Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	169	80.7	2736954	99.0%	2709584	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,933,347	T→C	T420A (ACA→GCA)	edd ←	phosphogluconate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,933,347	0	T	C	100.0%	170.9 / NA	47	T420A (ACA→GCA)	edd	phosphogluconate dehydratase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/17); total (30/17)

ACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACC  >  NC_000913/1933213‑1933476

aCCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACTCTTTTGCCccac                                                                                                                              >  2:1238456/1‑140 (MQ=255)

aCCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCccac                                                                                                                              >  1:1179051/1‑140 (MQ=255)

 cccGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCccacc                                                                                                                             <  1:567669/140‑1 (MQ=255)

  ccGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCccacca                                                                                                                            >  1:829609/1‑140 (MQ=255)

  ccGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCccacca                                                                                                                            >  1:706391/1‑140 (MQ=255)

  ccGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCccacca                                                                                                                            <  2:531256/140‑1 (MQ=255)

    gCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatg                                                                                                                          >  1:853781/1‑140 (MQ=255)

      ttCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgat                                                                                                                        <  2:1039676/140‑1 (MQ=255)

      ttCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgat                                                                                                                        <  1:1171614/140‑1 (MQ=255)

      ttCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgat                                                                                                                        <  1:889552/140‑1 (MQ=255)

      ttCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgat                                                                                                                        <  1:1333724/140‑1 (MQ=255)

      ttCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgat                                                                                                                        >  1:783779/1‑140 (MQ=255)

      ttCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgat                                                                                                                        <  2:1361821/140‑1 (MQ=255)

       tCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCatgatg                                                                                                                       >  1:1135213/1‑140 (MQ=255)

           aGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATgagag                                                                                                                   <  1:411807/140‑1 (MQ=255)

           aGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATgagag                                                                                                                   <  1:674538/140‑1 (MQ=255)

                ggCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAgg                                                                                                              >  1:68720/1‑140 (MQ=255)

                        gTCATGCTGCTTTTCAAAATCAACCGCTGGCGCTTCAATCTCCTGGGTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACGCTTTTGCCCCTCCATGATGAGAGAAAGGTTGTTCga                                                                                                      <  2:829609/140‑1 (MQ=255)

                           aTGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCgaagg                                                                                                   >  2:1316293/1‑140 (MQ=255)

                            tGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCgaagga                                                                                                  <  2:853781/140‑1 (MQ=255)

                               tGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACTCTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGc                                                                                               <  1:1238456/140‑1 (MQ=255)

                               tGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGc                                                                                               <  1:1302626/140‑1 (MQ=255)

                                 gCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGa                                                                                             >  1:257921/1‑140 (MQ=255)

                                  cTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGAt                                                                                            <  2:68720/140‑1 (MQ=255)

                                  cTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGAt                                                                                            >  1:808317/1‑140 (MQ=255)

                                    ttCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATca                                                                                          <  2:808317/140‑1 (MQ=255)

                                             aacCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGt                                                                                 >  1:1148730/1‑140 (MQ=255)

                                               ccGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCg                                                                               >  2:1288953/1‑140 (MQ=255)

                                                                 ccTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGc                                                                 >  1:1046031/1‑136 (MQ=255)

                                                                 ccTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGc                                                                 <  2:68030/136‑1 (MQ=255)

                                                                 ccTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGc                                                                 <  2:1046031/136‑1 (MQ=255)

                                                                 ccTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGc                                                                 >  1:68030/1‑136 (MQ=255)

                                                                  cTGTTTCTCAACCGGCACGGCAGAGTTTTTCATACCCGCCCGGCCCAGGTTACCGCTTAACCCTTTTCCCCCACCATGATGAGAAAAGGGTTGTTCGAAGGAAGCGATCACATTGCTGTGGAGTGTTTTTCCCGCCCCtt                                                            <  2:257921/140‑1 (MQ=255)

                                                                   tGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTc                                                           >  1:232737/1‑140 (MQ=255)

                                                                       tctcACCCGGCACGGCAGAGGTTTTCATACCCGCACGGCCAAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAAAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGTTTTTTCCGCCCCTTCCCGc                                                       <  2:485601/140‑1 (MQ=255)

                                                                        ctcAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGcc                                                      >  2:2762/1‑140 (MQ=255)

                                                                            aCCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtc                                                  >  2:988657/1‑140 (MQ=255)

                                                                                gcacggcaGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtccagt                                              >  1:602979/1‑140 (MQ=255)

                                                                                gcacggcaGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtccagt                                              >  2:522494/1‑140 (MQ=255)

                                                                                 cacggcaGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAAAAAGTTTGTCCGAAGGAAGCGATCACATTGCTGTGGAGTGTTTTTTCCGCCCCTTCCCGCCAGTCCAGtt                                             <  2:706391/140‑1 (MQ=255)

                                                                                           tttttCATACCCGCACGGCCCAGGTTACCGCTTAACCTTTTTCCCCCACCATGATGAGAGAAGGGTTGTTCGAGGGAAGCGATCACTTTGCTGTCGAGTGTTTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCattatt                                   <  2:1135213/139‑1 (MQ=255)

                                                                                                cATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGTTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGTTTTTTCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGcc                              <  2:1179051/140‑1 (MQ=255)

                                                                                                     ccGCACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGt                         >  1:1006215/1‑140 (MQ=255)

                                                                                                        cACGGCCCGGGTTACCGCTTAACATTTTTCCCCCCCCATGATGAGAGAAAGTTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTTTTTTTTCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCa                      <  2:602979/140‑1 (MQ=255)

                                                                                                        cACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCa                      >  2:449900/1‑140 (MQ=255)

                                                                                                        cACGGCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCa                      >  1:1160752/1‑140 (MQ=255)

                                                                                                            gCCCAGGTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGTTTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAggg                  <  2:1148730/140‑1 (MQ=255)

                                                                                                                 ggTTACCGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATaa             >  1:1281134/1‑140 (MQ=255)

                                                                                                                       cGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCTAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac       >  2:634640/1‑140 (MQ=255)

                                                                                                                       cGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac       >  1:582102/1‑140 (MQ=255)

                                                                                                                       cGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac       >  2:24459/1‑140 (MQ=255)

                                                                                                                       cGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac       >  2:728989/1‑140 (MQ=255)

                                                                                                                       cGCTTAACACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac       >  2:759353/1‑140 (MQ=255)

                                                                                                                            aaCACTTTTGCCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGAcc  >  1:1354269/1‑140 (MQ=255)

ACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAATCACCTGGTTCTCAACCGGCACGGCAGAGGTTTTCATAACCGCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACC  >  NC_000913/1933213‑1933476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A