Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I2 R1 757 38.1 1607544 96.5% 1551279 117.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,257,675 G→A G112G (GGC→GGT)  yhaO ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,257,6750GA100.0% 186.2 / NA 59G112G (GGC→GGT) yhaOputative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/30);  total (29/30)

ACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAA  >  NC_000913/3257546‑3257806
                                                                                                                                 |                                                                                                                                   
aCTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGttt                                                                                                                            <  1:416610/139‑1 (MQ=255)
              aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAAcc                                                                                                              >  1:751646/1‑139 (MQ=255)
              aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAAcc                                                                                                              >  1:227722/1‑139 (MQ=255)
              aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACACCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAAcc                                                                                                              >  2:775571/1‑139 (MQ=255)
                                     tGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCtt                                                                                       >  2:442896/1‑139 (MQ=255)
                                          gTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACAt                                                                                  >  2:717847/1‑139 (MQ=255)
                                             ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAAt                                                                                                                  >  1:33729/1‑104 (MQ=255)
                                             ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAAt                                                                                                                  <  2:33729/104‑1 (MQ=255)
                                             ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTaa                                                                                           >  1:275278/1‑127 (MQ=255)
                                             ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTaa                                                                                           <  2:275278/127‑1 (MQ=255)
                                                    cTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAACTAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGAtt                                                                        >  2:614492/1‑139 (MQ=255)
                                                    cTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGAtt                                                                        >  2:277252/1‑139 (MQ=255)
                                                        cGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGc                                                                    <  1:775571/139‑1 (MQ=255)
                                                         gTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGcc                                                                   <  1:614492/139‑1 (MQ=255)
                                                           tGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAg                                                                 <  1:581538/139‑1 (MQ=255)
                                                            ggTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGc                                                                >  1:647268/1‑139 (MQ=255)
                                                             gTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                             >  2:773811/1‑109 (MQ=255)
                                                             gTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                             <  1:773811/109‑1 (MQ=255)
                                                             gTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                               <  2:5113/139‑1 (MQ=255)
                                                             gTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                               <  1:277252/139‑1 (MQ=255)
                                                                 tGGCGGTGGAATAGACGAACATCCACATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTCTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                               <  1:519153/135‑1 (MQ=255)
                                                                 tGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAAt                                                                                                                  <  2:144051/84‑1 (MQ=255)
                                                                 tGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAAt                                                                                                                  >  1:144051/1‑84 (MQ=255)
                                                                 tGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACg                                                                                                    >  2:390637/1‑98 (MQ=255)
                                                                 tGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACg                                                                                                    <  1:390637/98‑1 (MQ=255)
                                                                 tGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTAtt                                                           >  1:447138/1‑139 (MQ=255)
                                                                 tGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTAAATTCTGGTGATTCTGCCAGCg                                                               >  2:519153/1‑135 (MQ=255)
                                                                         gAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACa                                                   <  1:717847/139‑1 (MQ=255)
                                                                                 gAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATa                                                                                                                 <  2:85508/69‑1 (MQ=255)
                                                                                 gAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATa                                                                                                                 >  1:85508/1‑69 (MQ=255)
                                                                                 gAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGa                                           <  1:436531/139‑1 (MQ=255)
                                                                                 gAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGa                                           >  1:95738/1‑139 (MQ=255)
                                                                                   aCATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACa                                                                                   <  1:203180/97‑1 (MQ=255)
                                                                                   aCATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACa                                                                                   >  2:203180/1‑97 (MQ=255)
                                                                                    cATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCa                                                                  <  1:246914/113‑1 (MQ=255)
                                                                                    cATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCa                                                                  >  2:246914/1‑113 (MQ=255)
                                                                                      tCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAg                                      >  1:750101/1‑139 (MQ=255)
                                                                                      tCCAAATCAAAAGCAATACTAAATAGAGCGCACCTAACAGGATAGCCCAGTTTTTACCTAAATAACAGCTAAAAATGCTAGTGACATCTTTACATTTTTTTGATTGTGCCAGCTTATGAATTAACTATCGCTGAAACaa                                      >  1:730453/1‑138 (MQ=255)
                                                                                               ccAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGc                             >  2:161614/1‑139 (MQ=255)
                                                                                                   cATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCggg                         <  1:442896/139‑1 (MQ=255)
                                                                                                   cATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCggg                         <  2:751646/139‑1 (MQ=255)
                                                                                                     ttACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTa                       >  1:388350/1‑139 (MQ=255)
                                                                                                        cGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAAcc                    <  1:161614/139‑1 (MQ=255)
                                                                                                             tAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCg                                                                                                             >  2:55093/1‑45 (MQ=255)
                                                                                                             tAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCg                                                                                                             <  1:55093/45‑1 (MQ=255)
                                                                                                             tAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGc                             <  1:749357/125‑1 (MQ=255)
                                                                                                             tAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGc                             >  2:749357/1‑125 (MQ=255)
                                                                                                                agCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAAt                                                 >  2:582443/1‑102 (MQ=255)
                                                                                                                agCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAAt                                                 <  1:582443/102‑1 (MQ=255)
                                                                                                                agCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCg            <  2:472943/139‑1 (MQ=255)
                                                                                                                agCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCg            >  2:77688/1‑139 (MQ=255)
                                                                                                                  cgcACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAAc                                        >  2:182573/1‑109 (MQ=255)
                                                                                                                  cgcACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAAc                                        <  1:182573/109‑1 (MQ=255)
                                                                                                                  cgcACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGAt          <  2:95738/139‑1 (MQ=255)
                                                                                                                     aCCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATgag       <  2:227722/139‑1 (MQ=255)
                                                                                                                      ccTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCt                                                                                        >  2:416979/1‑57 (MQ=255)
                                                                                                                      ccTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCt                                                                                        <  1:416979/57‑1 (MQ=255)
                                                                                                                          aCAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCaa  <  2:388350/139‑1 (MQ=255)
                                                                                                                          aCAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCaa  <  1:415696/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                   
ACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAA  >  NC_000913/3257546‑3257806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A