Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,907,030 |
C→A |
S183S (TCG→TCT) |
phoU ← |
negative regulator of the pho regulon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,907,030 | 0 | C | A | 100.0%
| 130.2
/ NA
| 37 | S183S (TCG→TCT) | phoU | negative regulator of the pho regulon |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (25/12); total (25/12) |
AATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGA > NC_000913/3906952‑3907105
|
aaTATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACgaga < 2:703062/81‑1 (MQ=255)
ttCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGTAGTAAGTACGCTCGGAATGGTACGAGAATCt > 2:703098/1‑81 (MQ=255)
ttCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCt < 1:703063/81‑1 (MQ=255)
cTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCtt < 1:703064/80‑1 (MQ=255)
cAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCcat > 2:703085/1‑80 (MQ=255)
gCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCcatcat < 2:703065/81‑1 (MQ=255)
cGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATg < 1:703066/81‑1 (MQ=255)
ggTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATg > 2:703067/1‑80 (MQ=255)
ccAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGt > 1:703092/1‑80 (MQ=255)
ccAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGt > 1:703094/1‑80 (MQ=255)
tACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGtcat > 1:703116/1‑79 (MQ=255)
cGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAg < 1:703068/80‑1 (MQ=255)
gTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGtt > 1:703096/1‑81 (MQ=255)
aTAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACg > 1:703100/1‑80 (MQ=255)
aCGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACaa > 2:703107/1‑80 (MQ=255)
cgcgcgCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATa > 1:703101/1‑81 (MQ=255)
cgcgcgCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATa > 1:703117/1‑81 (MQ=255)
gcgcgcAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATAc < 1:703069/81‑1 (MQ=255)
gcAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTc > 1:703090/1‑81 (MQ=255)
aGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCg > 2:703115/1‑80 (MQ=255)
aGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCg > 1:703108/1‑80 (MQ=255)
cAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTAGTc > 2:703099/1‑81 (MQ=255)
aGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTcc < 1:703070/81‑1 (MQ=255)
gcAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGa > 1:703103/1‑81 (MQ=255)
gaagtaagtaCGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGa > 2:703102/3‑81 (MQ=255)
agtaCGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGAc < 2:703071/80‑1 (MQ=255)
gtaCGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACtt > 2:703110/1‑81 (MQ=255)
taCGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACttt < 1:703072/81‑1 (MQ=255)
cGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACttttt > 2:703112/1‑81 (MQ=255)
cGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACttttt < 2:703073/81‑1 (MQ=255)
cGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACttttt > 2:703074/1‑81 (MQ=255)
gCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACtttttt < 1:703075/81‑1 (MQ=255)
gCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACttttt > 1:703119/1‑80 (MQ=255)
ggAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCt > 2:703104/1‑81 (MQ=255)
aaTGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTc < 2:703076/81‑1 (MQ=255)
gTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGa > 1:703106/1‑81 (MQ=255)
gTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGa > 1:703111/1‑81 (MQ=255)
gTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGa > 1:703095/1‑81 (MQ=255)
|
AATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGA > NC_000913/3906952‑3907105
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
