Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,740,926 |
G→A |
E408K (GAG→AAG) |
avtA → |
valine‑‑pyruvate aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,740,926 | 0 | G | A | 100.0%
| 67.9
/ NA
| 20 | E408K (GAG→AAG) | avtA | valine‑‑pyruvate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (7/13); total (7/13) |
TACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTC > NC_000913/3740850‑3741000
|
tACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAAAGAt < 1:669628/81‑1 (MQ=255)
tCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAAAGATAGAAag < 1:669629/81‑1 (MQ=255)
aTGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTgg > 1:669656/1‑81 (MQ=255)
tGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTggg < 1:669630/81‑1 (MQ=255)
aaCTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGt < 1:669631/81‑1 (MQ=255)
cGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCAc < 1:669632/79‑1 (MQ=255)
agagCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAAtt < 2:669633/81‑1 (MQ=255)
ggAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGa > 2:669653/1‑81 (MQ=255)
ggAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGa > 1:669654/1‑81 (MQ=255)
gaAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgc < 1:669634/81‑1 (MQ=255)
aaaaTTGAGGCGGGGGTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcg > 1:669658/1‑81 (MQ=255)
ttGAGGCGGGGGTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTAc < 2:669635/81‑1 (MQ=255)
gAGGCGGGGGTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGc > 2:669661/1‑81 (MQ=255)
cGGGGGTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTAt > 2:669657/1‑81 (MQ=255)
gggggTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATc < 2:669636/81‑1 (MQ=255)
ggggTGAAGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCa < 2:669637/81‑1 (MQ=255)
aaGATTCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCt < 1:669638/80‑1 (MQ=255)
ttCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAt > 2:669655/1‑80 (MQ=255)
ttCTGGCGGAAAAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATg < 2:669639/81‑1 (MQ=255)
gCGGAAAAGATAGAAAGAGCCTGTGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTc < 1:669640/81‑1 (MQ=255)
|
TACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTC > NC_000913/3740850‑3741000
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
