Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,305,722 |
C→A |
A292A (GCG→GCT) |
yjcS ← |
alkyl sulfatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,305,722 | 0 | C | A | 100.0%
| 139.9
/ NA
| 41 | A292A (GCG→GCT) | yjcS | alkyl sulfatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (22/19); total (22/19) |
GTTGTGCAGGGTATGCGTGGCGTTCTCGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGCGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGT > NC_000913/4305644‑4305799
|
gTTGTGCAGGGTATGCGTGGCGTTCTCGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGgagc < 2:789172/81‑1 (MQ=255)
cAGGGTATGCGTGGCGTTCTCGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGc > 2:789235/1‑80 (MQ=255)
aGGGTATGCGTGGCGTTCTCGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTa > 2:789208/1‑81 (MQ=255)
tATGCGTGGCGTTCTCGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTg < 2:789173/81‑1 (MQ=255)
tATGCGTGGCGTTCTCGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTg > 1:789237/1‑81 (MQ=255)
aTGCGTGGCGTTCTCGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTgg > 1:789206/1‑81 (MQ=255)
tGCGTGGCGTTCTCGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTggg < 2:789174/81‑1 (MQ=255)
tGCGTGGCGTTCTCGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCAGTTCGGCTGGAGCTTCGCTACCTggg < 2:789175/81‑1 (MQ=255)
gTGGCGTTCTCGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGt > 2:789229/1‑80 (MQ=255)
gCGTTCTCGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGtcat > 1:789213/1‑80 (MQ=255)
ctcGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGa < 1:789176/80‑1 (MQ=255)
cggcggTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGt > 2:789242/1‑81 (MQ=255)
ggTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCaaa > 2:789241/1‑81 (MQ=255)
acaGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCa < 2:789177/81‑1 (MQ=255)
gggCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGc < 2:789178/81‑1 (MQ=255)
gggCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGc > 1:789244/1‑81 (MQ=255)
gggCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGc > 1:789245/1‑81 (MQ=255)
tttCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTc > 2:789234/1‑81 (MQ=255)
ttCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTc > 2:789220/1‑80 (MQ=255)
ttCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTc > 1:789246/1‑80 (MQ=255)
tCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTc < 2:789179/79‑1 (MQ=255)
tCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCg < 1:789181/80‑1 (MQ=255)
aGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGAt < 1:789183/80‑1 (MQ=255)
aGGGCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATa < 1:789182/81‑1 (MQ=255)
ggCCGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAat < 1:789184/81‑1 (MQ=255)
cGGAATATAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAatcat < 1:789185/81‑1 (MQ=255)
atatAGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCAtcttc > 1:789232/1‑81 (MQ=255)
taGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTtctc < 2:789186/80‑1 (MQ=255)
taGAAGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCg > 2:789230/1‑81 (MQ=255)
aaGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCa < 2:789188/81‑1 (MQ=255)
aaGTGCATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCa < 1:789187/81‑1 (MQ=255)
cATTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCt < 1:789189/81‑1 (MQ=255)
aTTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTg < 2:789190/81‑1 (MQ=255)
aTTTCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTg < 2:789191/81‑1 (MQ=255)
tttCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGa > 2:789257/1‑81 (MQ=255)
tttCGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGa > 2:789239/1‑81 (MQ=255)
cGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGa > 1:789233/1‑81 (MQ=255)
cGGCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGa > 1:789238/1‑81 (MQ=255)
ggCTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGCCGATAATCATCTTCTCGCCAGTTCTGACGa > 2:789252/1‑80 (MQ=255)
cTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAg < 2:789192/81‑1 (MQ=255)
cTGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAg > 1:789240/1‑81 (MQ=255)
tGGAGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGt > 1:789247/1‑81 (MQ=255)
|
GTTGTGCAGGGTATGCGTGGCGTTCTCGGCGGTACACAGGGCTTTCAGGGCCGGAATATAGAAGTGCATTTCGGCTGGCGCTTCGCTACCTGGGGTCATCAGGAAGTCAAACTCCAGGCCGTCGATAATCATCTTCTCGCCAGTTCTGACGATAGT > NC_000913/4305644‑4305799
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
