Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,933,275 |
A→G |
intergenic (+91/‑76) |
kup → / → rbsD |
K(+):H(+) symporter Kup/D‑ribose pyranase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,933,275 | 0 | A | G | 100.0%
| 62.7
/ NA
| 19 | intergenic (+91/‑76) | kup/rbsD | K(+):H(+) symporter Kup/D‑ribose pyranase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/7); total (12/7) |
TTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAA > NC_000913/3933199‑3933351
|
tttCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTGACGt > 1:709064/1‑81 (MQ=255)
tcATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTGACGTCACGa > 1:709095/1‑81 (MQ=255)
aaGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTGACGTCACGATGGt > 2:709093/1‑81 (MQ=255)
gCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTGACGTCACGATGGTTTTccc < 1:709065/81‑1 (MQ=255)
tCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTGACGTCACGATGGTTTTCCCAACtcagtcag > 1:709086/1‑80 (MQ=255)
tGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGAt > 1:709087/1‑80 (MQ=255)
aTGTAAAACGTTTCGAGGTTGATCACATTTCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTc < 2:709066/81‑1 (MQ=255)
tAAAACGTTTCGAGGTTGATCACATTTCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGc > 2:709092/1‑81 (MQ=255)
aaaCGTTTCGAGGTTGATCACATTTCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCg > 1:709090/1‑80 (MQ=255)
gTTTCGAGGTTGATCACATTTCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACg > 1:709103/1‑81 (MQ=255)
cGAGGTTGATCACATTTCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTc > 2:709109/1‑81 (MQ=255)
gAGGTTGATCACATTTCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTc < 2:709067/80‑1 (MQ=255)
gAGGTTGATCACATTTCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTc < 2:709069/80‑1 (MQ=255)
gAGGTTGATCACATTTCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTc < 1:709068/80‑1 (MQ=255)
gTTGATCACATTTCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCt > 2:709091/1‑80 (MQ=255)
gATCACATTTCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATg > 2:709097/1‑81 (MQ=255)
tttCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGaaaa < 2:709070/81‑1 (MQ=255)
tttCCGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGaaaa < 2:709071/81‑1 (MQ=255)
ccGTGACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGaaaaaaa > 1:709098/1‑81 (MQ=255)
|
TTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAA > NC_000913/3933199‑3933351
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
