Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
757 |
38.1 |
1607544 |
96.5% |
1551279 |
117.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,815,917 |
Δ1 bp |
pseudogene (633/669 nt) |
rph ← |
ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,815,917 | 0 | A | . | 100.0%
| 81.0
/ NA
| 20 | pseudogene (633/669 nt) | rph | ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (14/6); total (14/6) |
CTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCA > NC_000913/3815814‑3816050
|
cTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACt > 1:755804/1‑139 (MQ=255)
aGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatg > 1:38329/1‑139 (MQ=255)
aaaaaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCg > 2:22270/1‑139 (MQ=255)
cAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCAc < 1:22270/139‑1 (MQ=255)
cgtcgtTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt > 2:795496/1‑139 (MQ=255)
gTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg > 2:730788/1‑115 (MQ=255)
gTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg < 1:730788/115‑1 (MQ=255)
aCAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCg > 2:272269/1‑139 (MQ=255)
gattcgattcCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCagag > 2:98422/1‑139 (MQ=255)
ttcgattcCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc > 2:324496/1‑92 (MQ=255)
ttcgattcCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc < 1:324496/92‑1 (MQ=255)
tcgattcCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGt > 2:175880/1‑113 (MQ=255)
tcgattcCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGt < 1:175880/113‑1 (MQ=255)
cgattcCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCtt < 2:38329/139‑1 (MQ=255)
cccTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt > 2:385099/1‑95 (MQ=255)
cccTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt < 1:385099/95‑1 (MQ=255)
cTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTAt > 2:225236/1‑139 (MQ=255)
tCGGGCCAGAGCCAACAAGATGAGTAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTAtt > 2:734028/1‑139 (MQ=255)
tAGCTCTTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGt > 1:420454/1‑139 (MQ=255)
ctctTC‑TGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTca > 2:455464/1‑139 (MQ=255)
|
CTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCA > NC_000913/3815814‑3816050
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
