Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,053,227 |
C→T |
H395Y (CAC→TAC) |
hemN → |
coproporphyrinogen III dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,053,227 | 0 | C | T | 100.0%
| 115.6
/ NA
| 32 | H395Y (CAC→TAC) | hemN | coproporphyrinogen III dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/16); total (16/16) |
ATTCGCCGCGATGTGATTAAGTCGCTCATCTGCAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGCACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCAAAAGATGGGCTGGTGGATGTGGAT > NC_000913/4053149‑4053301
|
aTTCGCCGCGATGTGATTAAGTCGCTCATCTGCAACTTCCGTCTGGATTACGCTCCTATTGAGAAACAGTGGGATTTGTAc > 2:798673/1‑81 (MQ=255)
cgccgcGATGTGATTAAGTCGCTCATCTGCAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACtt < 2:798617/80‑1 (MQ=255)
cgccgcGATGTGATTAAGTCGCTCATCTGCAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACtt < 1:798616/80‑1 (MQ=255)
ccgcGATGTGATTAAGTCGCTCATCTGCAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGc < 2:798618/81‑1 (MQ=255)
tgtgATTAAGTCGCTCATCTGCAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTa < 1:798619/81‑1 (MQ=255)
tgATTAAGTCGCTCATCTGCAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTAc < 2:798620/80‑1 (MQ=255)
tgATTAAGTCGCTCATCTGCAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACt < 2:798621/81‑1 (MQ=255)
ttAAGTCGCTCATCTGCAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTg < 1:798622/81‑1 (MQ=255)
cTCATCTGCAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCgg > 2:798664/1‑76 (MQ=255)
cATCTGCAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGAtct > 1:798680/1‑81 (MQ=255)
tGCAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAg > 1:798676/1‑81 (MQ=255)
cAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAgc < 1:798623/80‑1 (MQ=255)
aaCTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAgctg < 1:798624/81‑1 (MQ=255)
ttCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTc > 2:798667/1‑81 (MQ=255)
ccGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCg < 1:798625/80‑1 (MQ=255)
cGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGcc > 2:798668/1‑81 (MQ=255)
tCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGcccc < 2:798626/81‑1 (MQ=255)
gATTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTa < 2:798627/81‑1 (MQ=255)
aTTACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAg > 1:798696/1‑81 (MQ=255)
ttACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAg > 1:798670/1‑80 (MQ=255)
ttACGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGc < 2:798628/81‑1 (MQ=255)
aCGCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCa > 1:798684/1‑80 (MQ=255)
gCCCCTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCaa > 1:798669/1‑79 (MQ=255)
cccTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCAAAAGa < 1:798629/81‑1 (MQ=255)
ccTATTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCAAAAGAt < 2:798630/81‑1 (MQ=255)
aTTGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCAAAAGATggg > 1:798675/1‑81 (MQ=255)
ttGAGAAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCAAAAGATGGGc > 1:798674/1‑81 (MQ=255)
gagaAACAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCAAAAGATGGGCtg > 2:798634/1‑81 (MQ=255)
aaaCAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCAAAAGATGGGCtggtg > 1:798671/1‑81 (MQ=255)
aaCAGTGGGATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCAAAAGATGGGCtggtg > 2:798685/1‑80 (MQ=255)
gATTTGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCAAAAGATGGGCTGgtggatgtggat < 1:798631/81‑1 (MQ=255)
ttGTACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCAAAAGATGGGCTGgtggatgtggat > 2:798672/1‑78 (MQ=255)
|
ATTCGCCGCGATGTGATTAAGTCGCTCATCTGCAACTTCCGTCTGGATTACGCCCCTATTGAGAAACAGTGGGATTTGCACTTCGCTGATTACTTTGCGGAAGATCTCAAGCTGCTCGCCCCGTTAGCAAAAGATGGGCTGGTGGATGTGGAT > NC_000913/4053149‑4053301
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
