Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,347,445 |
C→T |
V434I (GTC→ATC) |
dcuB ← |
anaerobic C4‑dicarboxylate transporter DcuB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,347,445 | 0 | C | T | 100.0%
| 134.0
/ NA
| 38 | V434I (GTC→ATC) | dcuB | anaerobic C4‑dicarboxylate transporter DcuB |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/12); total (26/12) |
GTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGC > NC_000913/4347369‑4347522
|
gTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCAc > 1:798267/1‑81 (MQ=255)
ccGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGa > 1:798259/1‑80 (MQ=255)
ccGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGAt < 1:798229/81‑1 (MQ=255)
ccGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGAt < 2:798230/81‑1 (MQ=255)
gcgTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGACGATGCACGATAc > 1:798256/1‑81 (MQ=255)
gcgTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATAc > 2:798257/1‑81 (MQ=255)
gcgTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATAc < 2:798231/81‑1 (MQ=255)
aGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCt > 1:798255/1‑80 (MQ=255)
gTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTc < 2:798232/80‑1 (MQ=255)
gggCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACAcc > 2:798280/1‑80 (MQ=255)
gcggcACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAAt > 1:798263/1‑81 (MQ=255)
gcACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCaa > 1:798260/1‑81 (MQ=255)
aCGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAAcc > 1:798269/1‑81 (MQ=255)
cAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCg > 2:798264/1‑81 (MQ=255)
aaaGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCgg > 1:798261/1‑81 (MQ=255)
aGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCgg > 2:798283/1‑79 (MQ=255)
aGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCa < 2:798233/81‑1 (MQ=255)
tttATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATaa < 1:798234/81‑1 (MQ=255)
tttATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATaa < 1:798235/81‑1 (MQ=255)
aGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCtgt > 1:798285/1‑81 (MQ=255)
aGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCtgt > 1:798294/1‑81 (MQ=255)
ccGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtg < 1:798236/81‑1 (MQ=255)
gTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgat > 1:798278/1‑81 (MQ=255)
gTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgat < 2:798237/81‑1 (MQ=255)
aCATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgatga > 2:798271/1‑81 (MQ=255)
aCATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgatga > 1:798286/1‑81 (MQ=255)
cATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGAc < 1:798238/81‑1 (MQ=255)
ggcgAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGc > 2:798276/1‑80 (MQ=255)
ggcgAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGc > 2:798300/1‑80 (MQ=255)
cgAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGAc < 2:798239/81‑1 (MQ=255)
cgAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGAc > 2:798281/1‑81 (MQ=255)
aGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGa < 1:798240/81‑1 (MQ=255)
ccAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGAtgtg > 2:798284/1‑81 (MQ=255)
cGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATgtgggtgg > 1:798265/1‑81 (MQ=255)
cGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATgtgggtgg > 2:798277/1‑81 (MQ=255)
gAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGggtggt > 1:798272/1‑81 (MQ=255)
gAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGggtggt > 2:798291/1‑81 (MQ=255)
aGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGc > 1:798279/1‑81 (MQ=255)
|
GTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGC > NC_000913/4347369‑4347522
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
