Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,968,037	A→G	K41R (AAA→AGA)	wecA →	UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,968,037	0	A	G	100.0%	164.7 / NA	48	K41R (AAA→AGA)	wecA	UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/26); total (22/26)

TGAGAGCATGCATTGTGAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAAACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGT  >  NC_000913/3967902‑3968166

actgtcagtaaatTGTGAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACg                                                                                                                                >  1:625953/12‑139 (MQ=255)

        tgcatTGTGAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCAc                                                                                                                            >  2:507601/1‑135 (MQ=255)

        tgcatTGTGAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCAc                                                                                                                            <  1:507601/135‑1 (MQ=255)

              gtgAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGa                                                                                                                  >  2:698438/1‑139 (MQ=255)

              gtgAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGa                                                                                                                  <  1:333551/139‑1 (MQ=255)

              gtgAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGa                                                                                                                  >  2:400999/1‑139 (MQ=255)

               tgAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGAt                                                                                                                 >  2:107994/1‑139 (MQ=255)

                              tGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACg                                                                                                                                <  1:221274/109‑1 (MQ=255)

                              tGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACg                                                                                                                                >  2:221274/1‑109 (MQ=255)

                                aGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGa                                                                                                >  1:280886/1‑139 (MQ=255)

                                 gTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCa                                                                                                                          >  2:38927/1‑112 (MQ=255)

                                 gTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCa                                                                                                                          <  1:38927/112‑1 (MQ=255)

                                      gATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGAc                                                                                                                                 <  2:571934/100‑1 (MQ=255)

                                      gATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGAc                                                                                                                                 >  1:571934/1‑100 (MQ=255)

                                      gATCTCATCAGTATTATTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCgg                                                                                          >  1:73982/1‑139 (MQ=255)

                                       aTCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACg                                                                                                                                <  1:97908/100‑1 (MQ=255)

                                       aTCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACg                                                                                                                                >  2:97908/1‑100 (MQ=255)

                                        tctcATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGtt                                                                                        >  2:497163/1‑139 (MQ=255)

                                            atcaGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACg                                                                                    <  1:437273/139‑1 (MQ=255)

                                            atcaGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACg                                                                                    >  2:64226/1‑139 (MQ=255)

                                            atcaGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACg                                                                                    >  2:437273/1‑139 (MQ=255)

                                              caGTATTATTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCa                                                                                  <  2:73982/139‑1 (MQ=255)

                                                  aTTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGa                                                                              <  2:186485/139‑1 (MQ=255)

                                                   tttttttATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATAc                                                                                                               <  1:606884/105‑1 (MQ=255)

                                                   tttttttATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATAc                                                                                                               >  2:606884/1‑105 (MQ=255)

                                                      ttttATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTg                                                                          >  2:324429/1‑139 (MQ=255)

                                                         tATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCtt                                                                       <  1:132740/139‑1 (MQ=255)

                                                                    ctgtttctgtttTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGaa                                                            <  1:698438/139‑1 (MQ=255)

                                                                     tgtttctgtttTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAAt                                                           >  2:409187/1‑139 (MQ=255)

                                                                       tttctgtttTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTg                                                         >  1:525780/1‑139 (MQ=255)

                                                                       tttctgtttTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTg                                                         >  2:725499/1‑139 (MQ=255)

                                                                       tttctgtttTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTg                                                         <  1:400999/139‑1 (MQ=255)

                                                                         tctgtttTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTc                                                       <  1:409187/139‑1 (MQ=255)

                                                                           tgtttTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGa                                                     <  1:366389/139‑1 (MQ=255)

                                                                           tgtttTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGa                                                     <  2:625953/139‑1 (MQ=255)

                                                                                tttGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACt                                                <  2:759630/139‑1 (MQ=255)

                                                                                 ttGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGAtt                                                                                              >  1:372029/1‑92 (MQ=255)

                                                                                 ttGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGAtt                                                                                              <  2:372029/92‑1 (MQ=255)

                                                                                  tGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACtat                                              <  1:324429/139‑1 (MQ=255)

                                                                                         aaGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCgc                                       <  2:2285/139‑1 (MQ=255)

                                                                                          aGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCgca                                      <  1:240330/139‑1 (MQ=255)

                                                                                             tGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCgcatgc                                   <  2:406363/139‑1 (MQ=255)

                                                                                              ggCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCgcatgca                                  <  2:174489/139‑1 (MQ=255)

                                                                                              ggCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGCGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCgcatgca                                  <  2:525780/139‑1 (MQ=255)

                                                                                                  aaaaaaGTCGGTTTAGTGGATAAACCAAACTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCAtctc                              <  1:64226/139‑1 (MQ=255)

                                                                                                                          ccAAACTTCCAAAGACGTCACCAGGGATTGATACCTCTCGGTGGAGGGATTTCCGTTTACGCAGGTATTTGCTTCACCGTCGGAATTGTCGATTACTATATTCCGCTTGCATCTCTCTGCCTCGCTTGTCCCGGCGTGc      >  2:561046/1‑139 (MQ=255)

                                                                                                                              aCTTCCGCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGt  >  1:375649/1‑139 (MQ=255)

                                                                                                                                    gCAGACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCgc                                       <  1:444950/96‑1 (MQ=255)

TGAGAGCATGCATTGTGAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCCGTAAGGTGGCAAAAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAAACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGT  >  NC_000913/3967902‑3968166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A