Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,590,284 |
G→A |
F332F (TTC→TTT) |
btsT ← |
pyruvate:H(+) symporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,590,284 | 0 | G | A | 100.0%
| 161.5
/ NA
| 44 | F332F (TTC→TTT) | btsT | pyruvate:H(+) symporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/27); total (17/27) |
ATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTC > NC_000913/4590206‑4590359
|
aTTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATaaa < 1:923884/81‑1 (MQ=255)
tAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATaaa > 2:923945/1‑79 (MQ=255)
gCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAg > 1:923959/1‑80 (MQ=255)
ccAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGa < 1:923885/81‑1 (MQ=255)
cagcagTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGaa < 2:923886/81‑1 (MQ=255)
agcagTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAAc > 1:923955/1‑81 (MQ=255)
cagTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACgg < 2:923887/81‑1 (MQ=255)
agTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACgg > 1:923949/1‑80 (MQ=255)
gTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGa < 2:923888/81‑1 (MQ=255)
cgtcgtACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAgc < 1:923889/81‑1 (MQ=255)
gtACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGcc < 1:923890/80‑1 (MQ=255)
gtACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCt > 1:923891/1‑81 (MQ=255)
aCCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCttt > 1:923973/1‑81 (MQ=255)
gAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCcac < 1:923892/81‑1 (MQ=255)
aaGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCcaca < 2:923894/81‑1 (MQ=255)
aaGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCcaca < 1:923893/81‑1 (MQ=255)
gagaTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGc < 1:923895/81‑1 (MQ=255)
agaTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCg > 1:923896/1‑81 (MQ=255)
gaTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCgg > 2:923976/1‑81 (MQ=255)
aGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCa < 1:923897/81‑1 (MQ=255)
cgcgTGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGt < 2:923898/81‑1 (MQ=255)
gTGGAAGCCAGATACCGCACCACAGGCGATGGTTATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTAcc > 1:923970/1‑81 (MQ=255)
tGGAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCg > 2:923962/1‑81 (MQ=255)
gAAGCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTc < 1:923899/81‑1 (MQ=255)
gCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAAt > 2:923974/1‑81 (MQ=255)
gCCAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAAt < 1:923900/81‑1 (MQ=255)
cAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATg > 1:923969/1‑80 (MQ=255)
cAGATACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGt < 2:923901/81‑1 (MQ=255)
aTACCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACt < 1:923902/81‑1 (MQ=255)
aCCGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTgg > 2:923977/1‑81 (MQ=255)
ccGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTgg < 1:923903/80‑1 (MQ=255)
cGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGt < 1:923906/81‑1 (MQ=255)
cGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGt < 1:923905/81‑1 (MQ=255)
cGCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGt < 2:923904/81‑1 (MQ=255)
gCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGt > 1:923965/1‑80 (MQ=255)
gCACCACAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTc < 1:923907/81‑1 (MQ=255)
accacAGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCAt > 2:923982/1‑81 (MQ=255)
cacaGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATgg < 1:923908/81‑1 (MQ=255)
acaGGCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATggc < 2:923909/81‑1 (MQ=255)
ggCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTGTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTTATggcagg < 2:923910/81‑1 (MQ=37)
gCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATggcagg > 2:923972/1‑80 (MQ=255)
gCGATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATggcagg > 2:923980/1‑80 (MQ=255)
gATGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCAt > 2:923975/1‑81 (MQ=255)
tGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCAttt < 1:923911/81‑1 (MQ=255)
tGGTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGAGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCAttt < 2:923912/81‑1 (MQ=255)
gTGATAAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTc < 1:923913/81‑1 (MQ=255)
|
ATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTC > NC_000913/4590206‑4590359
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
