Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,658,893	G→A	176 (TAG→TAA)	gadE →	gad regulon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,658,893	0	G	A	99.4%	552.8 / NA	168	176 (TAG→TAA)	gadE	gad regulon transcriptional activator
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (78/89); minor base C (1/0); total (79/89)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

CACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAAC  >  NC_000913/3658773‑3659027

  cGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGaa                                                                                                                    >  1:766646/1‑139 (MQ=255)

               tatTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                       >  2:522909/1‑139 (MQ=255)

                  ttCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATg                                                                                                    <  2:1602469/139‑1 (MQ=255)

                    cGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                                  >  1:1160322/1‑139 (MQ=255)

                       aacaaacaTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                                  <  1:880210/136‑1 (MQ=255)

                       aacaaacaTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                                  >  2:880210/1‑136 (MQ=255)

                        acaaacaTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAgg                                                                                              <  2:766646/139‑1 (MQ=255)

                                     aTTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTg                                                                                 <  1:1018117/139‑1 (MQ=255)

                                       tCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAt                                                                               >  1:1566923/1‑139 (MQ=255)

                                        cAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                                  >  1:886344/1‑119 (MQ=255)

                                        cAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAtt                                                                              >  1:1554518/1‑139 (MQ=255)

                                            aaTTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCGACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAt                                                                          <  1:1001825/139‑1 (MQ=255)

                                            aaTTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAt                                                                          <  1:161600/139‑1 (MQ=255)

                                              ttCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTa                                                                        >  1:524097/1‑139 (MQ=255)

                                                  aCATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTAcccc                                                                    <  1:1996568/139‑1 (MQ=255)

                                                    aTTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCg                                                                  <  2:290735/139‑1 (MQ=255)

                                                       aTGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTg                                                               <  2:544706/139‑1 (MQ=255)

                                                         gTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTGATTACCCCCGGTGAt                                                             <  1:967003/139‑1 (MQ=255)

                                                             aaGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGa                                                                                >  1:723334/1‑116 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCaa                                                                                                                         >  1:1697870/1‑73 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCaa                                                                                                                         <  2:1697870/73‑1 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                       >  2:1944136/1‑91 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                       <  1:1944136/91‑1 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                            <  2:648014/86‑1 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                            >  1:1745986/1‑86 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                            <  2:1745986/86‑1 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                            <  2:1723595/86‑1 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGa                                                                                <  2:79477/114‑1 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAtt                                                                         <  2:227834/121‑1 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAtt                                                                         >  1:227834/1‑121 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                             >  2:1960774/1‑133 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTaa                                                       >  1:43329/1‑139 (MQ=255)

                                                               gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTaa                                                       >  2:742341/1‑139 (MQ=255)

                                                                  gAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAgg                                                    <  1:692909/139‑1 (MQ=255)

                                                                   aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACt                                                                                                                       >  2:1979466/1‑71 (MQ=255)

                                                                   aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGga                                                   >  2:1904482/1‑139 (MQ=255)

                                                                    aaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATg                                                                                    >  1:1099898/1‑105 (MQ=255)

                                                                    aaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATg                                                                                    <  2:1099898/105‑1 (MQ=255)

                                                                     aCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGgaga                                                 >  1:709867/1‑139 (MQ=255)

                                                                         aGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAt                                                                               >  1:1775858/1‑105 (MQ=255)

                                                                          gACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTg                                                               <  2:756487/120‑1 (MQ=255)

                                                                              tATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACa                                                                                                                                >  2:1458717/1‑51 (MQ=255)

                                                                              tATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACa                                                                                                                                <  1:1458717/51‑1 (MQ=255)

                                                                               aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTACTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAtt                                                                         >  2:1022886/1‑105 (MQ=255)

                                                                               aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAtt                                                                         <  1:1022886/105‑1 (MQ=255)

                                                                               aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                             <  2:1542472/117‑1 (MQ=255)

                                                                                  aGCGACATCGTCACCCTGGGAATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACt                                    >  1:892638/1‑139 (MQ=255)

                                                                                     gACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCTACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                                 >  2:1021340/1‑139 (MQ=255)

                                                                                     gACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAt                                                                               <  2:1991788/93‑1 (MQ=255)

                                                                                     gACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAt                                                                               >  1:1991788/1‑93 (MQ=255)

                                                                                     gACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                                 <  1:583985/139‑1 (MQ=255)

                                                                                      aCATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTAt                                >  1:1959489/1‑139 (MQ=255)

