Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,565,149 |
C→T |
Q234Q (CAG→CAA) |
yjiM ← |
putative dehydratase subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,565,149 | 0 | C | T | 100.0%
| 133.8
/ NA
| 38 | Q234Q (CAG→CAA) | yjiM | putative dehydratase subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/18); total (20/18) |
AATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACC > NC_000913/4565071‑4565225
|
aaTCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTg > 2:841881/1‑81 (MQ=255)
gcACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCcttctt < 1:841829/81‑1 (MQ=255)
ccacTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTccc < 2:841830/81‑1 (MQ=255)
ttttCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCActgc > 1:841880/1‑81 (MQ=255)
tttCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCActgct > 1:841871/1‑81 (MQ=255)
ttCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCActgct > 2:841866/1‑80 (MQ=255)
ttCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCActgct > 1:841860/1‑80 (MQ=255)
tgctgcGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGAc > 2:841876/1‑80 (MQ=255)
ctgcGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGaa > 1:841877/1‑81 (MQ=255)
gcgcCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAAcg > 1:841875/1‑81 (MQ=255)
gcgcCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAAcg > 1:841869/1‑81 (MQ=255)
cgccgcCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAAc < 1:841831/79‑1 (MQ=255)
ccgccAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACgcgg < 2:841832/81‑1 (MQ=255)
ccgccAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACgcgg > 2:841867/1‑81 (MQ=255)
ccgccAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACgcgg > 1:841874/1‑81 (MQ=255)
cgccAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACgcggg > 1:841872/1‑81 (MQ=255)
gccAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACgcgggc < 2:841833/81‑1 (MQ=255)
gccAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACgcgggc < 2:841835/81‑1 (MQ=255)
gccAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACgcgggc < 1:841834/81‑1 (MQ=255)
gccAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACgcgggc > 2:841873/1‑81 (MQ=255)
ccAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACgcgggcg > 2:841883/1‑81 (MQ=255)
cAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACgcgggcgg < 1:841836/81‑1 (MQ=255)
gggCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCAt < 2:841837/80‑1 (MQ=255)
gCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGc < 2:841838/81‑1 (MQ=255)
aGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCAt < 1:841839/81‑1 (MQ=255)
ccGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATcc < 1:841840/81‑1 (MQ=255)
tGATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGtt < 1:841841/81‑1 (MQ=255)
gATTAAAATGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGtt > 2:841882/1‑80 (MQ=255)
aaaaTGCGCGGACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCAtt < 1:841842/80‑1 (MQ=255)
ggACGCGGGTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGcc > 2:841906/1‑81 (MQ=255)
gggTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTa < 2:841843/81‑1 (MQ=255)
gggTCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTa > 1:841892/1‑81 (MQ=255)
tCCAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATc > 1:841889/1‑80 (MQ=255)
ccAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCg > 2:841891/1‑80 (MQ=255)
cAGTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGa < 1:841844/80‑1 (MQ=255)
gTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAAcc < 2:841846/81‑1 (MQ=255)
gTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAAcc < 1:841845/81‑1 (MQ=255)
gTCGTTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAAcc > 2:841900/1‑81 (MQ=255)
|
AATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACC > NC_000913/4565071‑4565225
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
