Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,921,309 |
A→T |
I248N (ATC→AAC) |
atpB ← |
ATP synthase Fo complex subunit a |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,921,309 | 0 | A | T | 100.0%
| 135.9
/ NA
| 39 | I248N (ATC→AAC) | atpB | ATP synthase Fo complex subunit a |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (18/21); total (18/21) |
GTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGA > NC_000913/3921231‑3921383
|
gTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTc < 2:706481/81‑1 (MQ=255)
aaaTTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAg > 2:706517/1‑81 (MQ=255)
aaTTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAgg < 1:706482/81‑1 (MQ=255)
ttAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGAt < 1:706483/81‑1 (MQ=255)
tGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTgg < 2:706484/81‑1 (MQ=255)
tcttcAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGaa > 1:706548/1‑80 (MQ=255)
cAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATgg > 1:706526/1‑81 (MQ=255)
cAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATg < 1:706485/80‑1 (MQ=255)
aGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATgg < 1:706486/80‑1 (MQ=255)
aCGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGcc > 1:706529/1‑80 (MQ=255)
aCGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGcc > 2:706524/1‑80 (MQ=255)
cGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCa < 1:706487/81‑1 (MQ=255)
gCCATCGACAGATAGCCGATCGTCAGAACCATGAAGCTGAAGGCTTGCAGCGTAATGTTCAGTATGTGGTAAATGGCCCAc > 2:706539/1‑81 (MQ=255)
gCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCAc > 1:706541/1‑81 (MQ=255)
gCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCAc < 1:706488/81‑1 (MQ=255)
aTCGACAGATAGACGATCGTCAGAATCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACgg > 2:706537/1‑80 (MQ=255)
aTCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACgg > 1:706513/1‑80 (MQ=255)
aTCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACgg > 2:706492/1‑80 (MQ=255)
aTCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGc < 2:706489/81‑1 (MQ=255)
aTCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGc < 2:706491/81‑1 (MQ=255)
aTCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGc < 2:706490/81‑1 (MQ=255)
tCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGTca > 2:706542/1‑81 (MQ=255)
tCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGca < 2:706493/81‑1 (MQ=255)
gACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGcac < 1:706494/80‑1 (MQ=255)
cAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACAtt < 2:706495/81‑1 (MQ=255)
cAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACAtt < 1:706496/81‑1 (MQ=255)
aGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTc < 1:706497/81‑1 (MQ=255)
aTAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAg > 2:706534/1‑81 (MQ=255)
gATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCa < 2:706498/81‑1 (MQ=255)
cGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACtg > 1:706550/1‑81 (MQ=255)
tCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACtgt > 2:706532/1‑80 (MQ=255)
aGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGac > 1:706533/1‑81 (MQ=255)
aaCCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGacca > 2:706540/1‑81 (MQ=255)
ccATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGaccac > 1:706535/1‑80 (MQ=255)
tgaagatgaagGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCAACTGTGACCACCACg < 2:706499/81‑1 (MQ=255)
agatgaagGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGc > 1:706531/1‑80 (MQ=255)
tgaagGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACa < 1:706500/81‑1 (MQ=255)
cAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCaa < 2:706501/80‑1 (MQ=255)
gTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGa < 2:706502/81‑1 (MQ=255)
|
GTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGA > NC_000913/3921231‑3921383
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