                                                                                       cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTAtt                               <  1:1904482/139‑1 (MQ=255)

                                                                                        aTCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTg                                                               <  2:48398/106‑1 (MQ=255)

                                                                                        aTCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTg                                                               >  1:48398/1‑106 (MQ=255)

                                                                                        aTCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTc                              >  2:840967/1‑139 (MQ=255)

                                                                                           gTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                            <  2:1321769/58‑1 (MQ=255)

                                                                                           gTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTcccc                           <  2:1598088/139‑1 (MQ=255)

                                                                                              aCCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTgg                        <  2:1940742/139‑1 (MQ=255)

                                                                                                ccTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTa                      <  2:1566923/139‑1 (MQ=255)

                                                                                                 cTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTAt                     <  1:755718/139‑1 (MQ=255)

                                                                                                 cTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTAt                     <  2:709867/139‑1 (MQ=255)

                                                                                                    ggTATCACATCTTATTTTTAATCAGGACATACGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTAtgtg                  <  2:1830608/139‑1 (MQ=255)

                                                                                                    ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                                  <  2:1269786/59‑1 (MQ=255)

                                                                                                    ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCt                                                                                  >  2:13905/1‑75 (MQ=255)

                                                                                                    ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCt                                                                                  <  1:13905/75‑1 (MQ=255)

                                                                                                    ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAtt                                                                         <  2:1879271/84‑1 (MQ=255)

                                                                                                    ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                             <  1:1291310/96‑1 (MQ=255)

                                                                                                    ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc                            <  1:336974/129‑1 (MQ=255)

                                                                                                    ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc                            >  1:572142/1‑129 (MQ=255)

                                                                                                    ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc                            >  2:336974/1‑129 (MQ=255)

                                                                                                    ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTAtgtg                  >  1:1875863/1‑139 (MQ=255)

                                                                                                    ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTAt                     >  2:1474865/1‑136 (MQ=255)

                                                                                                    ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTCGCATGAc                                                                                                  >  1:1269786/1‑59 (MQ=255)

                                                                                                     gTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATg                                                                                    >  1:497584/1‑72 (MQ=255)

                                                                                                     gTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTAccc                                                                     <  2:1506914/87‑1 (MQ=255)

                                                                                                     gTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTAccc                                                                     >  1:1506914/1‑87 (MQ=255)

                                                                                                      tATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTg                                                               <  2:362873/92‑1 (MQ=255)

                                                                                                        tCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATc              <  1:356530/139‑1 (MQ=255)

                                                                                                         cacaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTAcccc                                                                    <  2:1172706/84‑1 (MQ=255)

                                                                                                         cacaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTa                                                        <  2:1537070/96‑1 (MQ=255)

                                                                                                         cacaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATcc             >  2:533757/1‑139 (MQ=255)

                                                                                                         cacaTCTTATTTTTAATCAGAACATAAGCAACTGAAATTGATGGCTGGCATGACGATGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTa                                                        >  1:1537070/1‑96 (MQ=255)

                                                                                                          acaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATg                                                                                    <  1:1976617/67‑1 (MQ=255)

                                                                                                          acaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATcca            >  1:996291/1‑139 (MQ=255)

                                                                                                           caTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATccac           <  2:892638/139‑1 (MQ=255)

                                                                                                              cTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                       <  1:13695/44‑1 (MQ=255)

                                                                                                              cTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                       >  2:13695/1‑44 (MQ=255)

                                                                                                              cTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                            <  1:1773274/39‑1 (MQ=255)

                                                                                                              cTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                            >  2:1773274/1‑39 (MQ=255)

                                                                                                              cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTg                                                               >  1:1476856/1‑84 (MQ=255)

                                                                                                              cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGa                                      >  1:1706384/1‑109 (MQ=255)

                                                                                                              cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                                 >  2:1862106/1‑114 (MQ=255)

                                                                                                              cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTa                >  2:1179867/1‑131 (MQ=255)

                                                                                                              cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAg        >  2:957041/1‑139 (MQ=255)

                                                                                                              cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCACATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTa                <  1:1179867/131‑1 (MQ=255)

                                                                                                                tATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTaaaa                                         >  1:1429714/1‑104 (MQ=255)

                                                                                                                tATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc                            >  2:462154/1‑117 (MQ=255)

                                                                                                                tATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTa      <  1:781557/139‑1 (MQ=255)

                                                                                                                    tttAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAAc  <  2:703241/139‑1 (MQ=255)

CACGTCGACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAAC  >  NC_000913/3658773‑3659027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A