Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,611,176 |
A→G |
intergenic (+173/‑220) |
prfC → / → osmY |
peptide chain release factor RF3/periplasmic chaperone OsmY |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,611,176 | 0 | A | G | 100.0%
| 68.3
/ NA
| 20 | intergenic (+173/‑220) | prfC/osmY | peptide chain release factor RF3/periplasmic chaperone OsmY |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/7); total (13/7) |
TGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAG > NC_000913/4611100‑4611253
|
tGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTGAAt < 2:850404/80‑1 (MQ=255)
gTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACTAAGTGATGACATTTCTGACGGCGTTGAATAc > 2:850420/1‑81 (MQ=255)
cGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTGAATACCGtt < 1:850405/80‑1 (MQ=255)
atatCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATg < 2:850406/80‑1 (MQ=255)
tCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATGCGt < 2:850407/80‑1 (MQ=255)
ccGAGCGGTTTCTAAATTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATGCg > 1:850444/1‑77 (MQ=255)
ggTTTCAAAATTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAg > 2:850436/1‑81 (MQ=255)
cAAAATTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAGTATc > 1:850438/1‑80 (MQ=255)
aaaaTTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAGTATCt > 1:850442/1‑80 (MQ=255)
aaTTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAGTATCTa > 1:850427/1‑79 (MQ=255)
aTTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAGTATCTAAAg > 1:850430/1‑81 (MQ=255)
aTTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAGTATCTAAAg < 1:850408/81‑1 (MQ=255)
tatTTAACAAAGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCg < 2:850409/76‑1 (MQ=255)
tAACAAAGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCAtt > 2:850432/1‑81 (MQ=255)
aCAAAGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCt < 2:850410/81‑1 (MQ=255)
aaGTGATGACATTTCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTAcc > 1:850440/1‑81 (MQ=255)
gaCATTTCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATAtt > 1:850434/1‑80 (MQ=255)
cATTTCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATtaa > 1:850435/1‑80 (MQ=255)
ttCTGACGGCGTTGAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAAc > 2:850418/1‑81 (MQ=255)
gTTGAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCagag > 1:850431/1‑81 (MQ=255)
|
TGTTTTCGCTGATATCCCGAGCGGTTTCAAAATTGTGATCTATATTTAACAAAGTGATGACATTTCTGACGGCGTTAAATACCGTTCAATGCGTAGATATCAGTATCTAAAGCCGTCGATTGTCATTCTACCGATATTAATAACTGATTCAGAG > NC_000913/4611100‑4611253
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
