Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,516,866 |
G→A |
F284F (TTC→TTT) |
fecR ← |
regulator for fec operon, periplasmic |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,516,866 | 0 | G | A | 100.0%
| 100.7
/ NA
| 29 | F284F (TTC→TTT) | fecR | regulator for fec operon, periplasmic |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/11); total (18/11) |
GTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGC > NC_000913/4516788‑4516943
|
gTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGaaa < 1:832638/81‑1 (MQ=255)
tACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAAc < 2:832639/81‑1 (MQ=255)
aCCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACg > 2:832668/1‑81 (MQ=255)
aCCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACg > 2:832677/1‑81 (MQ=255)
gcgTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTcc < 1:832640/81‑1 (MQ=255)
gTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCg > 1:832667/1‑81 (MQ=255)
aaTTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCa < 1:832641/80‑1 (MQ=255)
ttttAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGcc < 2:832642/81‑1 (MQ=255)
gggAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAAc > 1:832679/1‑81 (MQ=255)
gggAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAAc > 1:832689/1‑81 (MQ=255)
gggAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAAc < 2:832643/81‑1 (MQ=255)
aaGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAAcggc < 1:832644/81‑1 (MQ=255)
tttGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGAt > 2:832686/1‑81 (MQ=255)
tttGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGAt < 2:832645/81‑1 (MQ=255)
gATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGATACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATcgcagcg > 1:832671/1‑81 (MQ=255)
aTAACGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATcgcagcg > 2:832675/1‑80 (MQ=255)
aCGTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATcgcagcgcag > 1:832678/1‑80 (MQ=255)
gTTCAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCAc > 1:832682/1‑81 (MQ=255)
cAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGcc > 1:832699/1‑81 (MQ=255)
cAGGATCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGcc > 1:832674/1‑81 (MQ=255)
aTCGCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTg < 1:832646/81‑1 (MQ=255)
gCATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCgg > 2:832647/1‑81 (MQ=255)
cATCGGTATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCgg > 1:832684/1‑80 (MQ=255)
tATTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACggg < 2:832648/81‑1 (MQ=255)
aTTTTTCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGt > 2:832692/1‑81 (MQ=255)
tttttCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGtt < 1:832649/81‑1 (MQ=255)
tCAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCg > 1:832718/1‑81 (MQ=255)
cAGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGt > 1:832707/1‑81 (MQ=255)
aGCGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTg > 1:832681/1‑81 (MQ=255)
cGGAAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGc < 2:832650/81‑1 (MQ=255)
|
GTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGC > NC_000913/4516788‑4516943
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
