Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,879,342 |
C→T |
D260N (GAT→AAT) |
gyrB ← |
DNA gyrase subunit B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,879,342 | 0 | C | T | 100.0%
| 96.7
/ NA
| 28 | D260N (GAT→AAT) | gyrB | DNA gyrase subunit B |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/18); total (10/18) |
GAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGC > NC_000913/3879265‑3879418
|
gAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGtt > 1:697642/1‑81 (MQ=255)
tGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTg > 2:697658/1‑81 (MQ=255)
ccAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGc > 2:697640/1‑80 (MQ=255)
gTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGc > 1:697656/1‑81 (MQ=255)
aGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCa < 2:697603/80‑1 (MQ=255)
gccgTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGAc > 2:697653/1‑81 (MQ=255)
gccgTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGAc < 2:697604/81‑1 (MQ=255)
cgTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGc > 2:697654/1‑81 (MQ=255)
gTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGc > 2:697649/1‑80 (MQ=255)
ggAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCt < 2:697605/81‑1 (MQ=255)
aTGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCtttt < 1:697607/81‑1 (MQ=255)
aTGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCtttt < 1:697606/81‑1 (MQ=255)
tgttgtTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCttttt < 2:697608/81‑1 (MQ=255)
gttgttGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCttttt > 2:697665/1‑80 (MQ=255)
ttgGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGt < 1:697610/80‑1 (MQ=255)
ttgGTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTg < 1:697609/81‑1 (MQ=255)
gTAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGgag < 2:697611/81‑1 (MQ=255)
tAAAGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGgaga < 2:697612/81‑1 (MQ=255)
aaGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGaa < 1:697613/80‑1 (MQ=255)
aGCAGTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAg > 1:697659/1‑80 (MQ=255)
gTAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTagaa < 1:697614/81‑1 (MQ=255)
tAGATGTTTTCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTagaag < 2:697615/81‑1 (MQ=255)
ttttCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTc < 1:697616/81‑1 (MQ=255)
tCCTGGAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCgg < 1:697617/80‑1 (MQ=255)
ggAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGa > 2:697655/1‑81 (MQ=255)
gAAGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTgg < 1:697618/79‑1 (MQ=255)
aaGCCATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATc < 2:697619/81‑1 (MQ=255)
ccATTGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGc < 2:697620/81‑1 (MQ=255)
|
GAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGC > NC_000913/3879265‑3879418
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
