Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,075,233	A→G	I317T (ATA→ACA)	yggF ←	fructose 1,6 bisphosphatase isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,075,233	0	A	G	100.0%	143.3 / NA	40	I317T (ATA→ACA)	yggF	fructose 1,6 bisphosphatase isozyme
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/19); total (21/19)

CCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTC  >  NC_000913/3075097‑3075367

ccccTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtgtt                                                                                                                                      <  2:28154/139‑1 (MQ=255)

ccccTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtgtt                                                                                                                                      <  2:344815/139‑1 (MQ=255)

                gTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGt                                                                                                                      <  2:374487/139‑1 (MQ=255)

                   tGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACGCGTTTGGTCcg                                                                                                                   >  1:504654/1‑139 (MQ=255)

                   tGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCcg                                                                                                                   <  1:390278/139‑1 (MQ=255)

                     cgcAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCcgcg                                                                                                                 <  2:144275/139‑1 (MQ=255)

                        aGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGcgccg                                                                                                              >  1:197528/1‑139 (MQ=255)

                        aGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTACCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGcgccg                                                                                                              <  2:425841/139‑1 (MQ=255)

                          ttttCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCgccgcc                                                                                                            <  1:86693/139‑1 (MQ=255)

                          ttttCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCgccgcc                                                                                                            <  1:294048/139‑1 (MQ=255)

                                       aaataaatTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTc                                                                                               <  2:248318/139‑1 (MQ=255)

                                                  ccTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCAc                                                                                    >  2:10184/1‑139 (MQ=255)

                                                     gtgCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCAcccc                                                                                 >  1:520043/1‑139 (MQ=255)

                                                          tCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCg                                                                            <  1:10184/139‑1 (MQ=255)

                                                           ccAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGa                                                                                                          <  1:266370/108‑1 (MQ=255)

                                                           ccAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGa                                                                                                          >  2:266370/1‑108 (MQ=255)

                                                                  cTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTa                                                                                                    >  2:240316/1‑107 (MQ=255)

                                                                  cTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTa                                                                                                    <  1:240316/107‑1 (MQ=255)

                                                                  cTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGt                                                                    >  2:512129/1‑139 (MQ=255)

                                                                  cTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGt                                                                    >  2:539994/1‑139 (MQ=255)

                                                                            tcatcTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGt                                                          >  2:504516/1‑139 (MQ=255)

                                                                                    tGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGc                                                  >  2:622211/1‑139 (MQ=255)

                                                                                     gTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCt                                                 >  2:728934/1‑139 (MQ=255)

                                                                                       cAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCa                                               >  1:245297/1‑139 (MQ=255)

                                                                                          cGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCg                                            <  2:245297/139‑1 (MQ=255)

                                                                                               ccATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGt                                       <  1:154993/139‑1 (MQ=255)

                                                                                                         tCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTa                             >  1:720240/1‑139 (MQ=255)

                                                                                                         tCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTa                             >  1:343887/1‑139 (MQ=255)

                                                                                                             aTAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAgcgc                         >  1:86598/1‑139 (MQ=255)

                                                                                                                gATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTaa                      <  2:720240/139‑1 (MQ=255)

                                                                                                                gATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTaa                      <  1:60428/139‑1 (MQ=255)

                                                                                                                           aTGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAg                                                                                                      >  2:454029/1‑48 (MQ=255)

                                                                                                                           aTGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAg                                                                                                      <  1:454029/48‑1 (MQ=255)

                                                                                                                           aTGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCa                                                                                     <  2:728891/65‑1 (MQ=255)

                                                                                                                           aTGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCa                                                                                     >  1:728891/1‑65 (MQ=255)

                                                                                                                            tGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGt          <  2:655992/139‑1 (MQ=255)

                                                                                                                            tGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGt          >  1:655992/1‑139 (MQ=255)

                                                                                                                               aGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTcc       <  1:539994/139‑1 (MQ=255)

                                                                                                                                 ggAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAaga                   <  1:52198/125‑1 (MQ=255)

                                                                                                                                 ggAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAaga                   >  2:52198/1‑125 (MQ=255)

                                                                                                                                   aGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCt   >  1:424804/1‑139 (MQ=255)

                                                                                                                                    gTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTc  >  1:297638/1‑139 (MQ=255)

CCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTC  >  NC_000913/3075097‑3075367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A