Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,494,085 |
T→C |
T108A (ACA→GCA) |
idnD ← |
L‑idonate 5‑dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,494,085 | 0 | T | C | 100.0%
| 79.4
/ NA
| 23 | T108A (ACA→GCA) | idnD | L‑idonate 5‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (6/17); total (6/17) |
TCGACCATTTTATAACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTT > NC_000913/4494009‑4494159
|
tCGACCATTTTATAACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGcaca < 1:828223/80‑1 (MQ=255)
ccATTTTATAACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGAt > 1:828262/1‑81 (MQ=255)
cATTTTATAACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGAtt < 1:828224/81‑1 (MQ=255)
taACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGtt < 2:828225/80‑1 (MQ=255)
aCGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATg < 2:828226/81‑1 (MQ=255)
gTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCa < 2:828227/81‑1 (MQ=255)
aaaCCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATg < 1:828228/81‑1 (MQ=255)
caccaTCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAg > 2:828269/1‑80 (MQ=255)
aaCATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGc < 2:828229/80‑1 (MQ=255)
aCATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAg > 1:828267/1‑81 (MQ=255)
aTGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTg < 2:828230/81‑1 (MQ=255)
gAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGAc > 2:828258/1‑81 (MQ=255)
aGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGAcc < 1:828231/81‑1 (MQ=255)
ggAAATACATGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGc < 2:828232/81‑1 (MQ=255)
tGGCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAg < 1:828233/81‑1 (MQ=255)
ggCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGa > 1:828270/1‑81 (MQ=255)
gCACTGCCAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTTACCGCACGGTTTAGAc < 1:828234/81‑1 (MQ=255)
ccAAAAAAACGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGAtt < 1:828235/80‑1 (MQ=255)
aaaaaCGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATgg < 2:828236/81‑1 (MQ=255)
aaaaaCGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATgg > 2:828272/1‑81 (MQ=255)
aaaCGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCt < 2:828237/81‑1 (MQ=255)
cGCATATCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTAcc < 1:828238/81‑1 (MQ=255)
atatCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGtt < 2:828239/81‑1 (MQ=255)
tatCTGCACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGttt < 1:828240/81‑1 (MQ=255)
|
TCGACCATTTTATAACGGGTAAAACCACCATCAACATGAGGGAAATACATGGCACTGCCAAAAAAACGCATATCTGTACACTGATTCTCGTTATGTTCAATGCAGTATTTGCAGTGACCGCACGGTTTAGACGGATTAATGGCTACCGTTT > NC_000913/4494009‑4494159
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
