Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,139,431 |
A→G |
L93P (CTG→CCG) |
fsaB ← |
fructose‑6‑phosphate aldolase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,139,431 | 0 | A | G | 100.0%
| 125.2
/ NA
| 34 | L93P (CTG→CCG) | fsaB | fructose‑6‑phosphate aldolase 2 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/8); total (26/8) |
TAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCAT > NC_000913/4139355‑4139507
|
taaCCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGAcc > 2:819295/1‑81 (MQ=255)
cTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCgg < 1:819264/81‑1 (MQ=255)
gtgCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGaa < 2:819265/80‑1 (MQ=255)
tgCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGt > 1:819318/1‑81 (MQ=255)
gcggcgCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGt > 2:819302/1‑80 (MQ=255)
cggcgCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCa > 2:819293/1‑81 (MQ=255)
gcgcTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCAcc < 1:819266/81‑1 (MQ=255)
gcgcTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCAcc < 2:819267/81‑1 (MQ=255)
tatataCAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGa > 1:819317/1‑81 (MQ=255)
ataCAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑ttt > 2:819285/1‑81 (MQ=255)
aCAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑tttt > 1:819301/1‑80 (MQ=255)
aCAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑tttt > 1:819314/1‑80 (MQ=255)
gcagTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTcac > 2:819290/1‑80 (MQ=255)
tGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACaa > 2:819326/1‑81 (MQ=255)
cAAGTGTAGTAATACCCTCATTATTCAGTATTTTAATTGCTGCCGAACCTTCGAAAGTCACCGGGA‑TTTTCACCACAATAc < 1:819268/81‑1 (MQ=255)
aaGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATAcc > 2:819306/1‑81 (MQ=255)
agtaATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGaa > 1:819320/1‑80 (MQ=255)
gtaATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAAt < 2:819269/80‑1 (MQ=255)
taATACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAg > 2:819307/1‑81 (MQ=255)
aaTACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGc > 1:819305/1‑81 (MQ=255)
aTACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGc < 1:819271/80‑1 (MQ=255)
aTACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCg < 2:819270/81‑1 (MQ=255)
tACCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGt > 2:819297/1‑81 (MQ=255)
aCCCTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGTc > 1:819304/1‑81 (MQ=255)
ccTCTTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGTcgc > 1:819321/1‑81 (MQ=255)
ctctTTTTTCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGTcgcg > 2:819315/1‑81 (MQ=255)
tCAGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGTCGCGCAGGcgct > 1:819319/1‑81 (MQ=255)
aGTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGTCGCGCAGGcgcttc > 1:819353/1‑81 (MQ=255)
gTATTTTAATTGCTGCCGGACCTTCGGAAGTCACCGGGATTTTTCACCACAATACCCGGAATAGCGTCGCGCAGGcgcttc > 1:819308/1‑81 (MQ=255)
ttAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGcttct > 2:819312/1‑81 (MQ=255)
tAATTGCTGCCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGcttcta > 1:819329/1‑80 (MQ=255)
gctgcCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTccac > 1:819303/1‑80 (MQ=255)
gctgcCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTccac > 1:819346/1‑80 (MQ=255)
tgcCGGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCAt > 2:819310/1‑81 (MQ=255)
|
TAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGA‑TTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCAT > NC_000913/4139355‑4139507
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
