Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,898,149	C→T	S43L (TCG→TTG)	cbrC →	UPF0167 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,898,149	0	C	T	100.0%	201.2 / NA	63	S43L (TCG→TTG)	cbrC	UPF0167 family protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (34/29); total (34/29)

TGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGA  >  NC_000913/3898015‑3898275

tGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGt                                                                                                                            <  1:416511/139‑1 (MQ=255)

tGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGt                                                                                                                            <  2:303660/139‑1 (MQ=255)

        tGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTgg                                                                                                                              <  1:552338/129‑1 (MQ=255)

        tGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTgg                                                                                                                              >  2:552338/1‑129 (MQ=255)

              aaaaTATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGt                                                                                                              >  2:250854/1‑139 (MQ=255)

                 atatCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTtga                                                                                                           <  1:223746/139‑1 (MQ=255)

                   atCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTtgatg                                                                                                         <  2:753626/139‑1 (MQ=255)

                        gCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGtt                                                                                                    >  2:712761/1‑139 (MQ=255)

                          cGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGa                                                                                                  >  1:735626/1‑139 (MQ=255)

                          cGTTACACCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGa                                                                                                  >  1:679062/1‑139 (MQ=255)

                           gTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGaa                                                                                                 <  2:546419/139‑1 (MQ=255)

                                cccAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtct                                                                                            <  2:28271/139‑1 (MQ=255)

                                  cAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtct                                                                                            <  2:179343/137‑1 (MQ=255)

                                  cAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtct                                                                                            >  1:179343/1‑137 (MQ=255)

                                  cAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATctct                                                                                          <  2:12372/139‑1 (MQ=255)

                                    aTTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGt                                                                                        <  1:712761/139‑1 (MQ=255)

                                    aTTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGt                                                                                        >  2:139483/1‑139 (MQ=255)

                                           tatCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTCAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCgtggt                                                                                 >  1:764018/1‑139 (MQ=255)

                                              catcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGt                                                                                                                            >  1:24302/1‑93 (MQ=255)

                                              catcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGt                                                                                                                            <  2:24302/93‑1 (MQ=255)

                                               atcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTAt                                                                             >  1:22487/1‑139 (MQ=255)

                                               atcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTAt                                                                             >  1:686711/1‑139 (MQ=255)

                                                tcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTcc                                                                                      >  1:352895/1‑129 (MQ=255)

                                                tcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTcc                                                                                      <  2:352895/129‑1 (MQ=255)

                                                tcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCgtggt                                                                                 >  2:527798/1‑134 (MQ=255)

                                                tcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCgtggt                                                                                 <  1:527798/134‑1 (MQ=255)

                                                tcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTAtt                                                                            >  2:571783/1‑139 (MQ=255)

                                                   cAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCg                                                                         <  2:114622/139‑1 (MQ=255)

                                                         aCTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGt                                                                   >  1:693632/1‑139 (MQ=255)

                                                           tGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGt                                                                                                                            >  2:791085/1‑80 (MQ=255)

                                                           tGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGt                                                                                                                            <  1:791085/80‑1 (MQ=255)

                                                           tGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTc                                                                 >  1:152444/1‑139 (MQ=255)

                                                                 cAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTctgctgc                                                           >  1:203096/1‑139 (MQ=255)

                                                                            gAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTg                                                >  2:262670/1‑139 (MQ=255)

                                                                                aGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAgg                                            <  1:250854/139‑1 (MQ=255)

                                                                                  gATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTa                                          >  1:117404/1‑139 (MQ=255)

                                                                                            tAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTtgatga                                                                                                        <  2:506933/67‑1 (MQ=255)

                                                                                            tAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTtgatga                                                                                                        >  1:506933/1‑67 (MQ=255)

                                                                                            tAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAg                                                                                                      <  2:412124/69‑1 (MQ=255)

                                                                                            tAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAg                                                                                                      >  1:412124/1‑69 (MQ=255)

                                                                                                gTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgatgat                            <  2:679062/139‑1 (MQ=255)

                                                                                                  gCGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGc                          <  1:49374/139‑1 (MQ=255)

                                                                                                   cGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGcc                         <  2:693632/139‑1 (MQ=255)

                                                                                                   cGATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGcc                         <  2:686711/139‑1 (MQ=255)

                                                                                                    gATTGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCa                        >  2:286839/1‑139 (MQ=255)

                                                                                                      ttGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTtgatga                                                                                                        >  2:275657/1‑57 (MQ=255)

                                                                                                      ttGCTGTGAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTtgatga                                                                                                        <  1:275657/57‑1 (MQ=255)

                                                                                                           gtgAACAACAGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGa                 >  2:240082/1‑139 (MQ=255)

                                                                                                                   caGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTg         >  1:763537/1‑139 (MQ=255)

                                                                                                                   caGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTg         >  2:723135/1‑139 (MQ=255)

                                                                                                                   caGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTg         <  2:203096/139‑1 (MQ=255)

                                                                                                                   caGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTg         >  2:63659/1‑139 (MQ=255)

                                                                                                                   caGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTg         >  2:479970/1‑139 (MQ=255)

                                                                                                                   caGACGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTg         >  2:397144/1‑139 (MQ=255)

                                                                                                                      aCGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGAc                                                                      >  2:442326/1‑75 (MQ=255)

                                                                                                                      aCGTCAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGAc                                                                      <  1:442326/75‑1 (MQ=255)

                                                                                                                          cAGTTTATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGa  <  1:262670/139‑1 (MQ=255)

                                                                                                                             tttATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGa                                                                       <  2:39000/67‑1 (MQ=255)

                                                                                                                             tttATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGa                                                                       >  1:39000/1‑67 (MQ=255)

                                                                                                                             tttATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgat                               <  2:215445/107‑1 (MQ=255)

                                                                                                                             tttATTACTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGgat                               >  1:215445/1‑107 (MQ=255)

                                                                                                                                  taCTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTcc                                                                                      >  2:64279/1‑47 (MQ=255)

                                                                                                                                  taCTTGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTcc                                                                                      <  1:64279/47‑1 (MQ=255)

TGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGA  >  NC_000913/3898015‑3898275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A