Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,181,145 |
T→C |
intergenic (+220/‑100) |
rplL → / → rpoB |
50S ribosomal subunit protein L12/RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,181,145 | 0 | T | C | 100.0%
| 122.4
/ NA
| 35 | intergenic (+220/‑100) | rplL/rpoB | 50S ribosomal subunit protein L12/RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/16); total (19/16) |
GACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGA > NC_000913/4181069‑4181220
|
gacTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCg > 1:762280/1‑81 (MQ=255)
acTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCgg < 1:762229/81‑1 (MQ=255)
tAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGa > 2:762268/1‑79 (MQ=255)
taCTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAgtgt < 2:762230/80‑1 (MQ=255)
taCTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGtt < 1:762231/81‑1 (MQ=255)
aCTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTc > 2:762265/1‑81 (MQ=255)
cTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTcc < 1:762233/81‑1 (MQ=255)
cTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTc < 1:762232/80‑1 (MQ=255)
tGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCa > 2:762270/1‑81 (MQ=255)
gACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCAttt > 1:762282/1‑81 (MQ=255)
gACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCAttt > 2:762276/1‑81 (MQ=255)
gACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCAttt > 2:762275/1‑81 (MQ=255)
cAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTc < 1:762234/81‑1 (MQ=255)
ggACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCg > 1:762285/1‑81 (MQ=255)
cGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTc < 1:762235/81‑1 (MQ=255)
gTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTc > 2:762286/1‑80 (MQ=255)
gTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTc > 2:762273/1‑80 (MQ=255)
cGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTcaac < 1:762236/80‑1 (MQ=255)
tgtgTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACaaaa > 2:762278/1‑79 (MQ=255)
gtgtAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAg > 2:762289/1‑81 (MQ=255)
tgtAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATa < 1:762237/79‑1 (MQ=255)
aaaTCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGtt > 2:762305/1‑81 (MQ=255)
aTCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTg > 2:762274/1‑80 (MQ=255)
aaaTGGTTTAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTg < 2:762238/80‑1 (MQ=255)
tAAGCGTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCa < 2:762239/80‑1 (MQ=255)
gTGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGa > 2:762283/1‑80 (MQ=255)
tGATAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACa < 2:762240/81‑1 (MQ=255)
tAGCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGAt > 2:762279/1‑81 (MQ=255)
gCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATgg > 2:762277/1‑81 (MQ=255)
gCAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATg < 2:762241/80‑1 (MQ=255)
cAACAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATggg < 2:762242/81‑1 (MQ=255)
aaCAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAAGAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGt < 2:762243/81‑1 (MQ=255)
aCAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTc > 2:762288/1‑81 (MQ=255)
cAGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTc > 2:762294/1‑80 (MQ=255)
aGGCACTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGa < 1:762244/81‑1 (MQ=255)
|
GACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGA > NC_000913/4181069‑4181220
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
