Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,460,132 |
T→C |
H78R (CAT→CGT) |
nrdG ← |
anaerobic ribonucleoside‑triphosphate reductase activating protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,460,132 | 0 | T | C | 100.0%
| 112.2
/ NA
| 31 | H78R (CAT→CGT) | nrdG | anaerobic ribonucleoside‑triphosphate reductase activating protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/15); total (16/15) |
ACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCT > NC_000913/4460057‑4460209
|
acCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGc > 2:895648/1‑81 (MQ=255)
gATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATc < 2:895601/81‑1 (MQ=255)
cTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATcgccgc > 2:895642/1‑81 (MQ=255)
aCCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCgg < 2:895602/80‑1 (MQ=255)
ccGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGga > 1:895660/1‑79 (MQ=255)
cacTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGa > 1:895666/1‑80 (MQ=255)
cTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAAt < 2:895603/81‑1 (MQ=255)
cGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATAc > 2:895652/1‑81 (MQ=255)
cgcgGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCt > 1:895663/1‑81 (MQ=255)
gcgGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTg > 1:895662/1‑81 (MQ=255)
gcgGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTg > 1:895661/1‑81 (MQ=255)
cgGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTgg < 2:895604/81‑1 (MQ=255)
aTGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGt > 2:895649/1‑81 (MQ=255)
gCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGttt > 1:895643/1‑81 (MQ=255)
gCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGttt > 2:895650/1‑81 (MQ=255)
cGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGttt < 2:895605/80‑1 (MQ=255)
cGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGttt < 1:895606/80‑1 (MQ=255)
gTACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATa < 2:895607/80‑1 (MQ=255)
tACCAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATAc < 2:895608/80‑1 (MQ=255)
ccAGTTTCAGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGa < 2:895609/80‑1 (MQ=255)
aGAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCAtt > 2:895655/1‑80 (MQ=255)
gAATATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCAtt > 1:895668/1‑79 (MQ=255)
aaTATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAg < 1:895610/81‑1 (MQ=255)
aaTATCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAg < 2:895611/81‑1 (MQ=255)
tatCCGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGAt < 2:895612/81‑1 (MQ=255)
ccGGCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGt > 2:895667/1‑81 (MQ=255)
gCACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTaa < 2:895613/81‑1 (MQ=255)
cACGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAAt > 1:895658/1‑81 (MQ=255)
cGTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGa < 2:895614/81‑1 (MQ=255)
gTTTTGCGGACGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGAt > 1:895657/1‑81 (MQ=255)
ggACGCAGCGGATCGCCGCCGGAGTGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCt < 2:895615/81‑1 (MQ=255)
|
ACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCT > NC_000913/4460057‑4460209
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
