Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,144,522 |
G→A |
M176I (ATG→ATA) |
pflD → |
putative formate acetyltransferase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,144,522 | 0 | G | A | 100.0%
| 134.4
/ NA
| 37 | M176I (ATG→ATA) | pflD | putative formate acetyltransferase 2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/19); total (18/19) |
ATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTACTGCTGGAAGCCTCGCAGAAAC > NC_000913/4144446‑4144598
|
aTAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATacagc < 1:820583/81‑1 (MQ=255)
aaGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCaa < 2:820584/80‑1 (MQ=255)
aaGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCaa > 2:820585/1‑80 (MQ=255)
ggCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACAt > 2:820623/1‑81 (MQ=255)
cAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGt < 1:820586/81‑1 (MQ=255)
ggggCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGt > 2:820614/1‑79 (MQ=255)
acaTTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCaa > 2:820617/1‑80 (MQ=255)
ttattattGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGc > 1:820627/1‑81 (MQ=255)
aTTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAAt < 2:820587/80‑1 (MQ=255)
cccACGCCTGTTGAATCGCGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACt < 1:820588/80‑1 (MQ=255)
cccACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACtt > 2:820631/1‑81 (MQ=255)
aCGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTa < 2:820589/81‑1 (MQ=255)
cGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTAt < 2:820590/81‑1 (MQ=255)
cTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAg > 1:820628/1‑81 (MQ=255)
gTTGAATCACGGGCTGGGTGAGCTGGCGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGc > 2:820621/1‑81 (MQ=255)
tGAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAACCACTTTTATCAGGCcg > 2:820645/1‑81 (MQ=255)
gAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCcgc > 1:820638/1‑81 (MQ=255)
gAATCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCcg < 1:820591/80‑1 (MQ=255)
aaTCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCcgcg > 2:820641/1‑81 (MQ=255)
aaTCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCcgcg < 1:820592/81‑1 (MQ=255)
aTCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCATCAGCCGGAGAATCACTTTTATCAGGCCGCgt > 2:820644/1‑81 (MQ=255)
aTCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCgt < 2:820594/81‑1 (MQ=255)
aTCACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCgt < 1:820593/81‑1 (MQ=255)
cACGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCgttg > 2:820624/1‑81 (MQ=255)
aCGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCgttgt < 2:820595/81‑1 (MQ=255)
aCGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCgttgt > 1:820634/1‑81 (MQ=255)
cGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCgttgtt < 2:820596/81‑1 (MQ=255)
cGGGCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCgttgtt < 2:820597/81‑1 (MQ=255)
ggCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTAc < 1:820598/81‑1 (MQ=255)
gCTGGGTGAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTAct < 2:820599/81‑1 (MQ=255)
gAGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTACTGCTgg > 2:820630/1‑79 (MQ=255)
aGCTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTACTGCTGGAAg < 1:820600/81‑1 (MQ=255)
cTGGTGGCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTACTGCTGGAAGcc < 2:820601/81‑1 (MQ=255)
ggtggCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTACTGCTGGAAGCCTc > 2:820635/1‑81 (MQ=255)
gCACAGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTACTGCTGGAAGCCTCGCa < 2:820602/80‑1 (MQ=255)
acaGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTACTGCTGGAAGCCTCGCAGa > 1:820629/1‑80 (MQ=255)
acaGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTACTGCTGGAAGCCTCGCAGa > 1:820652/1‑80 (MQ=255)
aGATACAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTACTGCTGGAAGCCTCGCAGAAac < 2:820603/81‑1 (MQ=255)
|
ATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTACTGCTGGAAGCCTCGCAGAAAC > NC_000913/4144446‑4144598
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
