Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,402,976 |
G→A |
P313P (CCG→CCA) |
hflK → |
regulator of FtsH protease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,402,976 | 0 | G | A | 100.0%
| 104.5
/ NA
| 31 | P313P (CCG→CCA) | hflK | regulator of FtsH protease |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/18); total (13/18) |
GAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAA > NC_000913/4402899‑4403047
|
gAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGaa > 2:809644/1‑81 (MQ=255)
gAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGa < 1:809600/80‑1 (MQ=255)
ggCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATaa < 2:809601/81‑1 (MQ=255)
gcgcGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAAtata > 2:809641/1‑79 (MQ=255)
gcgcGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATaaa < 2:809602/81‑1 (MQ=255)
cgcgTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAg < 2:809603/81‑1 (MQ=255)
cgtgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGcc < 2:809604/81‑1 (MQ=255)
tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCcgc > 1:809632/1‑81 (MQ=255)
tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCcgc < 2:809605/81‑1 (MQ=255)
gtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGcc < 1:809606/81‑1 (MQ=255)
aaGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGaa < 1:809607/81‑1 (MQ=255)
aGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGaaa < 2:809608/81‑1 (MQ=255)
cAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACt > 2:809650/1‑81 (MQ=255)
cAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACt < 2:809609/81‑1 (MQ=255)
aGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTc > 1:809649/1‑81 (MQ=255)
cTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGt < 2:809610/81‑1 (MQ=255)
cTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGt > 2:809637/1‑81 (MQ=255)
gAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTc < 2:809611/79‑1 (MQ=255)
gAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTg > 2:809639/1‑81 (MQ=255)
aaGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGt < 2:809612/81‑1 (MQ=255)
aGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGta < 1:809613/81‑1 (MQ=255)
tCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTatatat > 2:809647/1‑81 (MQ=255)
tGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGAc > 2:809651/1‑81 (MQ=255)
tGAAGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGAc > 2:809638/1‑81 (MQ=255)
aGTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGAt > 1:809654/1‑81 (MQ=255)
gTGGCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATg < 1:809614/81‑1 (MQ=255)
ggCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATgg > 2:809640/1‑80 (MQ=255)
ggCGCGCTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATgg > 1:809646/1‑80 (MQ=255)
cgcgcTTTGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGaaa < 1:809615/81‑1 (MQ=255)
tGCTAAACTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAgtgt < 2:809616/80‑1 (MQ=255)
aCTTCTGCCAGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTaa < 2:809617/81‑1 (MQ=255)
|
GAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAA > NC_000913/4402899‑4403047
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
