Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,822,364 |
G→A |
A87T (GCT→ACT) |
rpoZ → |
RNA polymerase subunit omega |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,822,364 | 0 | G | A | 89.2%
| 99.7
/ 4.1
| 37 | A87T (GCT→ACT) | rpoZ | RNA polymerase subunit omega |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base A (22/11); total (25/12) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 |
AACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTC > NC_000913/3822286‑3822433
|
aacCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACt > 2:685308/1‑81 (MQ=255)
aacCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACt > 1:685297/1‑81 (MQ=255)
acCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCTGCTGAATTACAAGCCGTTACCGCTATTACt > 2:685305/1‑80 (MQ=255)
aGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAg > 1:685274/1‑81 (MQ=255)
aTCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGgt < 2:685275/81‑1 (MQ=255)
ccTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGgtcg > 2:685314/1‑81 (MQ=255)
tCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGgtcgtc > 1:685309/1‑81 (MQ=255)
cGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGtcgtcg > 2:685318/1‑81 (MQ=255)
gACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGgtcgtc < 1:685276/79‑1 (MQ=255)
gACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGgtcgtc < 1:685277/79‑1 (MQ=255)
gACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTcgtcgt > 2:685310/1‑81 (MQ=255)
gACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTcgtcgt > 1:685341/1‑81 (MQ=255)
gTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTaa > 1:685330/1‑81 (MQ=255)
gTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTaa > 1:685331/1‑81 (MQ=255)
gcgAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTTAATTACAAGCCGTTATCGCTATTACTGAAGGTTGTAGTTAATca > 2:685338/1‑80 (MQ=255)
gcgAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATcac < 1:685278/81‑1 (MQ=255)
gcgAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATca > 1:685324/1‑80 (MQ=255)
cgAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATcac > 2:685333/1‑80 (MQ=255)
gAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATcaca > 2:685321/1‑80 (MQ=255)
ccAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCgg < 1:685279/81‑1 (MQ=255)
cAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCggg > 2:685316/1‑81 (MQ=255)
cAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGccc > 1:685336/1‑81 (MQ=255)
aaGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGt < 1:685280/81‑1 (MQ=255)
aGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGt > 2:685288/1‑81 (MQ=255)
gAAGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGttt < 1:685281/81‑1 (MQ=255)
aaGCCGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTg < 1:685282/81‑1 (MQ=255)
cGCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAg < 1:685283/81‑1 (MQ=255)
gCTGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGc > 2:685343/1‑81 (MQ=255)
tGAATTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGCATCTGTTTGAAAGCCt > 1:685339/1‑81 (MQ=255)
aaTTACAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCt < 1:685284/79‑1 (MQ=255)
ttACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAAt > 2:685322/1‑81 (MQ=255)
cAAGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCa < 1:685285/80‑1 (MQ=255)
aaGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAAc > 2:685328/1‑81 (MQ=255)
aaGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAAc > 2:685327/1‑81 (MQ=255)
aaGCCGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAAc > 2:685329/1‑81 (MQ=255)
ccGTTACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGa > 2:685334/1‑81 (MQ=255)
ttACCGCTATTACTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTc < 1:685286/81‑1 (MQ=255)
|
AACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTC > NC_000913/3822286‑3822433
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
