑77 (MQ=255)

                                                                                                                   gCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACGATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTc   <  2:747355/139‑1 (MQ=255)

                                                                                                                    cccGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCa  >  1:769391/1‑139 (MQ=255)

CCAGTTTTTCGCGATCTGCCGAACTTAGCGGTGCGGTATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCAGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCA  >  NC_000913/2914343‑2914597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A